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Topical application of 5-fluorouracil on attic cholesteatoma results in downregulation of keratinocyte growth factor and reduction of proliferative activity

  • Otology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

To investigate the cell-biological effect of topically applied 5-fluorouracil (5-FU) on middle ear cholesteatoma, 12 attic cholesteatomas were treated with topical application of 5-FU cream, two to five times with an interval of 2 weeks (5-FU group). The control group comprised 65 cholesteatoma that were not treated with 5-FU. All lesions were later excised surgically and processed for immunohistochemical analyses of Ki-67, keratinocyte growth factor (KGF) and its receptor (KGFR). 5-FU significantly reduced the expression of KGF, did not change KGFR expression, and significantly reduced the Ki-67 labeling index, relative to the control group. The effect of 5-FU on cholesteatoma seems to be mediated, at least in part, through downregulation of KGF in stromal cells and reduction of the proliferative activity of epithelial cells.

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Authors and Affiliations

  1. Department of Otolaryngology-Head and Neck Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan

    Tomomi Yamamoto-Fukuda, Mariko Terakado & Haruo Takahashi

  2. Department of Histology and Cell Biology, Unit of Basic Medical Science, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, Japan

    Yoshitaka Hishikawa & Takehiko Koji

Authors
  1. Tomomi Yamamoto-Fukuda
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  2. Mariko Terakado
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  3. Yoshitaka Hishikawa
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  4. Takehiko Koji
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  5. Haruo Takahashi
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Corresponding author

Correspondence to Tomomi Yamamoto-Fukuda.

Additional information

This paper was presented at the 9th international symposium on recent advances in otitis media, St. Pete Beach, Florida, USA, 3-7 June 2007.

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Yamamoto-Fukuda, T., Terakado, M., Hishikawa, Y. et al. Topical application of 5-fluorouracil on attic cholesteatoma results in downregulation of keratinocyte growth factor and reduction of proliferative activity. Eur Arch Otorhinolaryngol 265, 1173–1178 (2008). https://doi.org/10.1007/s00405-008-0597-9

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  • DOI: https://doi.org/10.1007/s00405-008-0597-9

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