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Segmental reproducibility of retinal blood flow velocity measurements using retinal function imager

  • Retinal Disorders
  • Published:
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Abstract

Background

To evaluate the reproducibility of blood flow velocity measurements of individual retinal blood vessel segments using retinal function imager (RFI).

Methods

Eighteen eyes of 15 healthy subjects were enrolled prospectively at three centers. All subjects underwent RFI imaging in two separate sessions 15 min apart by a single experienced photographer at each center. An average of five to seven serial RFI images were obtained. All images were transferred electronically to one center, and were analyzed by a single observer. Multiple blood vessel segments (each shorter than 100 μm) were co-localized on first and second session images taken at different times of the same fundus using built-in software. Velocities of corresponding segments were determined, and then the inter-session reproducibility of flow velocity was assessed by the concordance correlation co-efficient (CCC), coefficient of reproducibility (CR), and coefficient of variance (CV).

Results

Inter-session CCC for flow velocity was 0.97 (95% confidence interval (CI), 0.966 to 0.9797). The CR was 1.49 mm/sec (95% CI, 1.39 to 1.59 mm/sec), and CV was 10.9%. The average arterial blood flow velocity was 3.16 mm/sec, and average venous blood flow velocity was 3.15 mm/sec. The CR for arterial and venous blood flow velocity was 1.61 mm/sec and 1.27 mm/sec respectively.

Conclusion

RFI provides reproducible measurements for retinal blood flow velocity for individual blood vessel segments, with 10.9% variability.

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Acknowledgments

This study was supported by an Unrestricted Research Fund to Jacobs Retina Center at Shiley Eye Center, University of California, San Diego (LC), NIH-EY 007366 (WRF), NIH EY 020617 (LC), NIH- EY 018589 (WRF), RPB Inc. New York (WRF), NIH-EY 016323 (DUB) and Retina Research Foundation of Wills Eye Institute (SJG).

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Author notes

  1. Jay Chhablani

    Present address: L V Prasad Eye Institute, Hyderabad, India

Authors and Affiliations

  1. Jacobs Retina Center at Shiley Eye Center, University of California, San Diego, 9415 Campus Point Drive, MC 0946, La Jolla, CA, 92093-0946, USA

    Jay Chhablani, Dirk-Uwe Bartsch, Lingyun Cheng, Laura Gomez & William R. Freeman

  2. The Retina Service, Wills Eye Institute and Thomas Jefferson University, Philadelphia, PA, USA

    Rayan A. Alshareef, Sami S. Rezeq & Sunir J. Garg

  3. Department of Ophthalmology, The Edith Wolfson Medical Center, Holon, Israel, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel

    Zvia Burgansky-Eliash

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  1. Jay Chhablani
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Correspondence to William R. Freeman.

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The authors have full control of all primary data, and we agree to allow Graefe’s Archive for Clinical and Experimental Ophthalmology to review their data upon request.

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Chhablani, J., Bartsch, DU., Cheng, L. et al. Segmental reproducibility of retinal blood flow velocity measurements using retinal function imager. Graefes Arch Clin Exp Ophthalmol 251, 2665–2670 (2013). https://doi.org/10.1007/s00417-013-2360-1

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  • DOI: https://doi.org/10.1007/s00417-013-2360-1

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