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The Possible Roles of Priming with ZnO Nanoparticles in Mitigation of Salinity Stress in Lupine (Lupinus termis) Plants

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Abstract

To our knowledge, little attention has been paid to evaluating ZnO nanoparticles (ZNPs) roles in plants grown under salinity stress. In this study, seeds of lupine (Lupinus termis) plants were grown in plastic pots and exposed to 0 (control) and 150 (S) mM NaCl with or without priming with different concentrations of ZnO [20 mg L−1 (ZNPs1), 40 mg L−1 (ZNPs2), and 60 mg L−1 (ZNPs3)] for 20 days. Salinized plants showed a reduction in plant growth parameters (root length, shoot length, fresh weight, and dry weight) and in the contents of photosynthetic pigments (chlorophyll a and b, and carotenoids) and Zn, as well as in the activity of catalase (CAT) against control plants. On the other side, salinity stress boosted the contents of organic solutes (soluble sugar, soluble protein, total free amino acids, and proline), total phenols, malondialdehyde (MDA), ascorbic acid and Na, as well as the activities of superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX) in stressed plants over control plants. However, seed-priming with ZNPs mostly stimulated growth of stressed plants, which was accompanied by reinforcement in the levels of photosynthetic pigments, organic solutes, total phenols, ascorbic acid and Zn, as well as in the activities of SOD, CAT, POD, and APX enzymes over stressed plants alone. On the contrary, priming with ZNPs caused a decrement in the contents of MDA and Na in stressed plants relative to salinized plants alone. It is worthy to mention that, this improvement in salt tolerance of plants primed with ZNPs was more obvious in plants primed with ZNPs3 and grown both in unstressed and stressed regimes. Thus, our findings suggest that seed-priming with ZNPs, especially 60 mg L−1 ZnO is an effective strategy that can be used to enhance salt tolerance of lupine plants.

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References

  • Abdel Latef AA (2010) Changes of antioxidative enzymes in salinity tolerance among different wheat cultivars. Cereal Res Commun 38:43–55

    Article  Google Scholar 

  • Abdel Latef AA (2011) Ameliorative effect of calcium chloride on growth, antioxidant enzymes, protein patterns and some metabolic activities of canola (Brassica napus L.) under seawater stress. J Plant Nutr 34:1303–1320

    Article  CAS  Google Scholar 

  • Abdel Latef AA, Chaoxing H (2011) Effect of arbuscular mycorrhizal fungi on growth, mineral nutrition, antioxidant enzymes activity and fruit yield of tomato grown under salinity stress. Sci Hortic 127:228–233

    Article  CAS  Google Scholar 

  • Abdel Latef AA, Chaoxing H (2014) Does the inoculation with Glomus mosseae improve salt tolerance in pepper plants? J Plant Growth Regul 33:644–653

    Article  CAS  Google Scholar 

  • Abdel Latef AA, Tran LSP (2016) Impacts of priming with silicon on the growth and tolerance of maize plants to alkaline stress. Front Plant Sci 7:243

    Article  PubMed  PubMed Central  Google Scholar 

  • Aebi H (1984) Catalase in vitro. Method Enzym 105:121–126

    Article  CAS  Google Scholar 

  • Ahmad P, Latef AAA, Hashem A, Abd_Allah EF, Gucel S, Tran LSP (2016) Nitric oxide mitigates salt stress by regulating levels of osmolytes and antioxidant enzymes in chickpea. Front Plant Sci 7:347

    PubMed  PubMed Central  Google Scholar 

  • Ali EA, Mahmoud AM (2013) Effect of foliar spray by different salicylic acid and zinc concentration on seed yield components of mungbean in sandy soil. Asian J Crop Sci 5:33–40

    Article  Google Scholar 

  • Allen SE (1989) Chemical analysis of ecological materials, 2nd edn. Blackwell, Oxford

    Google Scholar 

  • Auld DS (2001) Zinc coordination sphere in biochemical zinc sites. Biometals 14:271–313

    Article  CAS  PubMed  Google Scholar 

  • Azooz MM, Shaddad MA, Abdel-Latef AA (2004) The accumulation and compartmentation of proline in relation to salt tolerance of three sorghum cultivars. Ind J Plant Physiol 9:1–8

    CAS  Google Scholar 

  • Bates LS, Wladren PR, Tear DT (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Brayner R, Ferrari-Iliou R, Brivois N, Djediat S, Benedetti MF, Fievet F (2006) Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium. Nano Lett 6:866–870

    Article  CAS  PubMed  Google Scholar 

  • Burman U, Saini M, Kumar Praveen (2013) Effect of zinc oxide nanoparticles on growth and antioxidant system of chickpea seedlings. Toxicol Environ Chem 95:605–612

    Article  CAS  Google Scholar 

  • Cakmak I (2000) Possible roles of zinc in protecting plant cells from damage by reactive oxygen species. New Phytol 146:185–205

    Article  CAS  Google Scholar 

  • Chen G, Asada K (1992) Inactivation of ascorbate peroxidase by thoils requires hydrogen peroxide. Plant Cell Physiol 33:117–123

    CAS  Google Scholar 

  • Choudhury SR, Ghosh M, Mandal A, Chakravorty D, Pal M, Pradhan S, Goswami A (2011) Surface-modified sulfur nanoparticles: an effective antifungal agent against Aspergillus niger and Fusarium oxysporum. Appl Microbiol Biotechnol 90:733–743

    Article  PubMed  Google Scholar 

  • Choudhury SR, Roy S, Goswami A, Basu S (2012) Polyethylene glycol-stabilized sulphur nanoparticles: an effective antimicrobial agent against multidrug-resistant bacteria. J Antimicrob Chemother 67:1134–1137

    Article  PubMed  Google Scholar 

  • de la Rosa G, Lopez-Moreno ML, de Haro D, Botez CE, Peralta-Videa JR, Gardea-Torresdey JL (2013) Effects of ZnO nanoparticles in alfalfa, tomato, and cucumber at the germination stage: root development and X-ray absorption spectroscopy studies. Pure Appl Chem 85:2161–2174

    Google Scholar 

  • Ebrahimian E, Bybordi A (2011) Exogenous silicium and zinc increase antioxidant enzyme activity and alleviate salt stress in leaves of sunflower. J Food Agri Env 9:422–427

    CAS  Google Scholar 

  • Fan Z, Lu JG (2005) Zinc oxide nanostructures: synthesis and properties. J Nanosci Nanotechnol 5:1561–1573

    Article  CAS  PubMed  Google Scholar 

  • Gerber C, Lang HP (2006) How the doors to the nanoworld were opened. Nat Nanotechnol 1:3–5

    Article  CAS  PubMed  Google Scholar 

  • Giannopolitis CN, Ries SK (1977) Superoxide dismutases. I. Occurrence in higher plants. Plant Physiol 59:309–314

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Review. Plant Physiol Biochem 48:909–930

    Article  CAS  PubMed  Google Scholar 

  • Hajiboland R (2013) Reactive oxygen species and photosynthesis. In: Ahmad P (ed) Oxidative damage to plants. Elsevier Inc, New York, pp 1–63

    Google Scholar 

  • Ibrahim S, Faryal S (2014) Augmentation of Trigonella foenum-graecum L. (methi) growth under salinity stress and allelochemical stress through Mn + B + Zn mixture foliar spray. J Pharmac Phytochem 3:39–44

    Google Scholar 

  • Irigoyen JJ, Emerich DW, Sanchez-Dıaz M (1992) Water stress induced changes in concentrations of proline and total soluble sugar in nodulated alfalfa (Medicago sativa) plants. Physiol Plant 84:55–60

    Article  CAS  Google Scholar 

  • Jiang W, Sun XH, Xu HL, Mantri N, Lu HF (2014) Optimal concentration of zinc sulfate in foliar spray to alleviate salinity stress in Glycine soja. J Agr Sci Tech 16:445–460

    CAS  Google Scholar 

  • Jones N, Ray B, Ranjit KT, Manna AC (2008) Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiol Lett 279:71–76

    Article  CAS  PubMed  Google Scholar 

  • Kah M (2015) Nanopesticides and nanofertilizers: emerging contaminants or opportunities for risk mitigation? Front Chem 3:64

    Article  PubMed  PubMed Central  Google Scholar 

  • Katsuhara M, Otsuka T, Ezaki B (2005) Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis. Plant Sci 169:369

    Article  CAS  Google Scholar 

  • Khodary SEA (2004) Effect of NaCl salinity on improvement of nitrogen metabolism and some ions uptake in lupine plants subjected to gamma irradiation. Int J Agric Biol 1:1–4

    Google Scholar 

  • Klapheck S, Zimmer I, Cosse H (1990) Scavenging of hydrogen peroxide in the endosperm of Ricinus communis by ascorbate peroxidase. Plant Cell Physiol 31:1005–1013

    CAS  Google Scholar 

  • Laware SL, Raskar SV (2014) Effect of zinc oxide nanoparticles on cytology and seed germination in onion. Int J Curr Microbiol App Sci 3:467–473

    Google Scholar 

  • Lee YP, Takanashi T (1966) An improved colorimetric determination of amino acids with the use of ninhydrin. Anal Biochem 14:71–77

    Article  CAS  Google Scholar 

  • Li WYF, Wong FL, Tsai SN, Tsai SN, Phang TH, Shao GH, Lam HM (2006) Tonoplast-located GmCLC1 and GmNHX1 from soybean enhance NaCl tolerance in transgenic bright yellow (by)-2 cells. Plant Cell Environ 29:1122–1137

    Article  CAS  PubMed  Google Scholar 

  • Lichtenthaler HK, Wellburn RR (1983) Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11:591–592

    Article  CAS  Google Scholar 

  • Lin CC, Kao CH (2000) Effect of NaCl stress on H2O2 metabolism in rice leaves. Plant Growth Regul 30:151–155

    Article  CAS  Google Scholar 

  • Maehly AC, Chance B (1954) The assay of catalase and peroxidase. In: Glick D (ed) Methods in biochemistry analysis, vol 1. Interscience Publishers, New York, pp 357–425

    Chapter  Google Scholar 

  • Mahajan P, Dhoke SK, Khanna AS (2011) Effect of nano-ZnO particle suspension on growth of mung (Vigna radiata) and gram (Cicer arietinum) seedlings using plant agar method. J Nanotechnol 2011:1–7

    Article  Google Scholar 

  • Mohamed AA, Eichler-Löbermann B, Schnug E (2007) Response of crops to salinity under Egyptian conditions: a review. Landbauforsch Völkenrode 2:119–125

    Google Scholar 

  • Mukherjee SP, Choudhari MA (1983) Implications of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58:116–170

    Article  Google Scholar 

  • Mukherjee A, Peralta-Videa JR, Bandyopadhyay S, Rico CM, Zhaob L, Gardea-Torresdey JL (2014) Physiological effects of nanoparticulate ZnO in green peas (Pisum sativum L.) cultivated in soil. Metallomics 6:132–138

    Article  CAS  PubMed  Google Scholar 

  • Nel A, Xia T, Madler L, Li N (2006) Toxic potential of materials at the nano level. Science 311:622–627

    Article  CAS  PubMed  Google Scholar 

  • Pathak GC, Gupta B, Pandey N (2012) Improving reproductive efficiency of chickpea by foliar application of zinc Braz. J Plant Physiol 24:173–180

    CAS  Google Scholar 

  • Patra P, Choudhury SR, Mandal S, Basu A, Goswami A, Gogoi R, Srivastava C, Kumar R, Gopal M (2013) Effect sulfur and ZnO nanoparticles on stress physiology and plant (Vigna radiata) nutrition. In: Giri PK (ed) Advanced nanomaterials and nanotechnology. Springer, Berlin, pp 301–309

    Chapter  Google Scholar 

  • Prasad TNVKV, Sudhakar P, Sreenivasulu Y, Latha P, Munaswamy V, Reddy KR, Sreeprasad TS, Sajanlal PR, Pradeep T (2012) Effect of nanoscale zinc oxide particles on the germination, growth and yield of peanut. J Plant Nutr 35:905–927

    Article  CAS  Google Scholar 

  • Raliya R, Tarafdar JC (2013) ZnO nanoparticle biosynthesis and its effect on phosphorous-mobilizing enzyme secretion and gum contents in cluster bean (Cyamopsis tetragonoloba L.). Agric Res 2:48–57

    Article  CAS  Google Scholar 

  • Ramesh M, Palanisamy K, Babu K, Sharma NK (2014) Effects of bulk & nano-titanium dioxide and zinc oxide on physio-morphological changes in Triticum aestivum Linn. J Glob Biosci 3:415–422

    Google Scholar 

  • Ranjit SL, Manish P, Penna S (2016) Early osmotic, antioxidant, ionic, and redox responses to salinity in leaves and roots of Indian mustard (Brassica juncea L.). Protoplasma 253:101–110

    Article  CAS  PubMed  Google Scholar 

  • Rezaei M, Abbasi H (2014) Foliar application of nanochelate and non-nanochelate of zinc on plant resistance physiological processes in cotton (Gossipium hirsutum L.). Iran J Plant Physiol 4:1137–1144

    Google Scholar 

  • Rockenfeller P, Madeo F (2008) Apoptotic death of ageing yeast. Exp Gerontol 43:876–881

    Article  CAS  PubMed  Google Scholar 

  • Sedghi M, Hadi M, Toluie SG (2013) Effect of nano zinc oxide on the germination of soybean seeds under drought stress. Ann West Uni Timisoaraser Biol 2:73–78

    Google Scholar 

  • Siddiqui MH, Al-Whaibi MH, Firoz M, Al-Khaishany MY (2015) Role of nanoparticles in plants. In: Siddiqui MH (ed) Nanotechnology and plant sciences. Springer, Basel, pp 19–35

    Google Scholar 

  • Singh AK, Dubey RS (1995) Changes in chlorophyll a and b contents and activities of photosystems 1 and 2 in rice seedlings induced by NaCl. Photosythetica 31:489–499

    CAS  Google Scholar 

  • Singh P, Nanda A (2014) Enhanced sun protection of nano-sized metal oxide particles over conventional metal oxide particles: an in vitro comparative study. Int J Cosmet Sci 36:273–283

    Article  CAS  PubMed  Google Scholar 

  • Skerget M, Kotnik P, Hadolin M, Hras A, Simonic M, Knez Z (2005) Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem 89:191–198

    Article  CAS  Google Scholar 

  • Soliman AS, El-feky SA, Darwish E (2015) Alleviation of salt stress on Moringa peregrina using foliar application of nanofertilizers. J Hortic Fores 7:36–47

    Article  CAS  Google Scholar 

  • Weisany W, Sohrabi Y, Heidari G, Siosemardeh A, Ghassemi-Golezani K (2011) Physiological responses of soybean (Glycine max L.) to zinc application under salinity stress. Aust J Crop Sci 5:1441–1447

    CAS  Google Scholar 

  • Weisany W, Sohrabi Y, Heidari G, Siosemardeh A, Ghassemi-Golezani K (2012) Changes in antioxidant enzymes activity and plant performance by salinity stress and zinc application in soybean (Glycine max L.). Plant Omics J 5:60–67

    CAS  Google Scholar 

  • Yasmeen A, Basra SMA, Farooq M, Rehman H, Hussain N, Athar HR (2013) Exogenous application of moringa leaf extract modulates the antioxidant enzyme system to improve wheat performance under saline conditions. Plant Growth Regul 69:225–233

    Article  CAS  Google Scholar 

  • Zheng JL, Zhao LY, Shen B, Jiang LH, Zhu AY (2016) Effects of salinity on activity and expression of enzymes involved in ionic, osmotic, and antioxidant responses in Eurya emarginata. Acta Physiol Plant 38:1–9

    Article  Google Scholar 

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Author contributions

Arafat Abdel Hamed Abdel Latef conceived and designed the experiments. Khaled Ebnalwaled Abdelfattah prepared ZnO nanoparticles. Arafat Abdel Hamed Abdel Latef and Mona Fawzy Abu Alhmad conducted the experiments. Mona Fawzy Abu Alhmad collected the data. Arafat Abdel Hamed Abdel Latef analyzed the data and wrote the manuscript.

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Authors and Affiliations

  1. Botany Department, Faculty of Science, South Valley University, Qena, 83523, Egypt

    Arafat Abdel Hamed Abdel Latef & Mona Fawzy Abu Alhmad

  2. Biology Department, College of Applied Medical Science, Turabah Branch, Taif University, Taif, 21955, Saudi Arabia

    Arafat Abdel Hamed Abdel Latef

  3. Electronics & Nano Devices Lab., Physics Department, Faculty of Science, South Valley University, Qena, 83523, Egypt

    Khaled Ebnalwaled Abdelfattah

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  1. Arafat Abdel Hamed Abdel Latef
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  2. Mona Fawzy Abu Alhmad
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  3. Khaled Ebnalwaled Abdelfattah
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Correspondence to Arafat Abdel Hamed Abdel Latef.

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Abdel Latef, A.A.H., Abu Alhmad, M.F. & Abdelfattah, K.E. The Possible Roles of Priming with ZnO Nanoparticles in Mitigation of Salinity Stress in Lupine (Lupinus termis) Plants. J Plant Growth Regul 36, 60–70 (2017). https://doi.org/10.1007/s00344-016-9618-x

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