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Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis

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Abstract

Thioredoxin (Trx) proteins are involved in many biological processes especially the regulation of cellular redox homeostasis. In this study, two Trx cDNAs were cloned from the mussel Mytilus galloprovincialis using rapid amplifi cation of cDNA ends-polymerase chain reaction (RACE-PCR). The two cDNAs were named MgTrx1 and MgTrx2, respectively. The open reading frames of MgTrx1 and MgTrx2 were 318 and 507 base pairs (bp) and they encoded proteins of 105 and 168 amino acids with estimated molecular masses of 11.45 and 18.93 kDa, respectively. Sequence analysis revealed that both proteins possessed the conserved active site dithiol motif Cys-Gly-Pro-Cys. In addition, MgTrx2 also possessed a putative mitochondrial targeting signal suggesting that it is located in the mitochondria. Quantitative real-time polymerase chain reaction (qPCR) revealed that both MgTrx1 and MgTrx2 were constitutively expressed in all tissues examined. The MgTrx1 transcript was most abundant in hemocytes and gills, whereas the MgTrx2 transcript was most abundant in gonad, hepatopancreas, gill and hemocytes. Following Vibrio anguillarum challenge, the expression of MgTrx1 was up-regulated and reached its peak, at a value 10-fold the initial value, at 24 h. Subsequently, expression returned back to the original level. In contrast, the expression level of MgTrx2 was down-regulated following bacterial stimulation, with one fi fth of the control level evident at 12 h post challenge. These results suggest that MgTrx1 and MgTrx2 may play important roles in the response of M. galloprovincialis to bacterial challenge.

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Authors and Affiliations

  1. Key Laboratory of Coastal Zone Environmental Processes, Chinese Academy of Science; Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China

    Qing Wang  (王清), Jianmin Zhao  (赵建民), Liping You  (由丽萍), Chunyan Wang  (王春艳) & Huifeng Wu  (吴惠丰)

  2. Yantai Oceanic Environmental Monitoring Central Station of SOA, Yantai, 264006, China

    Xuanxuan Ning  (宁璇璇)

  3. China Agriculture University (Yantai), Yantai, 264670, China

    Dong Pei  (裴东)

Authors
  1. Qing Wang  (王清)
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  2. Xuanxuan Ning  (宁璇璇)
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  3. Dong Pei  (裴东)
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  4. Jianmin Zhao  (赵建民)
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  5. Liping You  (由丽萍)
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  6. Chunyan Wang  (王春艳)
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  7. Huifeng Wu  (吴惠丰)
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Corresponding authors

Correspondence to Jianmin Zhao  (赵建民) or Huifeng Wu  (吴惠丰).

Additional information

Supported by the National Natural Science Foundation of China (No. 31172388), the 100 Talents Program of the Chinese Academy of Sciences, and the Key Laboratory for Ecological Environment in Coastal Areas (201011), State Oceanic Administration

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Wang, Q., Ning, X., Pei, D. et al. Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis . Chin. J. Ocean. Limnol. 31, 493–503 (2013). https://doi.org/10.1007/s00343-013-2234-8

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  • DOI: https://doi.org/10.1007/s00343-013-2234-8

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