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Association of IL-4 and ADAM33 Gene Polymorphisms with Asthma in an Indian Population

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Abstract

There are more than 100 candidate genes of asthma located on 23 human chromosomes. Interleukin-4 (IL-4), located on chromosome 5q31, and ADAM33, located on chromosome 20p13, and some single nucleotide polymorphisms (SNPs) of these genes have been shown to be associated with asthma and its manifestations in different populations. The most prominent SNPs of IL-4 and ADAM33 are 589C>T and 400A>G, respectively. There are also controversial reports on the association of these SNPs with asthma. In the present study, we analyzed these two SNPs in 100 patients with asthma and 50 controls through PCR amplification and restriction digestion to evaluate association of these two SNPs with asthma. The nonsignificant differences were observed for the IL-4 promoter polymorphism C589T and the ADAM33 T1 polymorphism between asthmatic patients and controls (P = 0.638 and 0.943, respectively). Our data revealed that there is no association of these SNPs with asthma indicating that other SNPs of these genes or other genes might be involved in the manifestation of asthma.

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Acknowledgment

The authors thank the doctors, patients, and patients’ families in Cheluvamba Hospital and JSS Hospital and in the Allergy, Asthma and Chest Center of Mysore; Dr. S. Pushpa, Principal, Mysore Medical College & Research Institute; Dr. Rajendra Kumar, Cheluvamba Hospital, Mysore Medical College & Research Institute; Dr. S.K. Chaya and Dr. A.K. Prabhakar, JSS Hospital, Mysore; Mr. M. Kareem Khan and Mrs. Sumithra, and also all the members of N. B. Ramachandra’s research group for their valuable help and suggestions. Mahdi Bijanzadeh is grateful to the University of Mysore, Chairman, DOS in Zoology, core group of the Unit on Evolution and Genetics, and Ahwaz University of Medical Sciences, Iran, for providing facilities to perform this work.

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Authors and Affiliations

  1. Human Genetics Laboratory, Department of Studies in Zoology, University of Mysore, Manasagangothri, Mysore, 570 006, India

    Mahdi Bijanzadeh & Nallur B. Ramachandra

  2. J.S.S. Medical College, J.S.S. Hospital, Mysore, India

    P. A. Mahesh & B. S. Jayaraj

  3. Department of Pediatrics, Cheluvamba Hospital, Mysore Medical College & Research Institute, Mysore, India

    R. Savitha Mysore & Pradeep Kumar

  4. Blood Bank, Department of Pathology, K.R. Hospital, Mysore Medical College & Research Institute, Mysore, India

    B. S. Manjunath

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  1. Mahdi Bijanzadeh
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  2. Nallur B. Ramachandra
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  3. P. A. Mahesh
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  4. R. Savitha Mysore
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Correspondence to Nallur B. Ramachandra.

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Bijanzadeh, M., Ramachandra, N.B., Mahesh, P.A. et al. Association of IL-4 and ADAM33 Gene Polymorphisms with Asthma in an Indian Population. Lung 188, 415–422 (2010). https://doi.org/10.1007/s00408-010-9247-2

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