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Octreotide alleviates obesity by reducing intestinal glucose absorption and inhibiting low-grade inflammation

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Abstract

Purpose

To investigate the role of octreotide, a somatostatin (SST) analog with anti-inflammatory effects, on the digestive and absorptive functions of jejunum in rats fed a high-fat diet, as well as its therapeutic prospects for diet-induced obesity.

Methods

Rats were divided into three groups with different diet and treatment for the 176-day experiment: (1) control, 18 rats fed with standard chow, (2) high-fat control, 19 rats fed with high-fat chow, and (3) high-fat octreotide, 21 rats fed with high-fat chow and treated with octreotide for the last 8 days of the experiment. Plasma tumor necrosis factor-α (TNF-α) was measured by ELISA and SST by radioimmunoassay. Disaccharidase activity in the jejunal homogenate was determined. SST and Na+-dependent glucose transporter 1 (SGLT-1) in the jejunal mucosa were visualized by immunohistochemistry. SGLT-1 was quantified by reverse transcription polymerase chain reaction and Western blot assays.

Results

After 176 days, the fat/body weight ratio, villus height, maltase, SGLT-1, and plasma TNF-α in the high-fat control rats were much higher than those in the control rats (p < 0.01 or p < 0.05) and were significantly lower in the high-fat + octreotide rats (p < 0.01 or p < 0.05). SST levels were dramatically different in the intestinal mucosa of the two high-fat groups (231.12 ± 98.18 pg/mg in the high-fat controls and 480.01 ± 286.65 pg/mg in the octreotide group).

Conclusions

The low-grade inflammation induced by high-fat diet apparently reduced the secretion of intestinal SST, which increased intestinal absorption of energy and nutrients and formation of adipose tissues. Octreotide effectively reversed this process, a finding that has far-reaching significance for the regulation of energy balance.

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Acknowledgments

This work was supported by grants from the National Natural Sciences Foundation of China (No. 30870919) and Sichuan Provincial Department of Science and Technology (No. 2010SZ0176).

Conflict of interest

The authors declared no conflict of interest.

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Authors and Affiliations

  1. Division of Peptides Related with Human Disease, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    R. Liu, N. Wei, W. Guo, O. Qiang, X. Li, Y. Ou, W. Huang & C. W. Tang

  2. Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    N. Wei, Y. Ou, W. Huang & C. W. Tang

Authors
  1. R. Liu
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  2. N. Wei
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  3. W. Guo
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  4. O. Qiang
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  5. X. Li
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  6. Y. Ou
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  7. W. Huang
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  8. C. W. Tang
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Correspondence to R. Liu or C. W. Tang.

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Liu, R., Wei, N., Guo, W. et al. Octreotide alleviates obesity by reducing intestinal glucose absorption and inhibiting low-grade inflammation. Eur J Nutr 52, 1067–1075 (2013). https://doi.org/10.1007/s00394-012-0413-6

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  • DOI: https://doi.org/10.1007/s00394-012-0413-6

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