Skip to main content
SpringerLink
Log in

Linkage assignment of eleven genes to the porcine genome

  • Original Contribution
  • Published:
Mammalian Genome Aims and scope Submit manuscript

Abstract

We report comparative linkage mapping of eleven genes in the swine genome by RFLP analysis. These genes include: Acid phosphatase type 5 (ACP5), Cholecystokinin Type B Receptor (CCKBR), Antibiotic Peptide (FALL39), Insulin-like Growth Factor 1 Receptor (IGF1R), Integrin Alpha M (ITGAM), Integrin Beta 2 (ITGβ2), Opioid Receptor Mu-1 (OPRM1), Prohormone Converter (PCI/3), Retinol Binding Protein 3 (RBP3), Ribosomal DNA (RNR1), and Zona Pellucida Glycoprotein 1 (ZP1). The CCKBR and ITGβ2 loci define the ends of the linkage groups on Chromosomes (Chro) (SSC) 9p and 13qter, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Archibald AL, Haley CS, Brown JF, Couperwhite S, McQueen HA, Nicholson D, Coppieters W, Van de Weghe A, Stratil A, Wintero AK, Fredholm M, Larsen NJ, Nielson NH, Milan D, Woloszyn N, Robic A, Dalens M, Riquet J, Gellin J, Caritez J-C, Burgaud G, Ollivier L, Bidanel J-P, Vaiman M, Renard C, Geldermann H, Davoli R, Ruyter D, Verstege EJM, Groenen MAM, Davies W, Hoyheim B, Keiserud A, Andersson L, Ellegren H, Johansson M, Marklund L, Miller JR, Anderson Dear DV, Signer E, Jeffreys AJ, Moran C, Le Tissier P, Muladno, Rothschild MF, Tuggle CK, Vaske D, Helm J, Liu H-C, Rahman A, Yu P-T, Larson RG, Schmitz CB (1995) The PigMap consortium linkage map of the pig (Sus scrofa). Mamm Genome 6, 157–175

    Article  PubMed  CAS  Google Scholar 

  • Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32, 314–331

    PubMed  CAS  Google Scholar 

  • Dai G, Smeekens SP, Steiner DF, McMurtry JP, Kwok SCM (1995) Characterization of multiple prohormone convertase PC 1/3 transcripts in porcine ovary. Biochim Biophys Acta 1264, 1–6

    PubMed  Google Scholar 

  • Eggen A, Fries R (1995) An integrated cytogenetic and meiotic map of the bovine genome. Anim Genet 26, 215–236

    PubMed  CAS  Google Scholar 

  • Ellegren H, Chowdhary BP, Johansson M, Marklund L, Fredholm M, Gustavsson I, Andersson L (1994) A primary linkage map of the porcine genome reveals a low rate of genetic recombination. Genetics 137, 1089–1100

    PubMed  CAS  Google Scholar 

  • Feinberg AP and Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137, 226–267

    Google Scholar 

  • Fries R (1993) Mapping the bovine genome, methodological aspects and strategy. Anim Genet 24, 111–116

    PubMed  CAS  Google Scholar 

  • Fronicke L, Chowdhary BP, Scherthan H, Gustavsson I (1996) A comparative map of the porcine and human genomes demonstrates ZOO-FISH and gene mapping-based chromosomal homologies. Mamm Genome 7, 285–290

    Article  PubMed  CAS  Google Scholar 

  • Genome Database (1996) An international collaboration in support of the Human Genome Project [Online]. Available URL, http:// gdbwww.gdb.org Directory, gdb-bin/gdb5.6/lite/bin/genes. Hosted by Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Google Scholar 

  • Green P, Falls K, Crooks S (1989) Documentation for CRIMAP, version 2.4. (St. Louis: Washington University School of Medicine)

    Google Scholar 

  • Goureau A, Yerle M, Schmitz A, Riquet J, Milan D, Pinton P G. Frelat Gellin J (1996) Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36, 252–262

    Article  PubMed  CAS  Google Scholar 

  • Gudmundsson GH, Magnusson KP, Chowdhary BP, Johansson M, Andersson L, Boman HG (1995) Structure of the gene for porcine peptide antibiotic PR-39, a cathelin gene family member, comparative mapping of the locus for the human peptide antibiotic FALL-39. Proc Natl Acad Sci USA 92, 7085–7089

    Article  PubMed  CAS  Google Scholar 

  • Harney JP, Smith LC, Simmen RCM, Fliss AE, Bazer FW (1994) Retinolbinding protein, immunolocalization of protein and abundance of messenger ribonucleic acid in conceptus and maternal tissues during pregnancy in pigs. Biol Reprod 50, 1126–1135

    Article  PubMed  CAS  Google Scholar 

  • Johansson M, Ellegren H, Andersson L (1995) Comparative mapping reveals extensive linkage conservation—but with gene order rearrangements—between the pig and the human genomes. Genomics 25, 682–690

    Article  PubMed  CAS  Google Scholar 

  • Keele JW, Wray JE, Behrens DW, Rohrer GA, Sunden SLF, Kappes SM, Bishop MD, Stone RT, Alexander LJ, Beattie CW (1994) A conceptual database model for genomic research. J Comput Biol 1, 65–76

    PubMed  CAS  Google Scholar 

  • Lahbib-Mansais Y, Yerle M, Gellin J (1995) Localization of IGF1R and EDN genes to pig chromosomes 1 and 7 by in situ hybridization. Cytogenet Cell Genet 71, 225–227

    Article  PubMed  CAS  Google Scholar 

  • Ling X, Arnheim N (1994) Cloning and identification of the pig ribosomal gene promoter. Gene 150, 375–379

    Article  PubMed  CAS  Google Scholar 

  • Marklund L, Johansson Möller M, Høyheim B, Davies W, Fredholm M, Juneja RK, Mariani P, Coppieters W, Ellegren H, Andersson L (1996) A comprehensive linkage map of the pig based on a wild pig-Large White intercross. Anim Genet 27, 255–269

    Article  PubMed  CAS  Google Scholar 

  • Montgomery GW, Penty J, Hery HM, Sise J, Lord EA, Dodds K, Hill DF (1995) Sheep linkage mapping, RFLP markers for comparative mapping studies. Anim Genet 26, 249–259

    PubMed  CAS  Google Scholar 

  • Mouse Genome Informatics (1996) The mouse genome database [Online]. Available URL, http://www.informatics.jax.org. Directory, mgd.html. Hosted by Jackson Laboratory, Bar Harbor, ME, USA

    Google Scholar 

  • O’Brien SJ (1991) Mammalian genome mapping: lessons and prospects. Curr Opin Genet Dev 1, 105–111

    Article  PubMed  Google Scholar 

  • Rettenberger G, Klett C, Zechner U, Kunz J, Vogel W, Hameister H (1995) Visualization of the conservation of synteny between humans and pigs by heterologous chromosomal painting. Genomics 26, 372–378

    Article  PubMed  CAS  Google Scholar 

  • Rettenberger G, Bruch J, Fries R, Archibald AL, Hameister H (1996) Assignment of 19 porcine type I loci by somatic cell hybrid analysis detects new regions of conserved synteny between human and pig. Mamm Genome 7, 275–279

    Article  PubMed  CAS  Google Scholar 

  • Rohrer GA, Alexander LJ, Keele JW, Smith TP, Beattie CW (1994) A microsatellite linkage map of the porcine genome. Genetics 136, 231–245

    PubMed  CAS  Google Scholar 

  • Rohrer GA, Alexander LJ, Hu Z, Smith TPL, Keele JW, Beattie CW (1996) A comprehensive map of the porcine genome. Genome Res 6, 371–391

    Article  PubMed  CAS  Google Scholar 

  • Rothschild M, Jacobson C, Vaske D, Tuggle C, Wang L, Short T, Eckardt G, Sasaki S, Vincent A, McLaren D, Southwood O, Van der Steen H, Mileham A, Plastow G (1996) The estrogen receptor locus is associated with a major gene influencing litter size in pigs. Proc Natl Acad Sci USA 93, 201–205

    Article  PubMed  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning, A Laboratory Manual, 2nd ed. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press)

    Google Scholar 

  • Simmen RCM, Baumbach GA, Roberts RM (1988) Molecular cloning and temporal expression during pregnancy of the messenger ribonucleic acid encoding uteroferrin, a progesterone-induced uterine secretory protein. Mol Endocrinol 2, 2253–2262

    Article  Google Scholar 

  • Song I, Brown DR, Wiltshire RN, Gantz I, Trent JM, Yamada T (1993) The human gastrin/cholecystokinin type B receptor gene: alternative splice donor site in exon 4 generates two variant mRNAs. Proc Natl Acad Sci USA 90, 9085–9089

    Article  PubMed  CAS  Google Scholar 

  • Storici P, Zanetti M, (1993) A novel cDNA sequence encoding a pig leukocyte antimicrobial peptide with a cathelin-like pro-sequence. Biochem Biophys Res Commun 196, 1363–1368

    Article  PubMed  CAS  Google Scholar 

  • Taya T, Yamasaki N, Tsubamoto H, Hasegawa A, Koyama K (1995) Cloning of a cDNA coding for porcine zona pellucida glycoprotein ZP1 and its genomic organization. Biochem Biophys Res Commun 207, 790–799

    Article  PubMed  CAS  Google Scholar 

  • Womack JE (1987) Comparative gene mapping, a valuable tool for mammalian development studies. Dev Genet 8, 281–293

    Article  PubMed  CAS  Google Scholar 

  • Yasue H, Kusumoto H, Mikami H (1995) Assignment of the uteroferrin gene (ACP5) to swine chromosome 2q12 → q21 by fluorescence in situ hybridization. Cytogenet Cell Genet 71, 249–252

    Article  PubMed  CAS  Google Scholar 

  • Yerle M, Lahbib-Mansais Y, Mellink C, Goureau A, Pinton P, Echard G, Gellin J, Zijstra C, Haan ND, Bosma AA, Chowdhary B, Gu F, Gustavsson I, Thomsen PD, Christensen K, Rettenberger G, Hameister H, Schmitz A, Chaput B, Frelat G (1995) The PiGMaP consortium cytogenetic map of the domestic pig (Sus scrofa domestica). Mamm Genome 6, 176–186

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. U.S. Department of Agriculture, ARS, U.S. Meat Animal Research Center (MARC), Spur 18 D, P.O. Box 166, 68933-0166, Clay Center, Nebraska, USA

    Zhiliang Hu, Gary A. Rohrer, Roger T. Stone & Craig W. Beattie

  2. Department of Veterinary and PathoBiology, University of Minnesota, 66108, St. Paul, Minnesota, USA

    Mark Rutherford, Mark A. Osinski, Mary S. Pampusch, Michael P. Murtaugh & David R. Brown

  3. Dept. Animal Science, Iowa State University, 50011-3150, Ames, IA, USA

    Zhiliang Hu

  4. Department of Veterinary and Pathobiology, University of Minnesota, 66108, St. Paul, MN, USA

    Craig W. Beattie

Authors
  1. Zhiliang Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gary A. Rohrer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Roger T. Stone
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mark Rutherford
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mark A. Osinski
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mary S. Pampusch
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Michael P. Murtaugh
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. David R. Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Craig W. Beattie
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hu, Z., Rohrer, G.A., Stone, R.T. et al. Linkage assignment of eleven genes to the porcine genome. Mammalian Genome 8, 559–563 (1997). https://doi.org/10.1007/s003359900503

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s003359900503

Keywords

Search

Navigation