Abstract
Wheat cultivars C 306, PBW 175, HD 1553, and HD 2329 were grown in an alkaline soil with and without inorganic P fertilizer and/or farmyard manure in a pot culture experiment. Microbial biomass P (MBP) and alkaline phosphomonoesterase (APM) activities were studied in rhizosphere soils of the above wheat cultivars at different physiological stages. Root weight and P uptake were also estimated simultaneously. Higher microbial biomass P was observed at crown root initiation (CRI) stage while APM activities were higher at panicle initiation (PI) stage. The HD cultivars showed higher MBP and APM activities at PI stage, while at CRI stage, the reverse was true. Though the application of inorganic P apparently showed higher APM activity, the ratio of APM activity and microbial biomass P (APM to MBP) decreased in the presence of inorganic fertilizer P. Inorganic P compared to FYM was the more dominant factor in reducing the APM to MBP ratio. Root weight did not correlate with grain yield. From step-wise regression analysis, it was revealed that microbial biomass P at both CRI and PI stages was a significant factor in influencing the P uptake in relation to grain yield of wheat.
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Beck E, FuBeder A, Kraus M (1989) The maize root system in situ: evaluation of structure and capability of utilization of phytate and inorganic soil phosphates. Z Pflanzenernahr Bodenkd 152:159–167
Bhadraray S, Purakayastha TJ, Chhonkar PK, Verma Vijay (2002) Phosphorus mobilization in hybrid rice rhizosphere compared to high yielding varieties under integrated nutrient management. Biol Fertil Soils 35:73–78
Bhattacharyya P, Datta SC, Dureja P (2003) Interrelationship of pH, organic acids, and phosphorus concentration in soil solution of rhizosphere and non-rhizosphere of wheat and rice crops. Comm Soil Sci Plant Anal 34:231–245
Brookes PC, Powlson DS, Jenkinson DS (1984) Phosphorus in soil microbial biomass. Soil Biol Biochem 16:169–175
Chen GC, He ZL, Huang CY (2000) Microbial biomass phosphorus and its significance in phosphorus availability in red soils. Comm Soil Sci Plant Anal 31:655–667
Clarholm M (1993) Microbial biomass P, labile P, and acid phosphatase activity in the humus layer of a spruce forest, after repeated additions of fertilizers. Biol Fertil Soils 16:287–292
Cosgrove DJ (1967) Metabolism of organic phosphates in soil. In: McClaren AD, Peterson GH (eds) Soil biochemistry, vol I. Marcel Dekker, New York, pp 216–228
Cosgrove DJ (1977) Microbial transformations in the phosphorus cycle. In: Alexander M (ed) Advances in microbial ecology. Plenum, New York, pp 95–135
Curl EA, Truelove B (1986) Root exudates. In: The rhizosphere. Springer, Berlin Heidelberg New York, pp 55–91
Eivazi F, Tabatabai MA (1977) Phosphatases in soils. Soil Biol Biochem 9:167–172
George TS, Gregory PJ, Wood M, Read D, Buresh RJ (2002) Phosphatase activity and organic acids in the rhizosphere of potential agroforestry species and maize. Soil Biol Biochem 34:1487–1494
Greaves MP, Webley DM (1965) A study of the breakdown of organic phosphate by microorganisms from the root region of certain pasture grasses. J Appl Bacteriol 28:423–427
Hayes JE, Richardson AE, Simpson RJ (2000) Components of organic phosphorus in soil extracts that are hydrolyzed by phytase and acid phosphatase. Biol Fertil Soils 32:279–286
Hedley MJ, Stewart WB (1982) Method to measure microbial phosphate in soils. Soil Biol Biochem 14:377–385
Hisinger P (1998) How do plants acquire mineral nutrients? Chemical prices involved in rhizosphere. Adv Agron 64:225–265
Jackson ML (1973) Soil chemical analysis. Prentice Hall, India, New Delhi
Jenkinson DS, Ladd JN (1981) Microbial biomass in soil: measurement and turnover. In: Paul EA, Ladd JN (eds) Soil biochemistry, vol V. Marcel Dekker, New York, pp 415–471
Kirk GJD, Begg CBM, Solivas JL, Barrow NJ (1993) The chemistry of the low land rice rhizosphere. Plant Soil 155–156:83–86
Kirk GJD, Sanlos EE, Sanlos MB (1999) Phosphate solubilization by organic anion secretion form the rice growing in aerobic soil: rates of excretion and decomposition, effects rhizosphere pH and effects on phosphate solubility and uptake. New Phytol 142:185–200
Knudsen D, Peterson GA, Pratt PF (1982) Lithium, sodium and potassium. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, part 2. Chemical and microbiological properties. American Society of Agronomy, Soil Science Society of America, Madison, Wisconsin, pp 225–246
Landi L, Renella G, Moreno JL, Falchini L, Nannipieri P (2000) Influence of cadmium on the metabolic quotient, l-:d-glutamic acid respiration ratio and enzyme activity:microbial biomass ratio under laboratory conditions. Biol Fert Soils 32:8–16
Lindsay WL, Norvell WA (1978) Development of a DTPA test for Zn, Fe and Cu. Soil Sci Soc Am J 42:412–428
Liu ZY, Shi WM, Fan XH (1990) The rhizosphere effects of phosphorus and iron in soils. Transaction of international congress of soil science, Kyoto, Japan II, pp 147–152
Nannipieri P, Johnson RL, Paul EA (1978) Criteria for measurement of microbial growth and activity in soil. Soil Biol Biochem 10:223–229
Neumann G, Romheld V (2001) The release of root exudates as affected by the plant’s physiological status. In: Pinton R, Varanini Z, Nannipieri P (eds) The rhizosphere biochemistry and organic substances at the soil–plant interface. Marcel Dekker, New York, pp 41–93
Olsen SR, Sommers LE (1982) Phosphorus. In: Page AL, Miller RH, Kenney DR (eds) Methods of soil analysis, part 2. Chemical and microbiological properties. American Society of Agronomy, Soil Science Society of America, Madison, Wisconsin, pp 403–430
Otani T, Noriharu A, Tanaka H (1996) Phosphorus uptake mechanism of crops grown in soils with low P status. Soil Sci Plant Nutr 42:553–560
Piper CS (1967) Soil and plant analysis. Asia Publishing House, Bombay, India
Scott Russel R (1982) Plant root systems: their function and interaction with soil. ELBS & McGraw-Hill, UK
Snedecor GW, Cochran WG (1968) Statistical methods. Oxford and IBH, India
Spiers GA, McGill WB (1979) Effects of phosphorus addition and energy supply on acid phosphatase production and activity in soils. Soil Biol Biochem 11:3–8
Subbiah BV, Asija GJ (1956) A rapid method for the estimation of available nitrogen in soils. Curr Sci 25:259–260
Tarafdar JC, Jungk A (1987) Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus. Biol Fertil Soils 3:199–204
Walker TW, Adams AFR (1958) Studies on soil organic matter: I. Influence on phosphorus content of parent materials on accumulation of carbon, nitrogen, sulfur and organic phosphorus in grassland soils. Soil Sci 85:307–318
Walkley AJ, Black IA (1934) Estimation of soil organic carbon by the chromic acid titration method. Soil Sci 37:29–38
Williams CH, Steinbergs H (1959) Soil sulphur fractions as chemical indices of available sulphur in some Australian soils. Aust J Agric Res 10:340–352
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Purakayastha, T.J., Bhadraray, S., Chhonkar, P.K. et al. Microbial biomass phosphorus and alkaline phosphomonoesterase activity in the rhizosphere of different wheat cultivars as influenced by inorganic phosphorus and farmyard manure. Biol Fertil Soils 43, 153–161 (2006). https://doi.org/10.1007/s00374-006-0073-x
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DOI: https://doi.org/10.1007/s00374-006-0073-x