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Caveolin 1 (Cav-1) and actin-related protein 2/3 complex, subunit 1B (ARPC1B) expressions as prognostic indicators for oral squamous cell carcinoma (OSCC)

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Abstract

Caveolin-1 (Cav-1) and Actin-Related Protein 2/3 Complex, Subunit 1B (ARPC1B) have been implicated in various human cancers, yet its role in tumorigenesis remains controversial. Therefore, this study aims to determine the protein expression of these two genes in oral squamous cell carcinomas (OSCCs) and to evaluate the clinical and prognostic impact of these genes in OSCC. Protein expressions of these two genes were determined by immunohistochemistry technique. The association between Cav-1 and ARPC1B with clinico-pathological parameters was evaluated by Chi-square test (or Fisher exact test where appropriate). Correlation between the protein expressions of these 2 genes with survival was analyzed using Kaplan–Meier and Cox regression models. Cav-1 and ARPC1B were found to be significantly over-expressed in OSCC compared to normal oral mucosa (p = 0.002 and p = 0.033, respectively). Low level of ARPC1B protein expression showed a significant correlation with lymph node metastasis (LNM) (p = 0.010) and advanced tumor staging (p = 0.003). Kaplan–Meier survival analyses demonstrated that patients with over-expression of Cav-1 protein were associated with poor prognosis (p = 0.030). Adjusted multivariate Cox regression model revealed that over-expression of Cav-1 remained as an independent significant prognostic factor for OSCC (HRR = 2.700, 95 % CI 1.013–7.198, p = 0.047). This study demonstrated that low-expression of ARPC1B is significantly associated with LNM and advanced tumor staging whereas high expression of Cav-1 can be a prognostic indicator for poor prognosis in OSCC patients.

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Acknowledgement

This study was supported by University of Malaya Research Grant (RP002E-13HTM) and University of Malaya Postgraduate Research Fund (PPPC/C1-2012/DEGC/24). The authors acknowledged the Oral Cancer Research and Coordinating Centre (OCRCC), University of Malaya (UM) for providing tissue and data from the Malaysian Oral Cancer Database and Tissue Bank System (MOCDTBS). The authors also thanked the clinicians and pathologists from Ministry of Health Malaysia for their clinical and pathologic expertise.

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Authors and Affiliations

  1. Department of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of Dentistry, Oral Cancer Research and Coordinating Centre, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Lukman Bin Md Auzair, Vui King Vincent-Chong, Thomas George Kallarakkal, Anand Ramanathan, Chia Ee Lee, Zainal Ariff Abdul Rahman, Siti Mazlipah Ismail & Rosnah Binti Zain

  2. Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia

    Vui King Vincent-Chong, Wan Maria Nabillah Ghani, Thomas George Kallarakkal, Anand Ramanathan, Chia Ee Lee, Zainal Ariff Abdul Rahman, Siti Mazlipah Ismail, Mannil Thomas Abraham & Rosnah Binti Zain

  3. Department of Oral & Maxillofacial Surgery, Hospital Tengku Ampuan Rahimah, Ministry of Health, Klang, Selangor, Malaysia

    Mannil Thomas Abraham

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  1. Lukman Bin Md Auzair
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  2. Vui King Vincent-Chong
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Correspondence to Rosnah Binti Zain.

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Auzair, L.B.M., Vincent-Chong, V.K., Ghani, W.M.N. et al. Caveolin 1 (Cav-1) and actin-related protein 2/3 complex, subunit 1B (ARPC1B) expressions as prognostic indicators for oral squamous cell carcinoma (OSCC). Eur Arch Otorhinolaryngol 273, 1885–1893 (2016). https://doi.org/10.1007/s00405-015-3703-9

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