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Phosphate solubilization potentials of soil Acinetobacter strains

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Abstract

Many phosphate solubilizing microorganisms (PSM) require external pyrroloquinoline quinone (PQQ) for strong phosphorus (P) solubilization in vitro. The objective of this study was to isolate efficient and PQQ-independent PSM. A total of 21 PSM were isolated from the rhizosphere soil of wheat and maize grown in the pots. Acinetobacter strains were the only PQQ-independent and most effective solubilizers of tricalcium phosphate containing agar. The mean P dissolved in liquid cultures of Acinetobacter strains in a 5-day incubation ranged from 167 to 888 μg/ml P. The pH dropped to below 4.7 from 7.8 in six isolates, which produced gluconic acid in concentrations ranging between 27.5 and 37.5 mM. There was a linear regression between soluble P and gluconic acid concentrations in the bacterial cultures (P < 0.05; R 2 = 0.59). Inoculation with Acinetobacter sp. WR922 significantly (P < 0.05) increased wheat (Triticum aestivum L.) P content by 27% at 15 days after emergence (DAE) and dry matter by 15% at 30 DAE compared to the control. The plant P content in inoculated plants at 30 DAE was linearly correlated with soluble P of the bacterial cultures (P < 0.05; R 2 = 0.69). Gluconic acid production directly affected phosphate solubilization in vitro, which in turn influenced plant P content of inoculated plants in PQQ-independent P-solubilizing Acinetobacter strains.

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Acknowledgment

We gratefully acknowledge the funding of this project by the Gaziosmanpaşa and Selçuk Universities.

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Authors and Affiliations

  1. Cumra Higher Educational College, Selcuk University, Cumra, Konya, Turkey

    Mehmet Ogut & Fatih Er

  2. Agricultural Faculty, Field Crops Department, Gaziosmanpasa University, Tokat, Turkey

    Nejdet Kandemir

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  1. Mehmet Ogut
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  2. Fatih Er
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  3. Nejdet Kandemir
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Correspondence to Mehmet Ogut.

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Ogut, M., Er, F. & Kandemir, N. Phosphate solubilization potentials of soil Acinetobacter strains. Biol Fertil Soils 46, 707–715 (2010). https://doi.org/10.1007/s00374-010-0475-7

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  • DOI: https://doi.org/10.1007/s00374-010-0475-7

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