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Binding of galectin-1 (gal-1) to the Thomsen–Friedenreich (TF) antigen on trophoblast cells and inhibition of proliferation of trophoblast tumor cells in vitro by gal-1 or an anti-TF antibody

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Abstract

Galectin-1 (gal-1), a member of the mammalian β-galactoside-binding proteins, recognizes preferentially Galβ1-4GlcNAc sequences of several cell surface oligosaccharides. We demonstrate histochemically that the lectin recognizes appropriate glycotopes on the syncytiotrophoblast and extravillous trophoblast layer from second trimester human placenta and on BeWo chorion carcinoma cells. Gal-1 binding to BeWo cells was diminished by the Thomsen–Friedreich (TF)-disaccharide (Galβ1-3GalNAc-) conjugated to polyacrylamide (TF–PAA). Gal-1 also inhibited BeWo cell proliferation in a concentration-dependent manner. Similar antiproliferative effects were also observed with an anti-TF monoclonal antibody (mAb, A78-G/A7). Therefore, we conclude that ligation of Galβ1-4GlcNAc and Galβ1-3GalNAc epitopes on BeWo cells may have regulatory effects on cell proliferation.

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Acknowledgments

We thank D.U. Richter and G. Gaede for excellent technical assistance. The study was supported by the “Deutsche Forschungsgemeinschaft” (DFG) for U. Jeschke and H. Walzel.

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Authors and Affiliations

  1. First Department of Obstetrics and Gynaecology, Ludwig Maximilians University of Munich, Maistrasse 11, 80337, Munich, Germany

    Udo Jeschke, Irmi Wiest, Sandra Schulze, Christina Kuhn & Klaus Friese

  2. Max Delbrück Centre for Molecular Medicine, R.-Rössle-Str. 10, 13125, Berlin, Germany

    Uwe Karsten

  3. Institute of Medical Biochemistry and Molecular Biology, University of Rostock, Schillingallee 70, 18057, Rostock, Germany

    Hermann Walzel

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  1. Udo Jeschke
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  2. Uwe Karsten
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Correspondence to Udo Jeschke.

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Jeschke, U., Karsten, U., Wiest, I. et al. Binding of galectin-1 (gal-1) to the Thomsen–Friedenreich (TF) antigen on trophoblast cells and inhibition of proliferation of trophoblast tumor cells in vitro by gal-1 or an anti-TF antibody. Histochem Cell Biol 126, 437–444 (2006). https://doi.org/10.1007/s00418-006-0178-1

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