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Reduction of peritoneal adhesions by sustained and local administration of epidermal growth factor

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Abstract

Previous studies have shown epidermal growth factor (EGF) facilitate peritoneal membrane healing by augmenting cell adhesion and migration. The objective of this study was to show the effect of sustained and local administration of EGF on peritoneal adhesion. Fourty-two rats were divided into six groups: control 7 and 14, gelatin 7 and 14, and EGF 7 and 14. Adhesions were created by scraping the cecum with mesh gause followed by application of absolute alcohol and placement of silk suture in the parietal peritoneum. The anterior walls of the intestines were covered with 5 × 5 cm unloaded, and EGF loaded gelatin films in the gelatin and EGF groups, respectively. The rats were killed on days 7 and 14 to assess the adhesion occurring, and for biochemical examination. The mean adhesion grades of EGF groups were significantly lower than in the other groups (P < 0.008). The mean adenosine deaminase (ADA) measurements of EGF 7 group were lower than in the gelatin 7 and control 7 groups but the difference was not significant (P > 0.008). The mean ADA measurements in the 14 days groups were as follows: control 14 < EGF 14 < gelatin 14 groups. The mean ADA measurements between 14 days groups did not significantly differ from each other (P > 0.008). The mean hydroxyproline measurements did not differ among the groups (P > 0.008). EGF decreased intestinal adhesion in our study. EGF has important roles in DNA synthesis and cell proliferation. Further studies are required to determine the exact mechanism by which EGF lowers the efficiency of intestinal adhesion.

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Authors and Affiliations

  1. Department of Pediatric Surgery, Turgut Ozal Medical Center, Faculty of Medicine, Inonu University, 44069, Malatya, Turkey

    S. Uguralp, M. Akin & B. Harma

  2. Department of Biochemistry, Inonu University, Malatya, Turkey

    A. Bay Karabulut & T. R. Kıran

  3. Department of Chemistry, Faculty of Art and Sciences, Middle East Technical University, Ankara, Turkey

    Aysel Kiziltay & N. Hasirci

Authors
  1. S. Uguralp
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  2. M. Akin
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  3. A. Bay Karabulut
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  4. B. Harma
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  5. Aysel Kiziltay
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  6. T. R. Kıran
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  7. N. Hasirci
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Correspondence to S. Uguralp.

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Uguralp, S., Akin, M., Karabulut, A.B. et al. Reduction of peritoneal adhesions by sustained and local administration of epidermal growth factor. Pediatr Surg Int 24, 191–197 (2008). https://doi.org/10.1007/s00383-007-2059-7

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  • DOI: https://doi.org/10.1007/s00383-007-2059-7

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