Skip to main content
SpringerLink
Log in

Antimicrobial, antioxidant, and anticancer potentials of AgCl nanoparticles biosynthesized by Flavobacterium panacis

  • Published:
Applied Physics A Aims and scope Submit manuscript

Abstract

Silver chloride nanoparticles (AgClNps) were biosynthesized extracellularly by Flavobacterium panacis (DCY106T), which was isolated from the rhizosphere of Panax ginseng in Gochang, Republic of Korea. The reduction of silver nitrate by DCY106T was initiated within 24 h at 37 °C. The complete reaction did not require supplemental reducing or stabilizing agents, further demonstrating the facile and simple synthesis of this methodology. The formation of nanoparticles was confirmed by UV–Vis spectroscopy, showing the maximum absorbance at ~ 402 nm. Field emission transmission electron microscopy revealed a spherical structure of the nanoparticles. Furthermore, the average size of AgClNps was around 20.66 nm as determined by X-ray diffraction. Fourier transform infrared spectroscopy analysis showed that functional biomolecules, such as proteins or enzymes from the cell-free supernatant of DCY106T, may provide nanoparticle stabilization against aggregations. In addition, the biosynthesized AgClNps served as competent antimicrobial agents against several pathogenic bacteria and fungi. Antioxidant results determined by 2, 2-diphenyl-1-picrylhydrzyl (DPPH) scavenging assay exhibited an efficient antioxidant activity of AgClNps. Moreover, AgClNps also revealed the anticancer potential against human lung cancer cells. Thus, this study proposes a facile extracellular synthesis of AgClNps by F. panacis (DCY106T) are applicable as the potential antimicrobial, antioxidant, and anticancer agents.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  1. N.D. Trinh, T.T.B. Nguyen, T.H. Nguyen, Adv. Nat. Sci. Nanosci. Nanotechnol. 6, 045011 (2015)

    Article  ADS  Google Scholar 

  2. R. Abbai, R. Mathiyalagan, J. Markus, Y.J. Kim, C. Wang, P. Singh, S. Ahn, M.E.A. Farh, D.C. Yang, Int. J. Nanomedicine. 11, 3131 (2016)

    Article  Google Scholar 

  3. Y. Huo, P. Singh, Y.J. Kim, V. Soshnikova, J.P. Kang, J. Markus, S. Ahn, V. Castro-Aceituno, R. Mathiyalagan, M. Chokkalingam, K.S. Bae, D.C. Yang, Artif. Cells. Nanomed. Biotechnol. 46, 303 (2018)

    Article  Google Scholar 

  4. R. Gengan, K. Anand, A. Phulukdaree, A. Chuturgoon, Colloids. Surf. B. Biointerfaces. 105, 87 (2013)

    Article  Google Scholar 

  5. A. Müller, D. Behsnilian, E. Walz, V. Gräf, L. Hogekamp, R. Greiner, Biocatal. Agric. Biotechnol. 6, 107 (2016)

    Article  Google Scholar 

  6. M.P. Patil, R.D. Singh, P.B. Koli, K.T. Patil, B.S. Jagdale, A.R. Tipare, G.D. Kim, Microb. Pathog. 121, 184 (2018)

    Article  Google Scholar 

  7. M.F. Lengke, M.E. Fleet, Southam. G Langmuir 23, 2694 (2007)

    Article  Google Scholar 

  8. A.R. Shahverdi, S. Minaeian, H.R. Shahverdi, H. Jamalifar, A.A. Nohi, Process. Biochem. 42, 919 (2007)

    Article  Google Scholar 

  9. M. Saravanan, S. Arokiyaraj, T. Lakshmi, A. Pugazhendhi, Microb. Pathog. 117, 68 (2018)

    Article  Google Scholar 

  10. M. Rai, S. Deshmukh, A. Ingle, A. Gade, J. Appl. Microbiol. 112, 841 (2012)

    Article  Google Scholar 

  11. Q.H. Tran, A.T. Le, Adv. Nat. Sci. Nanosci. Nanotechnol. 4, 033001 (2013)

    Article  ADS  Google Scholar 

  12. J. Venkatesan, S.K. Kim, M.S. Shim, Nanomaterials 6, 235 (2016)

    Article  Google Scholar 

  13. J.F. Bernardet, P. Segers, M. Vancanneyt, F. Berthe, K. Kersters, P. Vandamme, Int. J. Syst. Evol. Microbiol. 46, 128 (1996)

    Google Scholar 

  14. J.F. Bernardet, J.P. Bowman, in The prokaryotes. ed. by M. Dworkin, S. Falkow, E. Rosenberg, K.H. Schleifer, E. Stackebrandt (Springer, New York, 2006), p. 481

    Chapter  Google Scholar 

  15. D.H. Kim, P. Singh, M.E.A. Farh, Y.J. Kim, N.L. Nguyen, H.A. Lee, D.C. Yang, Antonie Van Leeuwenhoek 109, 1199 (2016)

    Article  Google Scholar 

  16. O.S. Kim, Y.J. Cho, K. Lee, S.H. Yoon, M. Kim, H. Na, S.C. Park, Y.S. Jeon, J.H. Lee, H. Yi, S.H. Won, J.S. Chun, J. Syst. Evol. Microbiol. 62, 716 (2012)

    Article  Google Scholar 

  17. J.P. Kang, Y.J. Kim, P. Singh, Y. Huo, V. Soshnikova, J. Markus, S. Ahn, M. Chokkalingam, H.A. Lee, D.C. Yang, Artif. Cells. Nanomed. Biotechnol. 46, 1530 (2018)

    Article  Google Scholar 

  18. L. Qi, Z. Xu, X. Jiang, C. Hu, X. Zou, Carbohydr. Res. 339, 2693 (2004)

    Article  Google Scholar 

  19. K. Paulkumar, S. Rajeshkumar, G. Gnanajobitha, M. Vanaja, C. Malarkodi, G. Annadurai, Int. J. Green. Chem. Bioprocess. 3, 12 (2013)

    Google Scholar 

  20. S. Desvani, I. Lestari, H. Wibowo, Supyani, S. H. Poromarto, Hadiwiyono. AIP Conference Proceedings. 2014, 020068 (2018)

  21. S. Guleria, R. Aggarwal, T. Thind, T. Sharma, J. Phytopathol. 155, 654 (2007)

    Article  Google Scholar 

  22. N. Khan, N. Jameel, J. Microb. Biochem. Technol. 8, 440 (2016)

    Article  Google Scholar 

  23. D.D. Wang, J. Markus, C. Wang, Y.J. Kim, R. Mathiyalagan, V.C. Aceituno, S. Ahn, D.C. Yang, Artif. Cells. Nanomed. Biotechnol. 45, 1548 (2017)

    Article  Google Scholar 

  24. V. Castro-Aceituno, R. Abbai, S.S. Moon, S. Ahn, R. Mathiyalagan, Y.J. Kim, Y.J. Kim, D.C. Yang, Biomed. Pharmacother. 93, 995 (2017)

    Article  Google Scholar 

  25. T. Ahmad, I.A. Wani, N. Manzoor, J. Ahmed, A.M. Asiri, Colloids. Surf. B. Biointerf. 107, 227 (2013)

    Article  Google Scholar 

  26. R. Bigdeli, M. Shahnazari, E. Panahnejad, R.A. Cohan, A. Dashbolaghi, V. Asgary, Artif. Cells. Nanomed. Biotechnol. 47, 1603 (2019)

    Article  Google Scholar 

  27. P. Singh, H. Singh, Y.J. Kim, R. Mathiyalagan, C. Wang, D.C. Yang, Enzyme. Microb. Technol. 86, 75 (2016)

    Article  Google Scholar 

  28. W. Wrótniak-Drzewiecka, S. Gaikwad, D. Laskowski, H. Dahm, J. Niedojadło, A. Gade, M. Rai, Austin. J. Biotechnol. Bioeng. 1, 1 (2014)

    Google Scholar 

  29. S. Rajeshkumar, C. Malarkodi. Bioinorg. Chem. Appl. 2014 (2014)

  30. G. Gnanajobitha, M. Vanaja, K. Paulkumar, S. Rajeshkumar, C. Malarkodi, G. Annadurai, C. Kannan, Int. J. Nanomater. Biostruct. 3, 21 (2013)

    Google Scholar 

  31. H. Bar, D.K. Bhui, G.P. Sahoo, P. Sarkar, S. Pyne, A. Misra, Surf. A. Physicochem. Eng. Asp. 348, 212 (2009)

    Article  Google Scholar 

  32. M. Sastry, A. Ahmad, M.I. Khan, R. Kumar, Curr. Sci. 85, 162 (2003)

    Google Scholar 

  33. A. Ahmad, Y. Wei, F. Syed, K. Tahir, A.U. Rehman, A. Khan, S. Ullah, Q. Yuan, Microb. Pathog. 102, 133 (2017)

    Article  Google Scholar 

  34. V. da Silva Ferreira, M.E. ConzFerreira, L.M.T. Lima, S. Frasés, W. de Souza, C. Sant’Anna, Enzyme Micro. Technol. 97, 114 (2017)

    Article  Google Scholar 

  35. D. Paredes, C. Ortiz, R. Torres, Int. J. nanomed. 9, 1717 (2014)

    Google Scholar 

  36. E.M. Halawani, J. Biomater. Nanobiotechnol. 8, 22 (2016)

    Article  Google Scholar 

  37. Q. Feng, J. Wu, G. Chen, F. Cui, T. Kim, J. Kim, J. Biomed. Mater. Res. 52, 662 (2000)

    Article  Google Scholar 

  38. J.R. Morones, J.L. Elechiguerra, A. Camacho, K. Holt, J.B. Kouri, J.T. Ramírez, M.J. Yacaman, Nanotechnology 16, 2346 (2005)

    Article  ADS  Google Scholar 

  39. I. Sondi, B. Salopek-Sondi, J. Colloid. Interface Sci. 275, 177 (2004)

    Article  ADS  Google Scholar 

  40. W. Hu, S. Chen, X. Li, S. Shi, W. Shen, X. Zhang, H. Wang, Mater. Sci. Eng. C. 29, 1216 (2009)

    Article  Google Scholar 

  41. J. Markus, R. Mathiyalagan, Y.J. Kim, R. Abbai, P. Singh, S. Ahn, Z.E.J. Perez, J. Hurh, D.C. Yang, Enzyme. Microb. Technol. 95, 85 (2016)

    Article  Google Scholar 

  42. R. Foldbjerg, D.A. Dang, H. Autrup, Arch. Toxicol. 85, 743 (2011)

    Article  Google Scholar 

  43. V. Kathiravan, S. Ravi, S. Ashokkumar, Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 130, 116 (2014)

    Article  ADS  Google Scholar 

Download references

Acknowledgement

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2019R1A6A3A01094826).

Author information

Authors and Affiliations

  1. Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Yongin-Si, Gyeonggi-do, 17104, Republic of Korea

    Yue Huo, Jong Pyo Kang, Jian Yu Pu & Deok Chun Yang

  2. College of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, People’s Republic of China

    Yue Huo & Chun Hong Piao

  3. Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do, 17104, Republic of Korea

    Ya Xi Han, Priyanka Singh & Deok Chun Yang

Authors
  1. Yue Huo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ya Xi Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Priyanka Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jong Pyo Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jian Yu Pu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chun Hong Piao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Deok Chun Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Chun Hong Piao or Deok Chun Yang.

Ethics declarations

Conflict of interest

The authors report no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Huo, Y., Han, Y.X., Singh, P. et al. Antimicrobial, antioxidant, and anticancer potentials of AgCl nanoparticles biosynthesized by Flavobacterium panacis. Appl. Phys. A 127, 227 (2021). https://doi.org/10.1007/s00339-021-04386-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00339-021-04386-z

Keywords

Search

Navigation