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Purified proteins from leishmania amastigotes-induced delayed type hypersensitivity reactions and remission of collagen-induced arthritis in animal models

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Abstract

A treatment preparation composed of purified Leishmania (L) antigenic fractions (AS210) induced linear delayed type hypersensitivity (DTH) reactions over a 1–40 μg dose range, in guinea pigs. When a DBA-1 mouse collagen induced arthritis (CIA) model was used to compare AS210 treatment against: a polyvalent vaccine (AS110-1), a monovalent vaccine (AS110-2) and placebo, the AS210 treated mice had the least amount of forepaw inflammation and the lowest mean arthritis scores (MAS). When MAS for day(s) 1–40 were analyzed using one way ANOVA, statistically significant (P < 0.05) differences were seen for the following study groups: PBS versus Dexamethasone and PBS versus AS210. Subsequently, the ANOVA analysis results were corroborated by the Mann–Whitney test: analysis of the first group (P < 0.001) and analysis of the second group (P < 0.001). Comparison between dexamethasone and AS210 at different time intervals by Mann–Whitney test were as follows: day 0–day 5 both treatments had equal values (P = 1.00), from day-7 to 20 AS210 treatment had lower MAS values than dexamethasone (P = 0.037), and from day-21 to 30, AS210 MAS were similar to dexamethasone values (P = 0.319). No statistical difference was observed between AS110-1, AS110-2, and placebo groups.

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Acknowledgments

We would like to thank Jose Godoy for critical discussions, suggesting useful ideas and his excellent help as Medical Writer. Jose A. O’Daly, Astralis CSO, CEO and Chairman, would also like to thank The Technology Business Tax Certificate Transfer Program of the Greater State of New Jersey, Economic Development Authority (EDA). This study was realized in accordance with Home Office MD Biosciences Inflammation Discovery Services, Ltd, UK animal guidelines, and is performed following an application-form review by the Committee for Ethical Conduct in the Care and Use of Laboratory Animals. The study complies with the rules and regulations set forth.

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  1. Astralis LTD., 75 Passaic Ave., Fairfield, NJ, 07004, USA

    Jose Antonio O’Daly, J. P. Gleason, G. Peña & I. Colorado

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  1. Jose Antonio O’Daly
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Correspondence to Jose Antonio O’Daly.

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O’Daly, J.A., Gleason, J.P., Peña, G. et al. Purified proteins from leishmania amastigotes-induced delayed type hypersensitivity reactions and remission of collagen-induced arthritis in animal models. Arch Dermatol Res 302, 567–581 (2010). https://doi.org/10.1007/s00403-009-1026-7

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  • DOI: https://doi.org/10.1007/s00403-009-1026-7

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