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Expression of heat shock proteins and nitrotyrosine in small arteries from patients with coronary heart disease

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Abstract

Heat shock proteins (HSPs) have been suggested to play an important role in the pathogenesis of cardiovascular disease; however, their levels in resistance arteries and their role as useful markers for endothelial dysfunction are not well known. In this paper we studied the levels of HSP90, HSP70, HSP60, HSP27, and of the oxidative stress marker nitrotyrosine (NT) in isolated small subcutaneous arteries from female and male patients with coronary heart disease (CHD) and compared them with healthy controls. HSPs and NT levels were analyzed by immunohistochemistry (IHC) with streptavidin-biotin complex and 3,3′-diaminobenzidine (DAB) staining. The results were assessed with a semi-quantitative method. The study showed lower levels of HSP90 in arteries from both male and female patients when compared to the healthy controls, while levels of HSP70 were lower only in male patients versus controls. The levels of HSP60 and HSP27 did not show any significant difference in either the male or the female groups. NT levels were higher in the arteries from female patients as compared to controls. In conclusion, the present study strengthens the concept that HSPs may play an important role in the pathogenesis of CHD, and that at least two of them, HSP70 and HSP90, may have useful applications as markers of vascular dysfunction in resistance arteries.

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Authors and Affiliations

  1. Institution for Clinical Science Intervention and Technology (CLINTEC), Division of Obstetrics and Gynecology, Karolinska Institutet, Karolinska University Hospital, Huddinge Campus, 14186, Stockholm, Sweden

    Anton Paier & Karolina Kublickiene

  2. Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia

    Anton Paier

  3. Department of Cardiology, Karolinska Institutet, Karolinska University Hospital, Huddinge Campus, 14186, Stockholm, Sweden

    Stefan Agewall

  4. Cardiology Division, Department of Medicine, Aker University Hospital and Oslo University, Oslo, Norway

    Stefan Agewall

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  1. Anton Paier
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  2. Stefan Agewall
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  3. Karolina Kublickiene
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Correspondence to Karolina Kublickiene.

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Paier, A., Agewall, S. & Kublickiene, K. Expression of heat shock proteins and nitrotyrosine in small arteries from patients with coronary heart disease. Heart Vessels 24, 260–266 (2009). https://doi.org/10.1007/s00380-008-1117-y

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  • DOI: https://doi.org/10.1007/s00380-008-1117-y

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