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Calcium response of KCl-excited populations of ventricular myocytes from the European sea bass (Dicentrarchus labrax): a promising approach to integrate cell-to-cell heterogeneity in studying the cellular basis of fish cardiac performance

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Abstract

Climate change challenges the capacity of fishes to thrive in their habitat. However, through phenotypic diversity, they demonstrate remarkable resilience to deteriorating conditions. In fish populations, inter-individual variation in a number of fitness-determining physiological traits, including cardiac performance, is classically observed. Information about the cellular bases of inter-individual variability in cardiac performance is scarce including the possible contribution of excitation–contraction (EC) coupling. This study aimed at providing insight into EC coupling-related Ca2+ response and thermal plasticity in the European sea bass (Dicentrarchus labrax). A cell population approach was used to lay the methodological basis for identifying the cellular determinants of cardiac performance. Fish were acclimated at 12 and 22 °C and changes in intracellular calcium concentration ([Ca2+]i) following KCl stimulation were measured using Fura-2, at 12 or 22 °C-test. The increase in [Ca2+]i resulted primarily from extracellular Ca2+ entry but sarcoplasmic reticulum stores were also shown to be involved. As previously reported in sea bass, a modest effect of adrenaline was observed. Moreover, although the response appeared relatively insensitive to an acute temperature change, a difference in Ca2+ response was observed between 12- and 22 °C-acclimated fish. In particular, a greater increase in [Ca2+]i at a high level of adrenaline was observed in 22 °C-acclimated fish that may be related to an improved efficiency of adrenaline under these conditions. In conclusion, this method allows a rapid screening of cellular characteristics. It represents a promising tool to identify the cellular determinants of inter-individual variability in fishes’ capacity for environmental adaptation.

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Authors and Affiliations

  1. ORPHY, Université de Bretagne Occidentale, Brest, France

    Hélène Ollivier

  2. Unité PFOM-ARN, LEMAR, Centre Ifremer de Brest, Plouzané, France

    James Marchant, Nicolas Le Bayon, Arianna Servili & Guy Claireaux

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  1. Hélène Ollivier
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  2. James Marchant
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  3. Nicolas Le Bayon
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  4. Arianna Servili
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  5. Guy Claireaux
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Correspondence to Hélène Ollivier.

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Communicated by G. Heldmaier.

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Ollivier, H., Marchant, J., Le Bayon, N. et al. Calcium response of KCl-excited populations of ventricular myocytes from the European sea bass (Dicentrarchus labrax): a promising approach to integrate cell-to-cell heterogeneity in studying the cellular basis of fish cardiac performance. J Comp Physiol B 185, 755–765 (2015). https://doi.org/10.1007/s00360-015-0924-6

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