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Quantitative phase detection with expanded principal component analysis method on interferometric microscopic cytometer

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Abstract

Based on interferometric microscopy, we develop a quantitative interferometric microscopic cytometer with expanded principal component analysis (PCA) phase retrieval method to obtain phase distributions of numerous biological samples with spatial resolution ~1.5 μm. The expanded PCA method realizes high-speed phase imaging from multiple microscopic interferograms captured by CCD camera when the biological samples are scanned in the field of view. This method is a time-domain algorithm which calculates faster than traditional frequency-domain algorithms and overcomes drawbacks induced by fast Fourier transform. The potential of this phase detecting system for studying biological systems is demonstrated with simulations and phase measurement of red blood cells in experiments.

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Acknowledgments

This work is supported by the National Natural Science Foundation of China under grants 10704039 and 10904072. Authors thank Institute of Veterinary Medicine of Jiangsu Province Academy of Agricultural Sciences for kindly providing rabbit red blood cells. We thank Prof. Fei Liu in Nanjing Agricultural University (NAU) for sample preparation and suggestions. We also thank Dr. Haijiao Jiang (now in Nanjing Institute of Astronomical Optics and Technology, CAS), Dr. Nan Sun (now in Shanghai Institute of Quality Inspection and Technical Research), Yanke Shan (NAU), Keding Yan (NUST) and Xian Tang (NUST) for their kindly help and discussion in the experiments.

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Authors and Affiliations

  1. Department of Information Physics and Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China

    Shouyu Wang, Hailong Li, Jiancheng Lai, Yang Song & Zhenhua Li

  2. College of Electronics and Information Engineering, Shanghai University of Electric Power, Shanghai, 200090, China

    Liang Xue

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  1. Shouyu Wang
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  2. Liang Xue
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  3. Hailong Li
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  4. Jiancheng Lai
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  5. Yang Song
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  6. Zhenhua Li
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Corresponding author

Correspondence to Jiancheng Lai.

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Shouyu Wang and Liang Xue have contributed equally to this work.

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Wang, S., Xue, L., Li, H. et al. Quantitative phase detection with expanded principal component analysis method on interferometric microscopic cytometer. Appl. Phys. B 116, 235–239 (2014). https://doi.org/10.1007/s00340-013-5680-2

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  • DOI: https://doi.org/10.1007/s00340-013-5680-2

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