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Isolation of culturable phosphobacteria with both phytate-mineralization and phosphate-solubilization activity from the rhizosphere of plants grown in a volcanic soil

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Abstract

Chilean volcanic soils contain large amounts of total and organic phosphorus, but P availability is low. Phosphobacteria [phytate-mineralizing bacteria (PMB) and phosphate-solubilizing bacteria (PSB)] were isolated from the rhizosphere of perennial ryegrass (Lolium perenne), white clover (Trifolium repens), wheat (Triticum aestivum), oat (Avena sativa), and yellow lupin (Lupinus luteus) growing in volcanic soil. Six phosphobacteria were selected, based on their capacity to utilize both Na-phytate and Ca-phosphate on agar media (denoted as PMPSB), and characterized. The capacity of selected PMPSB to release inorganic P (Pi) from Na-phytate in broth was also assayed. The results showed that from 300 colonies randomly chosen on Luria–Bertani agar, phosphobacteria represented from 44% to 54% in perennial ryegrass, white clover, oat, and wheat rhizospheres. In contrast, phosphobacteria represented only 17% of colonies chosen from yellow lupin rhizosphere. This study also revealed that pasture plants (perennial ryegrass and white clover) have predominantly PMB in their rhizosphere, whereas PSB dominated in the rhizosphere of crops (oat and wheat). Selected PMPSB were genetically characterized as Pseudomonas, Enterobacter, and Pantoea; all showed the production of phosphoric hydrolases (alkaline phosphatase, acid phosphatase, and naphthol phosphohydrolase). Assays with PMPSB resulted in a higher Pi liberation compared with uninoculated controls and revealed also that the addition of glucose influenced the Pi-liberation capacity of some of the PMPSB assayed.

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Acknowledgements

This study was supported by Bicentenary Program in Science and Technology Grant PSD26, Fondecyt Grant no. 1061262, and International Cooperation Fondecyt Grant no.7060093, CONICYT, Chile. The authors acknowledge the helpful suggestions made by anonymous reviewers to improve the quality of this manuscript.

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Authors and Affiliations

  1. Centro de Ciencias y Biotecnología de Recursos Naturales, Universidad de La Frontera, Casilla 54-D, Temuco, Chile

    Milko A. Jorquera & María de la Luz Mora

  2. Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Casilla 54-D, Temuco, Chile

    Marcela T. Hernández

  3. Soil Science and Plant Nutrition, School of Earth and Geographical Sciences, University of Western Australia, 35 Stirling Highway, Crawley, Western Australia, 6009, Australia

    Zed Rengel

  4. Soil and Land Systems, School of Earth and Environmental Sciences, The University of Adelaide, Adelaide, South Australia, 5005, Australia

    Petra Marschner

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  1. Milko A. Jorquera
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  2. Marcela T. Hernández
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  3. Zed Rengel
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  4. Petra Marschner
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  5. María de la Luz Mora
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Correspondence to María de la Luz Mora.

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Jorquera, M.A., Hernández, M.T., Rengel, Z. et al. Isolation of culturable phosphobacteria with both phytate-mineralization and phosphate-solubilization activity from the rhizosphere of plants grown in a volcanic soil. Biol Fertil Soils 44, 1025–1034 (2008). https://doi.org/10.1007/s00374-008-0288-0

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  • DOI: https://doi.org/10.1007/s00374-008-0288-0

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