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Establishment and characterization of xenograft models of human neuroblastoma bone metastasis

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Abstract

Objects

To improve the therapy of advanced neuroblastoma (NB), it is critical to develop animal models that mimic NB bone metastases. Unlike the human disease, NB xenograft models rarely metastasize spontaneously to bone from the orthotopic site of primary tumor growth.

Methods

Single-cell suspensions of SY5Y, KCNR NB cells were injected directly into the femur of nude mice. Radiological and histological analyses and immunohistochemistry analyses were performed to characterize these osseous NB models. SY5Y and KCNR result in osteolytic responses.

Results

We have detected osteoprotegerin, receptor activator of nuclear factor kappa B ligand, parathyroid hormone-related protein, and endothelin-1, proteins associated with bone growth and osteolysis, and C-X-C chemokine receptor type 4 (CXCR4) involved in tumor growth and tumor cell migration in the NB cells grown in the bone.

Conclusions

These animal models can be used to study biological interactions, pathways, and potential therapeutic targets and also to evaluate new agents for treatment and prevention of NB bone metastasis.

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Acknowledgments

This work was supported by National Natural Science Foundation grant from China National Science Foundation Committee (project code 81172410).

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Authors and Affiliations

  1. Department of Neurosurgery, Shengjing Hospital, China Medical University, Shenyang, China

    Hongyu Zhao, Shuai Li, Zuke Da, Liang Ma, Yaoxin Lin & Debao Zhi

  2. Department of Oncology, Shengjing Hospital, China Medical University, Shenyang, China

    Weisong Cai

  3. Department of Pathology, Shengjing Hospital, China Medical University, Shenyang, China

    Hanxue Sun

Authors
  1. Hongyu Zhao
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  2. Weisong Cai
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  3. Shuai Li
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  4. Zuke Da
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  5. Hanxue Sun
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  6. Liang Ma
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  7. Yaoxin Lin
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  8. Debao Zhi
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Corresponding author

Correspondence to Hongyu Zhao.

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Zhao, H., Cai, W., Li, S. et al. Establishment and characterization of xenograft models of human neuroblastoma bone metastasis. Childs Nerv Syst 28, 2047–2054 (2012). https://doi.org/10.1007/s00381-012-1909-3

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  • DOI: https://doi.org/10.1007/s00381-012-1909-3

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