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The impact of klotho gene polymorphisms on urinary tract stone disease

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Abstract

Objectives

To investigate the effect of klotho gene and β-glucuronidase activity on stone formation in patients with urinary tract stone disease (UTSD).

Methods

A total of 103 patients with UTSD and 102 controls with no specific urolithiasis history were enrolled into the study. G395A and C1818T polymorphisms of klotho gene were analyzed with PCR method. Serum levels of calcium and phosphorus and 24-h urine levels of β-glucuronidase activity, calcium and phosphorus levels were measured biochemically.

Results

A total of 103 of patients were male (50.2 %) and 102 were female (49.8 %) (p 0.945). Twenty-four-hour urine levels of calcium were significantly higher in UTSD group, whereas no difference was observed in phosphorus levels (p < 0.001, p 0.074, respectively). As for the G395A polymorphism, type of GG was significantly higher in the patient group compared to the controls (p = 0.02), while GA genotype was significantly higher in the controls (p = 0.001). There was no significant difference in F352V and C1818T polymorphism between the patient and control groups. β-glucuronidase activity was slightly lower in the patient group without significance (p 0.932).When patients with GG genotype and the rest were compared, there were no significant difference in all parameters.

Conclusions

Any polymorphism altering the function of klotho gene may result with stone formation. We found that there are more GG sequences of G395A gene in patients with UTSD. That may be a polymorphism of klotho gene which results with stone formation. Further studies with more patients should be accomplished which are combining the genetic and epigenetic factors associated with urolithiasis and klotho gene to enlighten the etiology of this disease.

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Author’s contribution

Gürel contributed to data collection or management, data analysis and manuscript writing; Üre contributed to data analysis and manuscript writing; Temel and Aslan developed the protocol/project; Çilingir developed the protocol/project and analyzed data; Uslu and Celayir contributed to data collection or management; and Başeskioğlu developed the protocol/project and wrote the manuscript.

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Authors and Affiliations

  1. Department of Urology, Faculty of Medicine, Osmangazi University, Eskisehir, Turkey

    Abdullah Gürel, İyimser Üre & Ali Barbaros Başeskioğlu

  2. Department of Medical Biochemistry, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey

    Halide Edip Temel

  3. Department of Medical Genetics, Faculty of Medicine, Osmangazi University, Eskisehir, Turkey

    Oğuz Çilingir & Serap Aslan

  4. Department of Medical Biochemistry, Faculty of Medicine, Osmangazi University, Eskisehir, Turkey

    Sema Uslu

  5. Department of Urology, Şevket Yılmaz Education and Research Hospital, Bursa, Turkey

    Mehmet Fatih Celayir

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  1. Abdullah Gürel
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  2. İyimser Üre
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  3. Halide Edip Temel
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  7. Serap Aslan
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  8. Ali Barbaros Başeskioğlu
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Correspondence to İyimser Üre.

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Gürel, A., Üre, İ., Temel, H.E. et al. The impact of klotho gene polymorphisms on urinary tract stone disease. World J Urol 34, 1045–1050 (2016). https://doi.org/10.1007/s00345-015-1732-z

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  • DOI: https://doi.org/10.1007/s00345-015-1732-z

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