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Altered gene profile of placenta from women with intrahepatic cholestasis of pregnancy

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Abstract

Objectives

To investigate the alterations in gene profile of placenta from pregnant women with intrahepatic cholestasis of pregnancy (ICP) and to enhance the insight of etiology and pathogenesis of ICP.

Methods

Ten pregnant women diagnosed ICP were recruited and 10 healthy pregnant women served as control. Four samples were taken from each placenta and RNA was isolated. Gene expression was analyzed with microarray and real time PCR was used to validate the differentially expressed genes.

Results

392 genes were found differentially expressed. Among these differentially expressed genes, 280 were up-regulated and 112 were down-regulated. These differentially expressed genes involved 20 categories including genes involved in transportation, cell growth, apoptosis and immune response that were putatively participated the pathogenesis of ICP.

Conclusions

293 differentially expressed genes of 20 categories were found in ICP placenta, suggesting the diversity of gene expression alteration and the complexity of etiology and pathogenesis of ICP.

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Acknowledgment

This study was financially supported by ZJNSF (No Y205297).

Author information

Author notes

  1. Jing Wei

    Present address: Tongde Hospital of Zhejiang Province, Hangzhou, China

Authors and Affiliations

  1. Women’s Hospital, School of Medicine, Zhejiang University, Add: 2 Xueshi Road, 310006, Hangzhou, China

    Jing Wei, Huimin Wang, Xiaofu Yang, Minyue Dong & Zhengping Wang

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  1. Jing Wei
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  2. Huimin Wang
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  3. Xiaofu Yang
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  4. Minyue Dong
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  5. Zhengping Wang
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Corresponding author

Correspondence to Zhengping Wang.

Additional information

J. Wei and H. Wang were contributed equally.

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Wei, J., Wang, H., Yang, X. et al. Altered gene profile of placenta from women with intrahepatic cholestasis of pregnancy. Arch Gynecol Obstet 281, 801–810 (2010). https://doi.org/10.1007/s00404-009-1156-3

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  • DOI: https://doi.org/10.1007/s00404-009-1156-3

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