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Involvement of advanced glycation end products, oxidative stress and nuclear factor-kappaB in the development of diabetic keratopathy

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Abstract

Background

The purpose of the experiment reported here was to assess the involvement of advanced glycation end products (AGEs), oxidative stress, and nuclear factor kappa-B (NF-κB) activation in the development of diabetic keratopathy.

Methods

Diabetes was induced by intraperitoneal streptozotocin injection in male Sprague–Dawley rats. The thickness of the cornea was measured. Apoptosis was detected by TUNEL assay and western blot for caspase-3. The expression of AGEs and 8-hydroxydeoxyguanosine (8-OHdG) were studied by immunohistochemistry in corneal tissues of normoglycaemic and diabetic rats. NF-κB activation was evaluated by electrophoretic mobility shift assay and southwestern histochemistry.

Results

Corneal edema was observed in diabetic rats. The thickness of cornea was higher in diabetic than in control rats. AGEs were accumulated in corneal tissues. 8-OHdG and NF-κB were identified in corneal epithelium, stroma and endothelium, and its expressions were greater in diabetic than in those of control rats. Diabetes induces significant alterations in rat corneal tissue structure.

Conclusions

The higher expression of AGE, 8-OHdG and NF-κB in corneal tissues of diabetic rats suggests that these factors are involved in apoptosis and in subsequent corneal alterations related to diabetic keratopathy.

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Acknowledgments

This research was supported by a grant [L08010, K09030] from the Korea Institute of Oriental Medicine (KIOM).

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Authors and Affiliations

  1. Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM), 483 Exporo, Yuseong-gu, Daejeon, 305-811, South Korea

    Junghyun Kim, Chan-Sik Kim, Eunjin Sohn, Il-Ha Jeong, Hyojun Kim & Jin Sook Kim

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  1. Junghyun Kim
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Correspondence to Jin Sook Kim.

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Kim, J., Kim, CS., Sohn, E. et al. Involvement of advanced glycation end products, oxidative stress and nuclear factor-kappaB in the development of diabetic keratopathy. Graefes Arch Clin Exp Ophthalmol 249, 529–536 (2011). https://doi.org/10.1007/s00417-010-1573-9

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  • DOI: https://doi.org/10.1007/s00417-010-1573-9

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