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Comparative Genome-Wide Analysis and Expression Profiling of Histone Acetyltransferases and Histone Deacetylases Involved in the Response to Drought in Wheat

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Abstract

Histone acetyltransferases (HATs) and histone deacetylases (HDACs) contribute to plant growth, development, and stress responses. A number of HAT and HDAC genes have been identified in several plants. However, wheat HATs and HDACs have not been comprehensively characterized. In this study, 30 TaHAT genes and 53 TaHDAC genes were detected in the wheat genome. As described in other plants, TaHATs were classified into four subfamilies (i.e., GNAT, p300/CBP, MYST, and TAFII250) and TaHDACs were divided into three subfamilies (i.e., RPD3/HDA1, HD2, and SIR2). Phylogenetic and conserved domain analyses showed that TaHATs and TaHDACs are highly similar to those in Arabidopsis and rice; however, divergence and expansion from Arabidopsis and rice were also observed. We detected many stress-related cis-regulatory elements in the promoter regions of these genes (i.e., ABRE, STRE, MYB, etc.). Further, based on a comparative expression analyses of three varieties with different degrees of drought resistance under drought stress, we found that TaHAG2, TaHAG3, TaHAC2, TaHDA18, TaHDT1, and TaHDT2 are likely regulate drought stress in wheat.

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Abbreviations

HATs:

Histone acetyltransferases

HDACs:

Histone deacetylases

GNAT:

GCN5-related N-terminal acetyltransferase

p300/CBP:

CREB-binding protein

MYST:

MOZ, Ybf2/Sas3, Sas2, and Tip60

TAFII250:

TATA-binding protein-associated factors

RPD3/HDA1:

Reduced Potassium Dependency 3/Histone DeAcetylase 1

SIR2:

Silent Information Regulator 2

HD2:

Histone Deacetylase 2

pI:

The theoretical isoelectronic point

ABRE:

Abscisic acid-responsive element

STRE:

Stress-responsive element

ARE:

Essential for anaerobic induction

CCGTCC-box:

Meristem-specific activation

G-Box:

Light responsiveness

MYB:

MYB-related binding sites

TGA-element:

Auxin-responsive element

PSII:

Photosystem II

LHCb:

Light-harvesting chlorophyll a/b-binding proteins

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Acknowledgments

This research was supported by the National Key Research and Development Program of China (Grant No. 2017YFD0300408) and Natural Science Foundation of Henan Province (Grant No. 212300410352).

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Author notes

  1. Hua Li and Huajie Liu have contributed equally to this study.

Authors and Affiliations

  1. College of Life Science, Henan Agricultural University, Zhengzhou, China

    Hua Li, Huajie Liu, Xinxin Pei, Hongyu Chen & Xiao Li

  2. College of Agronomy/State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, China

    Jiarui Wang & Chenyang Wang

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  1. Hua Li
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Contributions

HL designed the experiments and wrote the manuscript. HL and HJL carried out the bioinformatics analysis. XXP and HYC performed the biological experiment. XL and JRW prepared the plant sample. CYW helped revise the manuscript. All the authors read and approved the final manuscript.

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Correspondence to Hua Li or Chenyang Wang.

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Li, H., Liu, H., Pei, X. et al. Comparative Genome-Wide Analysis and Expression Profiling of Histone Acetyltransferases and Histone Deacetylases Involved in the Response to Drought in Wheat. J Plant Growth Regul 41, 1065–1078 (2022). https://doi.org/10.1007/s00344-021-10364-9

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