Abstract
In skinned rat cardiac fibres, mitochondrial affinity for endogenous ADP generated by creatine kinase and Ca2+-activated ATPases is higher than for exogenous ADP added to the surrounding medium, suggesting that mitochondria are functionally coupled to creatine kinase and ATPases. Such a coupling may be weaker or absent in ectothermic vertebrate cardiac cells, because they typically have less elaborate intracellular membrane structures, higher glycolytic capacity and lower working temperature. Therefore, we examined skinned cardiac fibres from rainbow trout at 10 °C. The apparent mitochondrial affinity for endogenous ADP was obtained by stimulation with ATP and recording of the release of ADP into the surrounding medium. The apparent affinity for endogenous ADP was much higher than for exogenous ADP suggesting a functional coupling between mitochondria and ATPases. The apparent affinity for exogenous ADP and ATP was increased by creatine or an increase in Ca2+-activity, which should increase intrafibrillar turnover of ATP to ADP. In conclusion, ADP seems to be channelled from creatine kinase and ATPases to mitochondria without being released to the surrounding medium. Thus, despite difference in structure, temperature and metabolic capacity, trout myocardium resembles that of rat with regard to the regulation of mitochondrial respiration.
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Abbreviations
- ACR :
-
acceptor control ratio
- ANT :
-
adenine nucleotide translocase
- K M ADP :
-
apparent mitochondrial affinity for ADP
- K M ATP :
-
apparent mitochondrial affinity for ATP
- LDH :
-
lactate dehydrogenase
- V ADP :
-
ADP-stimulated respiration rate
- V ADP max :
-
maximal ADP-stimulated respiration rate
- V ATP :
-
ATP-stimulated respiration rate
- V ATP max :
-
maximal ATP-stimulated respiration rate
- V 0 :
-
basal respiration rate in the absence of ADP
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We wish to acknowledge the support of this study by the Danish Research Council and engineer Einer Larsen for the construction of the computer program used for collection of data.
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Communicated by G. Heldmaier
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Birkedal, R., Gesser, H. Regulation of mitochondrial energy production in cardiac cells of rainbow trout (Oncorhynchus mykiss). J Comp Physiol B 174, 255–262 (2004). https://doi.org/10.1007/s00360-003-0410-4
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DOI: https://doi.org/10.1007/s00360-003-0410-4