Skip to main content
SpringerLink
Log in

Evolutionary conservation of kinetochore protein sequences in plants

  • Original Article
  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract.

The evolutionary conservation of structural/functional kinetochore proteins has been studied on isolated nuclei and pro-/metaphase chromosomes of mono- and dicot plants. The cross-reactivities of antibodies against human CENPC, CENPE and CENPF, and against maize CENPCa with the centromeric regions of mitotic chromosomes of Vicia faba and/or Hordeum vulgare are shown. Putative homologs of the kinetochore protein SKP1 (suppressor of kinetochore protein 1p of yeast) were found in both species and of CBF5p (centromere binding factor 5 of yeast) in barley. Antibodies against synthetic peptides derived from partial sequences encoding these proteins were produced and recognized the centromeric regions on mitotic chromosomes as detected by indirect immunofluorescence.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany, , , , ,

    Rogier ten Hoopen, Renate Manteuffel, Ludmilla Malysheva & Ingo Schubert

  2. Institute of Experimental Botany, 77200 Olomouc, Czech Republic, , , , ,

    Jaroslav Doležel

Authors
  1. Rogier ten Hoopen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Renate Manteuffel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jaroslav Doležel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ludmilla Malysheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ingo Schubert
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

in revised form: 27 July 2000

Electronic Publication

Rights and permissions

Reprints and permissions

About this article

Cite this article

ten Hoopen, R., Manteuffel, R., Doležel, J. et al. Evolutionary conservation of kinetochore protein sequences in plants. Chromosoma 109, 482–489 (2000). https://doi.org/10.1007/s004120000109

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s004120000109

Keywords

Search

Navigation