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Application of Melatonin-Enhanced Tolerance to High-Temperature Stress in Cherry Radish (Raphanus sativus L. var. radculus pers)

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Abstract

The growth and development of cold-season plants are susceptible to high temperature. Melatonin is a plant growth regulator with potential to improve plant tolerance to biological and abiotic stresses. In our study, cherry radish (Raphanus sativus L. var. radculus pers) was cultured at a high temperature (35 °C/30 °C day/night) and different concentrations (0, 11.6, 17.4, 29.0, 34.8 and 67.0 mg L−1) of melatonin were applied to these high-temperature stressed plants to its effects on biomass, quality, antioxidant enzyme activity, chlorophyll and endogenous hormone contents. The plants were grown under normal temperature (25 °C/20 °C) as control and high-temperature condition as HT-stress treatment. The results revealed that under high temperature with 29.0 mg L−1 melatonin treatment, cherry radish biomass was significantly increased by 12.9%, and the soluble protein and soluble solid were increased by 18.7 and 9.2%, respectively. The activity of antioxidant enzyme, ascorbate peroxidase and peroxidase were increased by 43.7 and 45.5%, respectively. The chlorophyll a and carotenoid contents were increased by 7.4 and 20.0% compared with the control at 27 days. The auxin and abscisic acid contents were significantly increased by 28.5 and 6.7% compared with HT at 9 days. Thus, application of optimal rate of melatonin had a positive effect on cherry radish growth under high-temperature stress.

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Acknowledgements

The study was supported by the National Key Research and Development Program of China (Grant No. 2017YFD0200706) and the National Natural Science Foundation of China (Grant No. 41571236). Shandong educational reform fund for undergraduate (Z2016M022).

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Authors and Affiliations

  1. National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, National Engineering & Technology Research Center for Slow and Controlled Release Fertilizers, College of Resources and Environment, Shandong Agricultural University, Tai’an, 271018, Shandong, China

    Chunhua Jia, Xiaojing Yu, Min Zhang, Zhiguang Liu & Yachun Xu

  2. State Key Laboratory of Nutrition Resources Integrated Utilization, Kingenta Ecological Engineering Group Co., Ltd, Linshu, 276700, Shandong, China

    Min Zhang & Peng Zou

  3. Hulunbeier NewGold Chemical Co., Ltd, Hailaer, Hulunbeier, 021000, Inner Mongolia, China

    Jun Ma

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  1. Chunhua Jia
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  4. Zhiguang Liu
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Jia, C., Yu, X., Zhang, M. et al. Application of Melatonin-Enhanced Tolerance to High-Temperature Stress in Cherry Radish (Raphanus sativus L. var. radculus pers). J Plant Growth Regul 39, 631–640 (2020). https://doi.org/10.1007/s00344-019-10006-1

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