Skip to main content
SpringerLink
Log in

Genetic polymorphisms of 26 Y-STR loci in the Mongolian minority from Horqin district, China

  • Short Communication
  • Published:
International Journal of Legal Medicine Aims and scope Submit manuscript

Abstract

To study the population data of Y chromosome STR (Y-STRs) of the Mongolian minority population residing in the Horqin district, we analyzed haplotypes of 26 Y-STRs (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, DYS388, DYS449, DYS460, and YGATAH4) in 298 unrelated Chinese Mongolian individuals using the commercially available Goldeneye® DNA ID 26Y system. We also investigated blood stains, saliva spots, semen spots, hair follicles, fingernails, and sweat latent fingerprints from ten healthy males for testing the efficiency of direct amplification of this new Y-STRs system. The calculated average gene diversity values of the Mongolian population ranged from 0.3024 to 0.9510 for the DYS389I and DYS385a/b loci, respectively. The discriminatory capacity was 92.95 % with 277 observed haplotypes using 23 Y-STR loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, and YGATAH4). By adding three more Y-STRs (DYS388, DYS449, and DYS460) to the 26Y system, the discriminatory capacity was increased to 94.63 % with a total of 282 observed haplotypes. Population relationships were calculated and compared with seven populations available from the Y chromosome haplotype reference database and data from ten Asian populations published previously. The Mongolian minority population residing in Horqin district is significantly different from other populations. Our results indicated that these 26 Y-STRs were highly genetically polymorphic in the Mongolian group and this contributes greatly to existing Chinese ethnic genetic information. As a result of direct amplification, we have obtained full profile from all blood stains, saliva spots, hair follicles, and fingernails; six semen spots; and one sweat latent fingerprint. It revealed that the 26 Y-STR system was a valuable tool for male sex analysis in forensic field and the kit was highly adaptive to direct amplification of various samples including blood stain, saliva spot, hair follicle, and fingernail.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Population Census Office under the State Council Department of Population and Employment Statistics National Bureau of Statistics (2012) Tabulation of the 2010 Population Census of the People’s Republic of China. China Statistics Press, Beijing

    Google Scholar 

  2. Calvin LK (2010) The Inner Mongolia Autonomous Region: a major role in China’s renewable energy future. Util Policy 18:46–52

    Article  Google Scholar 

  3. Janhunen J (2011) The Mongolic languages. Routledge, London

    Google Scholar 

  4. Jobling MA, Pandya A, Tyler-Smith C (1997) The Y chromosome in forensic analysis and paternity testing. Int J Legal Med 110:118–124

    Article  CAS  Google Scholar 

  5. Zhang S, Tian H, Wang Z, Zhao S, Hu Z, Li C, Ji C (2014) Development of a new 26plex Y-STRs typing system for forensic application. Forensic Sci Int Genet 13:112–120

    Article  CAS  Google Scholar 

  6. Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16:1215

    Article  CAS  Google Scholar 

  7. Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York

    Book  Google Scholar 

  8. Felsenstein J. PHYLIP (phylogeny inference package) version 3.695, Department of Genome Sciences, University of Washington, Seattle, 2009

  9. Page RDM (1996) TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    CAS  PubMed  Google Scholar 

  10. Gusmão L, Butler JM, Carracedo A, Gill P, Kayser M, Mayr WR, Morling N, Prinz M, Roewer L, Tyler-Smith C, Schneider PM (2006) DNA Commission of the International Society of Forensic Genetics (ISFG): an update of the recommendations on the use of Y-STRs in forensic analysis. Int J Legal Med 120:191–200

    Article  Google Scholar 

  11. Parson W, Roewer L (2010) Publication of population data of linearly inherited DNA markers in the International Journal of Legal Medicine. Int J Legal Med 124:505–509

    Article  Google Scholar 

  12. Guo F (2015) Population genetics for 17 Y-STR loci in Mongolian ethnic minority from Liaoning Province, Northeast China. Forensic Sci Int Genet 17:153–154

    Article  CAS  Google Scholar 

  13. Guo F, Zhang L, Jiang X (2015) Population genetics of 17 Y-STR loci in Xibe ethnic minority from Liaoning Province, Northeast China. Forensic Sci Int Genet 16:86–87

    Article  CAS  Google Scholar 

  14. Guo H, Yan J, Jiao Z, Tang H, Zhang Q, Zhao L, Hu N, Li H, Liu Y (2008) Genetic polymorphisms for 17 Y-chromosomal STRs haplotypes in Chinese Hui population. Leg Med 10:163–169

    Article  CAS  Google Scholar 

  15. Yan J, Tang H, Liu Y, Jing Y, Jiao Z, Zhang Q, Gao J, Shang L, Guo H, Yu J (2007) Genetic polymorphisms of 17 Y-STRs haplotypes in Chinese Han population residing in Shandong Province of China. Leg Med 9:196–202

    Article  CAS  Google Scholar 

  16. Vongpaisarnsin K, Saengkaeotrakul P, Boonlert A, Tansrisawad N (2015) Gene and haplotype diversity of 23 Y-chromosomal short tandem repeat loci in the central Thai population. Forensic Sci Int Genet 14:191–193

    Article  CAS  Google Scholar 

  17. Bai R, Zhang Z, Liang Q, Lu D, Yuan L, Yang X, Shi M (2013) Haplotype diversity of 17 Y-STR loci in a Chinese Han population sample from Shanxi Province, Northern China. Forensic Sci Int Genet 7:214–216

    Article  CAS  Google Scholar 

  18. Luo H, Song F, Zhang L, Hou Y (2015) Genetic polymorphism of 23 Y-STR loci in the Zhuang minority population in Guangxi of China. Int J Legal Med 129:737–738

    Article  Google Scholar 

  19. Shan W, Ablimit A, Zhou W, Zhang F, Ma Z, Zheng X (2014) Genetic polymorphism of 17 Y chromosomal STRs in Kazakh and Uighur populations from Xinjiang, China. Int J Legal Med 128:743–744

    Article  Google Scholar 

  20. Huang TY, Hsu YT, Li JM, Chung JH, Shun CT (2008) Polymorphism of 17 Y-STR loci in Taiwan population. Forensic Sci Int 74:249–254

    Article  Google Scholar 

  21. Ye Y, Gao J, Fan G, Liao L, Hou Y (2015) Population genetics for 23 Y-STR loci in Tibetan in China and confirmation of DYS448 null allele. Forensic Sci Int Genet 16:e7–e10

    Article  CAS  Google Scholar 

  22. Bauer M, Patzelt D (2003) A method for simultaneous RNA and DNA isolation from dried blood and semen stains. Forensic Sci Int 136:76–78

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the Science Foundation for the Youth Scholars of Central South University (no. 120925), the Natural Science Foundation for Youth (no. 81302621), and the National Natural Science Foundation of China (81373249 and 81571855).

Author information

Authors and Affiliations

  1. Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, 410013, Hunan, People’s Republic of China

    Xiaoliang Fu, Juanjuan Guo, Yanfang Liu, Yadong Guo, Jie Yan, Jifeng Cai & Lagabaiyila Zha

  2. Changsha Public Security Bureau, Changsha, 410008, Hunan, People’s Republic of China

    Yong Fu

  3. Department of Oral and Maxillofacial Surgery, Xiangya Stomatological Hospital, Central South University, Changsha, 410008, Hunan, People’s Republic of China

    Ying Liu

  4. Department of Internal Medicine, Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao, 028050, Inner Mongolia Autonomous Region, People’s Republic of China

    Jinshan Liu

Authors
  1. Xiaoliang Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yong Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ying Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Juanjuan Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yanfang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yadong Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jie Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jifeng Cai
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Jinshan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Lagabaiyila Zha
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lagabaiyila Zha.

Ethics declarations

The study was approved by the Ethics Committee of Central South University, Xiangya School of Medicine (Approval Code: 201303147).

Conflict of interest

The authors declare that they have no competing interests.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(XLS 92 kb)

ESM 2

(XLS 27 kb)

ESM 3

(XLS 23 kb)

ESM 4

(XLS 21 kb)

ESM 5

(XLS 12 kb)

ESM 6

(XLS 21 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fu, X., Fu, Y., Liu, Y. et al. Genetic polymorphisms of 26 Y-STR loci in the Mongolian minority from Horqin district, China. Int J Legal Med 130, 941–946 (2016). https://doi.org/10.1007/s00414-016-1387-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00414-016-1387-3

Keywords

Search

Navigation