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Preparation and characterization of cross-linked hyaluronan nanoparticles

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Abstract

The present investigation describes the synthesis and characterization of novel biodegradable nanoparticles based on hyaluronic acid (HA). The diamine, 2,2′(ethylenedioxy)bis(ethylamine) was used for cross-linking of the HA linear chains. The condensation reaction of amino groups and pendant carboxyl groups of HA was performed in aqueous media at room temperature using water-soluble carbodiimide. The prepared nanosystems, aqueous solutions, or dispersions of nanoparticles were stable, transparent, or mildly opalescent systems depending on the ratio of cross-linking, findings consistent with values of transmittance above 77%. The structure of products was determined by nuclear magnetic resonance spectroscopy, and the particle size was identified by laser light scattering (DLS) and transmission electron microscopy (TEM) measurements. Particle size measured by TEM varied less than 130 nm; in the swollen state, the average size of the particles measured by DLS was in the range of 30–140 nm depending on the ratio of cross-linking and the molecular weight of HA. Formation of cross-linked nanoparticles results in a viscosity drop compared to the viscosity of the corresponding solution of the HA, and this trend becomes decreasingly appreciable as the molecular weight of HA decreases.

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Acknowledgements

This work was supported by RET (Grant of Regional University Knowledge Center) contract number (RET-06/432/2004) and by ElizaNor Polymer LLC, USA.

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Authors and Affiliations

  1. Solid State Physics, University of Debrecen, 4010, Debrecen, Hungary

    Magdolna Bodnár & Lajos Daróczi

  2. Department of Biochemistry, University of Debrecen, 4010, Debrecen, Hungary

    Gyula Batta

  3. Department of Prosthetic Dentistry, University of Debrecen, 4010, Debrecen, Hungary

    József Bakó

  4. ElizaNor Polymer LLC, Princeton Junction, NJ, 08550, USA

    John F. Hartmann

  5. BBS Nanotechnology Ltd., 4225, Debrecen, P.O. Box 12, Hungary

    Magdolna Bodnár & János Borbély

Authors
  1. Magdolna Bodnár
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  2. Lajos Daróczi
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  3. Gyula Batta
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  4. József Bakó
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  5. John F. Hartmann
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  6. János Borbély
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Correspondence to János Borbély.

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Bodnár, M., Daróczi, L., Batta, G. et al. Preparation and characterization of cross-linked hyaluronan nanoparticles. Colloid Polym Sci 287, 991–1000 (2009). https://doi.org/10.1007/s00396-009-2061-9

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  • DOI: https://doi.org/10.1007/s00396-009-2061-9

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