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Evaluation of the genetic parameters and mutation analysis of 22 STR loci in the central Chinese Han population

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Abstract

At present, the Han nationality is China’s main ethnic group and also the most populous nation in the world. This is a great resource to study microsatellite mutations and for the study of ethnogeny. The aim of this study is to investigate the genetic polymorphisms and mutations of 22 autosomal STR loci in 2475 individuals from Henan province, China. DNA is amplified and genotyped using PowerPlex™24 system. The gene frequencies, forensic parameters, and the mutation rate of the 22 STR loci are analyzed. A total of 295 alleles are observed in this Henan Han population, and the allelic frequencies ranged from 0.0003 to 0.5036. In order to investigate the genetic relationships between the Henan Han and the other 14 different populations, our present data were compared with previously published data for the same 15 STR loci. The results indicated that the Henan Han had closer genetic relationships the groups including Minnan Han, Maonan, Yi and Guangdong Han groups while the South morocco population, the Moroccan population, the Malay group, and the Uigur stand away from Henan Han. Except of D2S441, D13S317, PentaE, D2S1338, D5S818, TPOX and D19S433, the mutation events are found in the other 15 STR loci. A total of 40 mutation events are observed in the 15 STR loci. The mutation rates are ranged from 0 to 4.85 × 10−3. In this study, 39 mutations are single-step mutations, and only one at FGA comprised two steps. STR mutation is commonly existed in paternity testing, while there are no STR mutation studies of the 22 STR loci in the Henan Han population. It is of great importance in forensic individual discrimination and paternal testing.

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Acknowledgments

This project was supported by the National Natural Science Foundation of China (NSFC, no. 81501336, 81525015) and by the Scientific and technological projects of Henan Province (no. 162102310294). The authors are grateful to Prof. Feng Chen and Dr. Cai-Yong Yin for providing the help for data analysis.

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Authors and Affiliations

  1. Medical Genetic Institute of Henan Province, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, Henan, 450003, People’s Republic of China

    Wang Hongdan, Kang Bing, Su Ning, He Miao, Zhang Bo & Liao Shixiu

  2. Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an, Shaanxi, 710004, People’s Republic of China

    Wang Hongdan, Guo Yuxin & Zhu Bofeng

  3. Clinical Reaserch Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi’an Jiaotong University, Xi’an, Shaanxi, 710004, People’s Republic of China

    Guo Yuxin & Zhu Bofeng

  4. Research Center of Stomatology, Stomatological Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi, 710004, People’s Republic of China

    Guo Yuxin & Zhu Bofeng

  5. School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450000, People’s Republic of China

    Zeng Zhaoshu

Authors
  1. Wang Hongdan
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  2. Kang Bing
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  3. Su Ning
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  4. He Miao
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  5. Zhang Bo
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  6. Guo Yuxin
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  7. Zhu Bofeng
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  8. Liao Shixiu
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  9. Zeng Zhaoshu
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Corresponding authors

Correspondence to Wang Hongdan, Liao Shixiu or Zeng Zhaoshu.

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Hongdan, W., Bing, K., Ning, S. et al. Evaluation of the genetic parameters and mutation analysis of 22 STR loci in the central Chinese Han population. Int J Legal Med 131, 103–105 (2017). https://doi.org/10.1007/s00414-016-1389-1

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  • DOI: https://doi.org/10.1007/s00414-016-1389-1

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