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The effect of hesperetin on ischemia–reperfusion injury in rat ovary

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
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Abstract

Purpose

Hesperidin (HES), a citrus fruit extract, has beneficial effects on various ischemia/reperfusion (I/R) models. We aimed to evaluate the possible positive effects of hesperetin (HPT), an active metabolite of HES, on a rat ovarian I/R model.

Methods

We divided 24 Wistar Albino rats into four groups. Group I (n = 6) was sham operated, Group II (n = 6) was the I/R group, Group III (n = 6) was the I/R + solvent group and Group IV (n = 6) was the I/R + HPT group. Three hours of ischemia and 3 h of reperfusion were performed on each rat in Groups II, III, and IV. Dimethyl sulfoxide (DMSO) was given intraperitoneally to the rats in the III. Group, and 50 mg/kg of HPT dissolved in DMSO was given intraperitoneally to the rats in the IV. Group 30 min before reperfusion. After 3 h of reperfusion, the ipsilateral ovaries of the rats were examined immunohistochemically to detect apoptosis.

Results

Hematoxylin and eosin (H and E) staining demonstrated less edema and hemorrhage in the group where HPT was applied. Caspase-3 and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining showed significantly lower apoptosis in the group where HPT was used when compared to either the I/R or solvent group.

Conclusions

To the best of our knowledge, this is the first study that shows the beneficial effects of HPT in an ovarian I/R injury. HPT improved tissue damage and apoptosis caused by I/R injury. To identify the possible positive effects of HPT in ovarian torsion of humans and use in clinical practice, more studies must be performed.

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Acknowledgments

We are indebted to the staff of the Experimental Research Center of Canakkale Onsekiz Mart University. Disclosure none.

Conflict of interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

    Ayse Nur Cakir Gungor, Meryem Gencer, Servet Hacivelioglu, Ahmet Uysal & Emine Cosar

  2. Department of Histology and Embryology, Faculty of Medicine, Edirne Trakya University, Edirne, Turkey

    Turan Karaca & Selim Demirtas

  3. Obstetrics and Gynecology Clinic, Beykoz Government Hospital, Istanbul, Turkey

    Fatma Korkmaz

Authors
  1. Ayse Nur Cakir Gungor
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  2. Meryem Gencer
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Correspondence to Ayse Nur Cakir Gungor.

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Cakir Gungor, A.N., Gencer, M., Karaca, T. et al. The effect of hesperetin on ischemia–reperfusion injury in rat ovary. Arch Gynecol Obstet 290, 763–769 (2014). https://doi.org/10.1007/s00404-014-3267-8

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  • DOI: https://doi.org/10.1007/s00404-014-3267-8

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