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Sex-specific fluorescent labelling of cells for laser microdissection and DNA profiling

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Abstract

Sex-specific isolation of cells from mixtures would greatly facilitate forensic casework. Thus, male and female cell mixtures were marked with a fluorescent X/Y-probe CEP X SpectrumOrange/Y SpectrumGreen DNA probe kit for fluorescence in situ hybridization, and single cells were isolated via laser microdissection (LMD). DNA profiling of LMD isolated, hybridized cells showed usable short tandem repeat profiles for at least 20 cells, which are comparable with results from other studies. To simulate casework samples, the method was also optimized for air-dried samples.

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References

  1. Abdelmoula NB, Amouri A, Portnoi MF, Saad A et al (2004) Cytogenetics and fluorescence in situ hybridization assessment of sex-chromosome mosaicism in Klinefelter’s syndrome. Ann Genet 47:163–175

    PubMed  Google Scholar 

  2. Anslinger K, Mack B, Bayer B, Rolf B, Eisenmenger W (2005) Digoxigenin labelling and laser capture microdissection of male cells. Int J Legal Med 119(6):374–377

    Article  PubMed  CAS  Google Scholar 

  3. Becker I, Becker KF, Röhrl MH, Höfler H (1997) Laser-assisted preparation of single cells from stained histological slides for gene analysis. Histochem Cell Biol 108:447–451

    Article  PubMed  CAS  Google Scholar 

  4. Budimlija ZM, Lechpammer M, Popiolek D, Fogt F, Prinz M, Bieber FR (2005) Forensic applications of laser capture microdissection: use in DNA-based parentage testing and platform validation. Croat Med J 46:549–555

    PubMed  Google Scholar 

  5. Di Martino D, Giuffre G, Staiti N, Simone A, Le Donne M, Saravo L (2004) Laser microdissection and DNA typing of cells from single hair follicles. Forensic Sci Int 146S:155–157

    Article  Google Scholar 

  6. Di Martino D, Giuffre G, Staiti N, Simone A, Le Donne M, Saravo L (2004) Single sperm cell isolation by laser microdissection. Forensic Sci Int 146S:151–153

    Article  Google Scholar 

  7. Egeland T, Dalen I, Mostad PF (2003) Estimating the number of contributors to a DNA profile. Int J Legal Med 117:271–275

    Article  PubMed  Google Scholar 

  8. Elliot K, Hill DS, Lambert C, Burroughes TR, Gill P (2003) Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides. Forensic Sci Int 137:28–36

    Article  Google Scholar 

  9. Fernandez GR, Garcia DS, Costoya S, Pasaro E (2000) Analysis of sex chromosome aneuploidy in 41 patients with Turner syndrome: a study of ‘hidden’ mosaicism. Clin Genet 58:201–208

    Article  Google Scholar 

  10. Hu YQ, Fung WK (2003) Interpreting DNA mixtures with the presence of relatives. Int J Legal Med 117:39–45

    Article  PubMed  Google Scholar 

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Correspondence to K. Anslinger.

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Anslinger, K., Bayer, B., Mack, B. et al. Sex-specific fluorescent labelling of cells for laser microdissection and DNA profiling. Int J Legal Med 121, 54–56 (2007). https://doi.org/10.1007/s00414-005-0065-7

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  • DOI: https://doi.org/10.1007/s00414-005-0065-7

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