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Molecular detection of microbial communities associated with Microcystis vs Synechococcus dominated waters in Tianjin, China

  • ICTC-10 Special Issue: Cyanobacteria and cyanotoxins: responses and detection
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Abstract

Harmful cyanobacterial blooms cause many ecological disasters worldwide. During the development of cyanobacterial blooms, the diversity and domination of cyanobacterial taxa are of a particular concern. In this study, the microbial community structure within a water system, such as in Yuqiao Reservoir and Haihe River in Tianjin City, China, was compared by using next-generation sequencing. A total of 5 001 operational taxonomic units were obtained and clustered from filtered 16S rDNA V3–V4 region sequences. The cyanobacterial and microbial structures greatly differed in these two water areas. Microcystis was dominant in Yuqiao, whereas Synechococcus was dominant in Haihe. Proteobacteria species were dominant among all detected samples. The relative abundances of Bacteroidetes and Planctomycetes were higher in Yuqiao Reservoir than in Haihe River, whereas Firmicutes and Verrucomicrobia were relatively abundant in Haihe River. Further analyses indicated that the domination of both cyanobacteria was strongly related to several environmental factors, such as total nitrogen, total phosphorus, and dissolved oxygen, reflecting the role of trophic states in shaping the dominance of cyanobacterial taxa. The present study provided the example for Microcystis and Synechococcus dominance along a cyanobacterial bloom in north China. Applying highthroughput sequencing could offer a wide field of vision in analyzing microbial community structures.

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Acknowledgment

We would like to thank ZHENG Tao, JI Dongdong, QIN Yiming and LI Na at Tianjin Agricultural University for their work to sample collecting. Especially thank to Ms. CAO Qi for her support to data arrangement.

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Authors and Affiliations

  1. Department of Fisheries Sciences, Tianjin Agricultural University, National Demonstration Center for Experimental Aquaecology and Aquaculture Education, Tianjin, 300384, China

    Da Huo  (霍达), Yiming Li  (李一鸣) & Zhiyi Qiao  (乔之怡)

  2. Key Laboratory of Algal Biology, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China

    Da Huo  (霍达), Youxin Chen  (陈友信) & Renhui Li  (李仁辉)

  3. Department of Basic Sciences, Tianjin Agricultural University, Tianjin, 300384, China

    Ping Liu  (刘萍)

  4. University of Chinese Academy of Sciences, Beijing, 100049, China

    Youxin Chen  (陈友信)

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  1. Da Huo  (霍达)
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  2. Youxin Chen  (陈友信)
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  3. Ping Liu  (刘萍)
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  6. Renhui Li  (李仁辉)
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Corresponding authors

Correspondence to Zhiyi Qiao  (乔之怡) or Renhui Li  (李仁辉).

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Supported by the International Science & Technology Cooperation Program of China (No. 2013DFA71340) and the National Natural Science Foundation of China (No. 51779247)

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Huo, D., Chen, Y., Liu, P. et al. Molecular detection of microbial communities associated with Microcystis vs Synechococcus dominated waters in Tianjin, China. J. Ocean. Limnol. 36, 1145–1156 (2018). https://doi.org/10.1007/s00343-018-7182-x

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