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Hsp90 and its co-chaperone, Sgt1, as autoantigens in dilated cardiomyopathy

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Abstract

Recently, it has been suggested that some heat shock proteins such as Hsp70 and Hsp60 are involved in autoimmune diseases including cardiospecific ones. In this work we focused on the involvement of another well known heat shock protein, Hsp90, and its novel co-chaperone, Sgt1, in dilated cardiomyopathy (DCM). We found that the level of autoantibodies against these two proteins was significantly higher in patients with DCM and ischemic heart disease than in sera of healthy donors. We have also analyzed the expression level and subcellular localization of Hsp90 and Sgt1 in diseased myocardia. Using Western blot we found changes in subcellular localization of Hsp90 in the left ventricle of DCM hearts while the total level of this protein remained unchanged. Regarding the Sgt1 protein, we found an increased level in DCM and no changes in subcellular localization. Taken together, our data suggest that Hsp90 and Sgt1 might be involved in the progression of heart failure and might serve as markers for cardiomyopathies of different origin.

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Abbreviations

DCM:

Dilated cardiomyopathy

IHD:

Ischemic heart disease

HD:

Healthy donors

NYHA:

New York Heart Association diagnostic criteria in cardiovascular diseases

Hsp:

Heat shock protein

SDS:

Sodium dodecyl sulfate

WHO:

World Health Organization

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Acknowledgments

We thank Professor J. Kuznicki, International Institute of Molecular and Cell Biology in Warsaw, for support and advice. Also, we thank Dr M. Żabka for providing the recombinant Sgt1 protein. This work was supported by the Joint Ukrainian-Polish grant NM\134-2009 from the Ministry of Education and Science of Ukraine, and by statutory funds from the Nencki Institute of Experimental Biology.

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Authors and Affiliations

  1. Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv, Ukraine

    Lyudmila L. Kapustian, Olga A. Vigontina, Olga T. Rozhko, Irina V. Kroupskaya & Lyudmila L. Sidorik

  2. Strazhesko Institute of Cardiology MAS of Ukraine, Kyiv, Ukraine

    Dmytro V. Ryabenko

  3. Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093, Warsaw, Poland

    Wojciech Michowski, Wiesława Lesniak & Anna Filipek

  4. International Institute of Molecular Biology, Warsaw, Poland

    Wojciech Michowski

Authors
  1. Lyudmila L. Kapustian
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  2. Olga A. Vigontina
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  3. Olga T. Rozhko
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  4. Dmytro V. Ryabenko
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  5. Wojciech Michowski
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  7. Anna Filipek
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  8. Irina V. Kroupskaya
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  9. Lyudmila L. Sidorik
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Correspondence to Anna Filipek or Lyudmila L. Sidorik.

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Kapustian, L.L., Vigontina, O.A., Rozhko, O.T. et al. Hsp90 and its co-chaperone, Sgt1, as autoantigens in dilated cardiomyopathy. Heart Vessels 28, 114–119 (2013). https://doi.org/10.1007/s00380-011-0226-1

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