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Forensic parameters of the Investigator DIPplex kit (Qiagen) in six Mexican populations

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Abstract

Allele frequencies and statistical parameters of forensic efficiency for 30 deletion–insertion polymorphisms (DIPs) were estimated in six Mexican populations. For this purpose, 421 unrelated individuals were analyzed with the Investigator DIPplex kit. The Hardy–Weinberg and linkage equilibrium was demonstrated for this 30-plex system in all six populations. We estimated the combined power of discrimination (PD ≥ 99.999999 %) and combined power of exclusion (PE ≥ 98.632705 %) for this genetic system. A low but significant genetic structure was demonstrated among these six populations by pairwise comparisons and AMOVA (F ST ≥ 0.7054; p ≤ 0.0007), which allows clustering populations in agreement with geographical criteria: Northwest, Center, and Southeast.

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Acknowledgments

We thank the financial support from Qiagen and CONACYT (grant no. 129693) to H-RV.

Conflict of interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Instituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara (CUCiénega-UdeG), Av. Universidad #1115, Col. Paso Blanco, CP 47810, Ocotlán, Jalisco, México

    G. Martínez-Cortés, M. García-Aceves, A. F. Favela-Mendoza & H. Rangel-Villalobos

  2. Instituto de Investigación Biomédica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara, Jalisco, México

    J. F. Muñoz-Valle

  3. Escuela de Biología, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México

    J. S. Velarde-Felix

Authors
  1. G. Martínez-Cortés
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  2. M. García-Aceves
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  3. A. F. Favela-Mendoza
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  4. J. F. Muñoz-Valle
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  5. J. S. Velarde-Felix
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  6. H. Rangel-Villalobos
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Corresponding author

Correspondence to H. Rangel-Villalobos.

Electronic supplementary material

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Figure S1

Multidimensional scaling (MDS) plot based on FST distances between five Mestizo populations and one Amerindian group (Purepechas) from Mexico. Population clusters obtained by pairwise comparisons and AMOVA tests are indicated in ovals. (GIF 57 kb)

High resolution image (TIFF 2037 kb)

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Martínez-Cortés, G., García-Aceves, M., Favela-Mendoza, A.F. et al. Forensic parameters of the Investigator DIPplex kit (Qiagen) in six Mexican populations. Int J Legal Med 130, 683–685 (2016). https://doi.org/10.1007/s00414-015-1242-y

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  • DOI: https://doi.org/10.1007/s00414-015-1242-y

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