Abstract
Biogeochemical factors responsible of the highly variable content of soil organic matter (SOM) in the different types of soils are poorly known. In particular, the role of organo-mineral interactions has frequently been considered, but less attention has been paid to the molecular composition of the SOM. The aim of this work was to contribute to a better qualitative and quantitative assessment of the soil organic C (SOC) accumulation, using chemometric approaches that do not require the absolute knowledge of the structure and functioning of the whole system under study. For this reason, we monitored the n-alkanes released by analytical pyrolysis from 35 widely different Mediterranean soils. The H′ Shannon diversity index was calculated to evaluate the origin and transformations of the alkane homologous series (C9–C31). A series of multivariate data treatments succeeded in showing significant relationship between the diversity of alkanes and the SOC concentration, and additional indicators of SOM quality were also used. All statistical analyses pointed out the significant correlation (P < 0.01) between the H′ diversity of the pyrolytic alkanes and the amount of SOC. In particular, a significant relationship between SOC levels and the percentage of long-chain alkanes was found, whereas the percentage of short-chain alkanes was correlated with specific descriptors of SOM quality. Finally, the partial least squares (PLS) predicted the SOC content from the alkane patterns.
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References
Almendros G, Dorado J (1999) Molecular characteristics related to the biodegradability of humic acid preparations. Eur J Soil Sci 50:227–236. https://doi.org/10.1046/j.1365-2389.1999.00240.x
Almendros G, Sanz J, González-Vila FJ, Martín F (1991) Evidence for a polyalkyl nature of soil humin. Naturwissenschaften 78:359–362. https://doi.org/10.1007/BF01131609
Almendros G, Hernández Z, Sanz J, Rodríguez-Sánchez S, Jiménez-González MA, González-Pérez JA (2017) Graphical statistical approach to soil organic matter resilience using analytical pyrolysis data. J Chromatogr A 1533:164–173. https://doi.org/10.1016/j.chroma.2017.12.015
Amblès A, Jacquesy JC, Jambu P, Joffre J, Maggi-Churin R (1991) Polar lipid fraction in soil: a kerogen-like matter. Org Geochem 17:341–349. https://doi.org/10.1016/0146-6380(91)90097-4
Baldock JA, Oades JM, Waters AG, Peng X, Vassallo AM, Wilson MA (1992) Aspects of the chemical structure of soil organic materials as revealed by solid-state 13C NMR spectroscopy. Biogeochemistry 16:1–42. https://doi.org/10.1007/BF02402261
Banerjee S, Kirkby CA, Schmutter D, Bissett A, Kirkegaard JA, Richardson AE (2016) Network analysis reveals functional redundancy and keystone taxa amongst bacterial and fungal communities during organic matter decomposition in an arable soil. Soil Biol Biochem 97:188–198. https://doi.org/10.1016/j.soilbio.2016.03.017
Blake GR, Hartge KH (1986) Particle density. In: Klute (ed) Methods of soil analysis: part 1—physical and mineralogical methods. American Society of Agronomy and Soil Science Society of America, Madison, pp 363–375
Bouyoucos GJ (1927) The hydrometer as a new method for the mechanical analysis of soils. Soil Sci 23:343–353
Dabin B (1976) Méthode d’extraction et de fractionnement des matières humiques du sol: application à quelques études pédologiques et agronomiques dans les sols tropicaux. Cah ORSTOM ser Pédol 14:287–297
De la Rosa JM, González-Pérez JA, González-Vázquez R, Knicker H, López-Capel E, Manning DAC, González-Vila FJ (2008) Use of pyrolysis/GC-MS combined with thermal analysis to monitor C and N changes in soil organic matter from Mediterranean fire affected forest. Catena 74:296–303. https://doi.org/10.1016/j.catena.2008.03.004
Dinel H, Lévesque M, Mehuys GR (1991) Effects of long-chain aliphatic compounds on the aggregate stability of a lacustrine silty clay. Soil Sci 151:228–239. https://doi.org/10.1097/00010694-199103000-00005
Duan Y, He Y (2011) Distribution and isotopic composition of n-alkanes from grass, reed and tree leaves along a latitudinal gradient in China. Geochem J 45:199–207. https://doi.org/10.2343/geochemj.1.0115
Duchaufour P, Jacquin F (1975) Comparaison des processus d’humification dans les principaux types d’humus forestiers. Bull AFES 1:29–36
Eglinton G, Hamilton RJ (1967) Leaf epicuticular waxes. Science 156:1322–1335 http://www.jstor.org/stable/1721263
Eglinton G, Logan GA (1991) Molecular preservation. Philos Trans R Soc Lond 333:315–328. https://doi.org/10.1098/rstb.1991.0081
Gocke M, Kuzyakov Y, Wiesenberg GLB (2013) Differentiation of plant derived organic matter in soil, loess and rhizoliths based on n-alkane molecular proxies. Biogeochemistry 112:23–40. https://doi.org/10.1007/s10533-011-9659-y
González-Vila FJ, Lüdemann H-D, Martín F (1983) 13C-NMR structural features of soil humic acids and their methylated, hydrolyzed and extracted derivatives. Geoderma 31:3–15. https://doi.org/10.1016/0016-7061(83)90080-0
IUSS Working Group WRB (2014) World Reference Base for soil resources 2014. International soil classification system for naming soils and creating legends for soil maps. World Soil Resources Reports No. 106. FAO, Rome.
Jambu P, Coulibaly G, Bilong P, Magnoux P, Amblès A (1983) Influence of lipids on the physical properties of soils. In: Novak B (ed) Studies about Humus. Transactions of the VIIth International Symposium. Institute of Crop Protection, Prague, 1:46–50
Jambu P, Moucawi J, Fustec E, Amblès A, Jacquesy R (1985) Inter-relation entre le pH et la nature des composés lipidiques du sol: étude comparée d'une rendzine et d'un sol lessivé glossique. Agrochimica 29:186–198
Jansen B, Wiesenberg GLB (2017) Opportunities and limitations related to the application of plant-derived lipid molecular proxies in soil science. Soil 3:211–234. https://doi.org/10.5194/soil-3-211-2017
Jiménez-González MA, De la Rosa JM, Jiménez-Morillo NT, Almendros G, González-Pérez JA, Knicker H (2016) Post-fire recovery of soil organic matter in a Cambisol from typical Mediterranean forest in Southwestern Spain. Sci Total Environ 572:1414–1421. https://doi.org/10.1016/j.scitotenv.2016.02.134
Jiménez-González MA, Álvarez AM, Carral P, González-Vila FJ, Almendros G (2017) The diversity of methoxyphenols released by pyrolysis-gas chromatography as predictor of soil carbon storage. J Chromatogr A 1508:130–137. https://doi.org/10.1016/j.chroma.2017.05.068
Jiménez-Morillo NT, González-Pérez JA, Jordán A, Zavala LM, De la Rosa JM, Jiménez-González MA, González-Vila FJ (2016) Organic matter fractions controlling soil water repellency in sandy soils from the Doñana National Park (Southwestern Spain). Land Degrad Dev 27:1413–1423. https://doi.org/10.1002/ldr.2314
Jordán A, Zavala LM, Mataix-Solera J, Doerr SH (2013) Soil water repellency: origin, assessment and geomorphological consequences. Catena 108:1–5. https://doi.org/10.1016/j.catena.2013.05.005
Juo ASR, Ayanlaja SA, Ogunwale JA (1976) An evaluation of cation exchange capacity measurements for soils in the tropics. Commun Soil Sci Plant Anal 7:751–761. https://doi.org/10.1080/00103627609366684
Klute A (1986) Water retention: laboratory methods. In: Klute (ed) Methods of soil analysis: part 1—physical and mineralogical methods. American Society of Agronomy and Soil Science Society of America, Madison, pp 635–660
Knicker H (2011) Solid state CPMAS 13C and 15N NMR spectroscopy in organic geochemistry and how spin dynamics can either aggravate or improve spectra interpretation. Org Geochem 42:867–890. https://doi.org/10.1016/j.orggeochem.2011.06.019
Kögel-Knabner I, Hatcher PG (1989) Characterization of alkyl carbon in forest soils by CPMAS 13C NMR spectroscopy and dipolar dephasing. Sci Total Environ 81–82:169–177. https://doi.org/10.1016/0048-9697(89)90122-8
Kögel-Knabner I, Zech W, Hatcher PG (1988) Chemical composition of the organic matter in forest soils: the humus layer. J Plant Nutr Soil Sci 151:331–340. https://doi.org/10.1002/jpln.19881510512
Kononova MM (1966) Soil organic matter: its nature, its role in soil formation and in soil fertility. Pergamon, Oxford
Kruskal JB (1964) Multidimensional scaling by optimizing goodness of fit to a nonmetric hypothesis. Psychometrika 29:1–27. https://doi.org/10.1007/BF02289565
Kumada K, Hurst HM (1967) Green humic acid and its possible origin as a fungal metabolite. Nature 214:631–633. https://doi.org/10.1038/214631a0
Lal R (2004) Soil carbon sequestration to mitigate climate change. Geoderma 123:1–22. https://doi.org/10.1016/j.geoderma.2004.01.032
Legendre P, Legendre L (1998) Numerical ecology, vol 24, 2nd edn. Elsevier Sciences, Amsterdam
Marynowski L, Smolarek J, Hautevelle Y (2015) Perylene degradation during gradual onset of organic matter maturation. Int J Coal Geol 139:17–25. https://doi.org/10.1016/j.coal.2014.04.013
Montiel-Rozas MM, López-García A, Madejón P, Madejón E (2017) Native soil organic matter as a decisive factor to determine the arbuscular mycorrhizal fungal community structure in contaminated soils. Biol Fertil Soils 53:327–338. https://doi.org/10.1007/s00374-017-1181-5
Nath TN (2014) Soil texture and total organic matter content and its influences on soil water holding capacity of some selected tea growing soils in Sivasagar district of Assam, India. Int J Chem Sci 12:1419–1429
Nebbioso A, Vinci G, Drosos M, Spaccini R, Piccolo A (2015) Unveiling the molecular composition of the unextractable soil organic fraction (humin) by humeomics. Biol Fertil Soils 51:443–451. https://doi.org/10.1007/s00374-014-0991-y
Nelson DV, Sommers LE (1982) Total carbon, organic carbon and organic matter. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis: part 2, Chemical and microbiological properties, 2nd edn. American Society of Agronomy, Madison, pp 539–579
Nip M, Tegelaar EW, de Leeuw JW, Schenck PA (1986) A new non-saponifiable highly aliphatic and resistant biopolymer in plant cuticles. Naturwissenschaften 73:579–585
Pendall E, King JY (2007) Soil organic matter dynamics in grassland soils under elevated CO2: insights from long-term incubations and stable isotopes. Soil Biol Biochem 39:2628–2639. https://doi.org/10.1016/j.soilbio.2007.05.016
Prince AL (1945) Determination of total nitrogen, ammonia, nitrates, and nitrites in soils. Soil Sci 59:47–52. https://doi.org/10.1097/00010694-194501000-00007
Requena N, Azcón M, Baca MT (1996) Chemical changes in humic substances from compost due to incubation with ligno-cellulolytic microorganisms and effects on lettuce growth. Appl Microbiol Biotechnol 45:857–863. https://doi.org/10.1007/s002530050774
Rumpel C, Seraphin A, Goebel MO, Wiesenberg G, González-Vila FJ, Bachmann J, Schwark L, Michaelis W, Mariotti A, Kögel-Knabner I (2004) Alkyl C and hydrophobicity in B and C horizons of an acid forest soil. J Plant Nutr Soil Sci 167:685–692. https://doi.org/10.1002/jpln.200421484
Sato O, Kumada K (1967) The chemical nature of the green fraction of P type humic acid. Soil Sci Plant Nutr 13:121–122. https://doi.org/10.1080/00380768.1967.10431985
Schmidt MWI, Torn MS, Abiven S, Dittmar T, Guggenberger G, Janssens IA, Kleber M, Kögel-Knabner I, Lehmann J, Manning DAC, Nannipieri P, Rasse DP, Weiner S, Trumbore SE (2011) Persistence of soil organic matter as an ecosystem property. Nature 478:49–56. https://doi.org/10.1038/nature10386
Simoneit BRT, Mazurek MA (1982) Organic matter of the troposphere—II. Natural background of biogenic lipid matter in aerosols over the rural western United States. Atmos Environ 16:2139–2159. https://doi.org/10.1016/0004-6981(82)90284-0
Stránský K, Streibl M, Herout V (1967) On natural waxes. VI. Distribution of wax hydrocarbons in plants at different evolutionary levels. Collect Czechoslov Chem Commun 32:3213–3220. https://doi.org/10.1135/cccc19673213
Tian J, Lou Y, Gao Y, Fang H, Liu S, Xu M, Blagodatskaya E, Kuzyakov Y (2017) Response of soil organic matter fractions and composition of microbial community to long-term organic and mineral fertilization. Biol Fertil Soils 53:523–532. https://doi.org/10.1007/s00374-017-1189-x
Tinoco P, Almendros G, Sanz J, González-Vila FJ (2002) Comparative analysis of the alkyl breakdown products from soil humic acids by thermal and wet chemical degradation methods. Pyrolysis 2002. 15th International Symposium of Analytical and Applied Pyrolysis. 17–20 September 2002. Leoben, Austria
Walkley A, Black IA (1934) An examination of Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci 37:29–37
Wu G-L, Liu Z-H, Zhang L, Hu T-M, Chen J-M (2010) Effects of artificial grassland establishment on soil nutrients and carbon properties in a black-soil-type degraded grassland. Plant Soil 333:469–479. https://doi.org/10.1007/s11104-010-0363-9
Acknowledgements
The authors are thankful to Prof. Paolo Nannipieri, Editor-in-Chief of Biology and Fertility of Soils and three anonymous referees, who have greatly contributed to improve an early version of this paper.
Funding
The study is financially supported by Spanish CICyT (grant CGL2013-43845-P). Marco A. Jiménez-González is funded by the Spanish Ministry of Economy and Competitiveness (MINECO) for his pre-doctoral FPI fellowship (BES-2014-069238).
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Jiménez-González, M.A., Álvarez, A.M., Hernández, Z. et al. Soil carbon storage predicted from the diversity of pyrolytic alkanes. Biol Fertil Soils 54, 617–629 (2018). https://doi.org/10.1007/s00374-018-1285-6
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DOI: https://doi.org/10.1007/s00374-018-1285-6