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ANGPTL-4 correlates with vascular endothelial growth factor in patients with proliferative diabetic retinopathy

  • Retinal Disorders
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

Purpose

To investigate the relationship between angiopoietin-like protein 4 (ANGPTL-4) and vascular endothelial growth factor (VEGF) in the serum and vitreous of eyes in patients with proliferative diabetic retinopathy (PDR).

Methods

Thirty-five eyes of 35 patients with PDR, 20 eyes of 20 patients with non-proliferative diabetic retinopathy, 20 eyes of 20 patients with diabetes but no diabetic retinopathy, and 14 eyes of 14 nondiabetic patients with an idiopathic macular hole (IMH) were recruited from Shanghai First People’s Hospital. The ANGPTL-4 and VEGF concentrations were determined using enzyme-linked immunosorbent assays. Group means were compared using one-way analysis of variance with GraphPad Prism 4.0 and SPSS ver. 17.0. The research followed the tenets of the Declaration of Helsinki.

Results

The ANGPTL-4 and VEGF levels were significantly higher in the vitreous and serum of patients with PDR compared with patients with IMH. There were significant correlations between the ANGPTL-4 and VEGF levels in the vitreous and serum of patients with PDR. The vitreous and serum ANGPTL-4 levels were also significantly correlated in patients with PDR. The ANGPTL-4 in both the vitreous and serum correlated with the serum triglyceride and high-density lipoprotein cholesterol levels.

Conclusions

The ANGPTL-4 levels were markedly elevated and the ANGPTL-4 expression was directly correlated with the VEGF expression in the vitreous and serum of patients with PDR. The vitreous and serum ANGPTL-4 levels were also significantly correlated with serum lipids in patients with PDR. Our results suggest that the ANGPTL-4 may be used as a new therapeutic target for the treatment of PDR.

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Acknowledgments

This work was supported by grants from the National Nature Science Foundation of China (81271032). The sponsor had no role in the design or conduct of this research.

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Author notes

    Authors and Affiliations

    1. Department of Ophthalmology, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong University, No. 100 Haining Road, Shanghai, 200080, China

      Qianyi Lu, Chen Zou & Zhi Zheng

    2. Department of Ophthalmology, Nanjing Medical University Affiliated Wuxi Second Hospital, No. 68 Zhongshan Road, Wuxi, 214002, China

      Wenjun Zou

    3. Department of Ophthalmology, Changshu No. 1 People’s Hospital, Changshu, 215500, China

      Bin Chen

    4. Department of Ophthalmology, Nanjing Medical University Affiliated Shanghai General Hospital, Shanghai, 200080, China

      Minjie Zhao

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    1. Qianyi Lu
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    Correspondence to Zhi Zheng.

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    Conflict of interest

    All authors certify that we have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

    Ethical approval

    All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. For this type of study formal consent is not required. Informed consent was obtained from all individual participants included in the study.

    Additional information

    Qianyi Lu and Wenjun Zou contributed equally to this work.

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    Supplementary Table 1

    One-sample Kolmogorov–Smirnov test. (DOC 36 kb)

    Supplementary Table 2

    Pearson’s correlation tests with r and p values between ANGPTL-4 and other factors in PDR patients. (DOC 31 kb)

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    Cite this article

    Lu, Q., Zou, W., Chen, B. et al. ANGPTL-4 correlates with vascular endothelial growth factor in patients with proliferative diabetic retinopathy. Graefes Arch Clin Exp Ophthalmol 254, 1281–1288 (2016). https://doi.org/10.1007/s00417-015-3187-8

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    • DOI: https://doi.org/10.1007/s00417-015-3187-8

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