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Molecular characterization of early adenocarcinoma of the uterine cervix by oligonucleotide microarray

  • Gynecologic Oncology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

In an attempt to understand the molecular characterization for the early adenocarcinoma of the uterine cervix, an analysis of gene expression profiles obtained from early adenocarcinoma of the uterine cervix was performed to find those genes most aberrantly expressed.

Methods and materials

Total RNA was prepared from 32 samples of early adenocarcinoma of the uterine cervix and 32 paired normal cervix tissues, and hybridized to cancer-associated oligonucleotide microarrays with probe sets complementary to about 1,426 transcripts.

Results

Supervised analysis of gene expression data identified 13 genes that exhibited >2-fold upregulation and 27 genes >2-fold downregulation, respectively, in early adenocarcinoma of the uterine cervix compared to normal cervix. Unsupervised hierarchical clustering of the expression data readily distinguished early adenocarcinoma of the uterine cervix from normal cervix. Two genes (karyopherin alpha 2 and proliferating cell nuclear antigen) were selected randomly for real-time reverse transcription polymerase chain reaction analysis. Both genes were expressed significantly higher in early adenocarcinoma of the uterine cervix than in normal cervix, with p = 0.0003 and <0.0001, respectively. These results were compatible with the microarray data.

Conclusions

This study has revealed several genes that may be highly attractive candidate molecular markers/targets for early adenocarcinoma of the uterine cervix.

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Acknowledgments

This study was supported by the Guangdong Provincial Scientific Research Item (Grant 2004B30301007) and partially supported by Research Fund of State Key Laboratory of Oncology in Southern China.

Conflict of interest statement

The authors declare that there are no conflicts of interest.

Author information

Authors and Affiliations

  1. Department of Radiology, First Affiliated Hospital, Medical College of Shantou University, Shantou, 515041, China

    Ling-Min Liao

  2. Guangdong Key Laboratory of Medical Molecular Imaging, Shantou, 515041, China

    Ling-Min Liao

  3. State Key Laboratory of Oncology in Southern China, 651 Dongfeng Road East, Guangzhou, 510060, China

    Min Zheng, Long Huang, Hui Ding, Li He & Hui-Yun Wang

  4. Department of Gynecology, Cancer Center, Sun Yat-sen University, 651 Dongfeng Road East, Guangzhou, 510060, Guangdong, China

    Min Zheng, Long Huang, Hui Ding & Li He

  5. Department of Sample Bank, Cancer Center, Sun Yat-sen University, Guangzhou, 510060, China

    Ying Zhang

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  1. Ling-Min Liao
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Correspondence to Min Zheng.

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Liao, LM., Zheng, M., Huang, L. et al. Molecular characterization of early adenocarcinoma of the uterine cervix by oligonucleotide microarray. Arch Gynecol Obstet 283, 861–869 (2011). https://doi.org/10.1007/s00404-010-1511-4

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  • DOI: https://doi.org/10.1007/s00404-010-1511-4

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