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rhBMP-2 not alendronate combined with HA-TCP biomaterial and distraction osteogenesis enhance bone formation

  • Orthopaedic Surgery
  • Published:
Archives of Orthopaedic and Trauma Surgery Aims and scope Submit manuscript

Abstract

Introduction

The long treatment duration of distraction osteogenesis (DO) usually causes some complications such as re-fracture, non-union. We have previously demonstrated that the combined use of biomaterial with distraction osteogenesis technique can enhance bone formation and consolidation. This study further tested whether the use of biological agents such as rhBMP-2 or alendronate together with biomaterials in DO will further promote bone formation.

Methods

A 1.0-cm tibial shaft was removed in the left tibia of 30 rabbits. The 1.0-cm defect gap was reduced to 0.5 cm and the remaining 0.5-cm defect gap was filled with 0.5-cm restorable hydroxyapatite/tri-calcium phosphates (HA/TCP) cylindrical block. The animals were divided into three groups with the following added on the HA/TCP block: Group A 50 μl of saline, Group B 75 μg rhBMP-2 in 50 μl of saline, Group C 250 μg alendronate in 50 μl saline. The tibia was then fixed with unilateral lengthener and lengthening started 7 days after at a rate of 1.0 mm/day for 5 days. All animals were terminated at day 37 following surgery. The excised bone specimens were subject to micro-CT, mechanical testing and histological examinations.

Results

Bone mineral density and content were significantly higher in Groups A and B compared to Group C and the mechanical properties of the regenerates in Group B were highest. Micro-CT and histological examinations also confirmed that the regenerates in Group B had the most advanced bone formation, consolidation and remodeling comparing to other groups.

Conclusion

The combined use of rhBMP-2 with HA-TCP biomaterial in DO has significantly enhanced bone formation and consolidation than using the HA-TCP biomaterials alone, whereas the use of alendronate has inhibitory effects on bone formation.

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Acknowledgments

We thank Mr. Jason Hendry, Millenium Biologix Corporation, Kingston, Canada for providing the cylindrical Skelite™ scaffolds for this study.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, General Hospital of Chinese People’s Liberation Army, Beijing, 100853, People’s Republic of China

    Ming Ni, Pei-Fu Tang & Yan Wang

  2. Department of Orthopaedics and Traumatology, Faculty of Medicine, The Chinese University Hong Kong, Clinical Sciences Building, Prince of Wales Hospital, Shatin, Hong Kong, People’s Republic of China

    Ming Ni, Gang Li & Kai-Ming Chan

  3. Stem Cells and Regenerative Program, School of Biomedical Sciences, The Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong, People’s Republic of China

    Ming Ni & Gang Li

  4. CUHK-WHO Collaborating Centre for Sports Medicine and Health Promotion, The Chinese University Hong Kong, Clinical Sciences Building, Prince of Wales Hospital, Shatin, Hong Kong, People’s Republic of China

    Gang Li & Kai-Ming Chan

Authors
  1. Ming Ni
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  2. Gang Li
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  3. Pei-Fu Tang
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  4. Kai-Ming Chan
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  5. Yan Wang
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Corresponding authors

Correspondence to Gang Li or Yan Wang.

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Ni, M., Li, G., Tang, PF. et al. rhBMP-2 not alendronate combined with HA-TCP biomaterial and distraction osteogenesis enhance bone formation. Arch Orthop Trauma Surg 131, 1469–1476 (2011). https://doi.org/10.1007/s00402-011-1357-7

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  • DOI: https://doi.org/10.1007/s00402-011-1357-7

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