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Protective effects of caffeic acid phenethyl ester (CAPE) on intestinal damage in necrotizing enterocolitis

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Abstract

Objective

To determine the preventative effect of caffeic acid phenethyl ester (CAPE) in necrotizing enterocolitis (NEC) in an experimental rat model of NEC.

Materials and methods

Thirty newborn Sprague–Dawley rats were randomly divided into three groups; as NEC, NEC + CAPE and control. NEC was induced by enteral formula feeding, subjected to hypoxia–hyperoxia and cold stress. Pups in the NEC + CAPE group were treated with CAPE at a dose of 30 mg/kg daily by intraperitoneal route from the first day to the end of the study. All pups were executed on the fourth day. Proximal colon and ileum were allocated for histopathologic and biochemical evaluation, including xanthine oxidase (XO), total antioxidant status (TAS), total oxidant status (TOS), malonaldehyde (MDA) and myeloperoxidase (MPO) activities.

Results

The pups in the NEC + CAPE group had better histopathologic and apoptosis evaluations (TUNEL and caspase-9) and the severity of bowel damage was significantly lower in the NEC + CAPE group compared to the NEC group (P < 0.01). The clinical sickness scores and body weight in the NEC + CAPE group was significantly better compared to the NEC group (P < 0.05). Tissue MDA, MPO, XO levels and TOS were remarkably reduced in the NEC + CAPE group, however, TAS was significantly increased in the NEC + CAPE group (P < 0.05).

Conclusion

Treatment with CAPE reduces the intestinal damage in NEC.

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Acknowledgments

This study was supported by a Grant of Fatih University Scientific Research Committee.

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Authors and Affiliations

  1. Department of Neonatology, Fatih University Faculty of Medicine, 06510, Ankara, Turkey

    Cuneyt Tayman, Alparslan Tonbul & Mustafa Mansur Tatli

  2. Department of Obstetric and Gynecology, Fatih University Faculty of Medicine, 06510, Ankara, Turkey

    Aydın Kosus

  3. Department of Neonatology, Gazi University Faculty of Medicine, 06510, Ankara, Turkey

    Ibrahim Murat Hirfanoglu

  4. Department of Pathology, Fatih University Faculty of Medicine, 06510, Ankara, Turkey

    Hacer Haltas

  5. Department of Biochemystry, Fatih University Faculty of Medicine, 06510, Ankara, Turkey

    Sema Uysal

  6. Department of Pediatric Surgery, Fatih University Faculty of Medicine, 06510, Ankara, Turkey

    Fatih Andiran

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  1. Cuneyt Tayman
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Correspondence to Cuneyt Tayman.

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Tayman, C., Tonbul, A., Kosus, A. et al. Protective effects of caffeic acid phenethyl ester (CAPE) on intestinal damage in necrotizing enterocolitis. Pediatr Surg Int 27, 1179–1189 (2011). https://doi.org/10.1007/s00383-011-2942-0

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