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The evaluation of diagnostic efficiency for stromal herpes simplex keratitis by the combination of tear HSV-sIgA and HSV-DNA

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Abstract

Purpose

To assess the differential diagnostic values for stromal herpes simplex keratitis (HSK) by using tear HSV-sIgA, tear HSV-DNA, and the combination.

Methods

Tear samples for both eyes and the paired serum were collected from 187 stromal HSK and 56 controls. Enzyme-linked immune sorbent assay (ELISA) was used to analyze the tear HSV-sIgA and serum IgG/IgM/IgA. The levels of tear HSV-DNA were measured by polymerase chain reaction (PCR).

Results

The positive rates for tear HSV-sIgA and HSV-DNA were 36.90% and 10.96% respectively in stromal HSK patients. Twelve showed positivity for both sIgA and DNA, while 46 cases were positive for sIgA or DNA. The sensitivity, specificity, PPV, and NPV for simultaneous measurement were 39.73%, 98.21%, 98.31%, and 38.46%. The total negative conversion rate of sIgA was 95.71%.

Conclusions

The diagnostic efficiency of HSV-sIgA only is nearly equal to the combination of HSV-sIgA and HSV-DNA, and the positive result is optimum to achieve a reliable diagnosis of stromal HSK even in atypical or unsuspected cases.

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Acknowledgements

This work was financially supported by Shanghai Committee of Science and Technology Foundation (134119a1900).

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Authors and Affiliations

  1. Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China

    Jini Qiu, Feifei Huang, Zhujian Wang, Jianjiang Xu & Chaoran Zhang

  2. The Key Lab of Myopia, Ministry of Health, Shanghai, People’s Republic of China

    Jini Qiu, Feifei Huang, Jianjiang Xu & Chaoran Zhang

Authors
  1. Jini Qiu
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  2. Feifei Huang
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  3. Zhujian Wang
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  4. Jianjiang Xu
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  5. Chaoran Zhang
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Corresponding author

Correspondence to Chaoran Zhang.

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Funding

Shanghai Committee of Science and Technology Foundation provided financial support in the form of biomedical funding (134119a1900). The sponsor had no role in the design or conduct of this research.

Conflict of interest

All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge, or beliefs) in the subject matter or materials discussed in this manuscript.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Jini Qiu and Feifei Huang contributed equally to the work presented here, and should therefore be regarded as equivalent authors.

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Qiu, J., Huang, F., Wang, Z. et al. The evaluation of diagnostic efficiency for stromal herpes simplex keratitis by the combination of tear HSV-sIgA and HSV-DNA. Graefes Arch Clin Exp Ophthalmol 255, 1409–1415 (2017). https://doi.org/10.1007/s00417-017-3653-6

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  • DOI: https://doi.org/10.1007/s00417-017-3653-6

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