Abstract
We have generated a high-resolution genetic map, 0.071 cM per backcross animal, of the 13 cM T-H2 region of the mouse Chromosome (Chr) 17. The map contains two phenotypic loci, T and Hst1, 12 RFLP markers, and 24 microsatellite loci. The Hst1 gene was mapped to a chromosomal interval contained within a single 580-kb YAC clone. The FFEH11 YAC is 0.44 cM long and carries, besides the Hst1 gene, five polymorphic DNA markers and recombination breakpoints of six backcross animals. Two candidate genes for Hst1 were identified based on their location and testicular expression. These are Tbp and D17Ph4e. The submilliMorgan map of the T-H2 region revealed significant clustering of (CA)n loci. The clustering, if shown to be a common feature in the mouse genome, may cause gaps in the physical map of the mouse genome.
Similar content being viewed by others
References
Bonhomme, F., Guénet, J.-L., Catalan, J. (1982). Présence d’un facteur de stérilité mâle, Hst-2, segrégant dans les croisements interspécifiques M. musculus L. × M. spretus Lastaste et lié à Mod-1 et Mpi-1 sur le Chromosome 9. C.R. Acad. Sci. III, 691–693.
Chubb, C., Nolan, C. (1987). Mouse hybrid sterility and testicular function. Biol. Reprod. 36, 1343–1348.
Copeland, N.G., Jenkins, N.A., Gilbert, DJ., Eppig, J.T., Maltais, L.J., Miller, J.C., Dietrich, W.F., Weaver, A., Lincoln, S.E., Steen, R.G., Stein, L.D., Nadeau, J.H., Lander, E.S. (1993). A genetic linkage map of the mouse: current applications and future prospects. Science 262, 57–66.
Coyne, J.A. (1989). Two rules of speciation. In Speciation and Its Consequences, D. Otte, J.A. Endler, eds. (Sunderland, Mass.: Sinauer Associates), pp. 180–207.
Dietrich, W., Katz, H., Lincoln, S., Shin, H.-S., Friedman, J., Dracopoli, N.C., Lander, E. (1992). A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131, 423–447.
Dietrich, W.F., Miller, J.C., Steen, R.G., Merchant, M., Damron, D., Nahf, R., Gross, A., Joyce, D.C., Wessel, M., Dredge, R.D., Marquis, A., Stein, L.D., Goodman, N., Page, D.C., Lander, E. (1994). A genetic map of the mouse with 4,006 simple sequence length polymorphisms. Nature Genet. 7, 220–245.
Dobzhansky, T. (1951). Genetics and the Origin of Species. (New York: Columbia University).
Ebersole, T., Lai, F., Artzt, K. (1992). New molecular markers for the distal end of the t-complex and their relationships to mutations affecting mouse development. Genetics 131, 175–182.
Forejt, J. (1981). Hybrid sterility gene located in the T/t-H2 supergene on chromosome 17. In Current Trends in Histocompatibility, R.S. Reisfeld, R.A. Ferone, eds. (New York: Plenum Press), pp. 101–131.
Forejt, J. (1985). Chromosomal and genic sterility of hybrid type in mice and men. Exp. Clin. Immunogenet. 2, 106–19.
Forejt, J., Iványi, P. (1975). Genetic studies on male sterility of hybrids between laboratory and wild mice (Mus musculus L.). Genet. Res. 24, 189–206.
Forejt, J., Gregorova, S., Jansa, P. (1988). Three new t-haplotypes of Mus musculus reveal structural similarities to t-haplotypes of Mus domesticus. Genet. Res. 51, 111–119.
Forejt, J., Vincek, V., Klein, J., Lehrach, H., Loudova-Mickova, M. (1991). Genetic mapping of the t-complex region on mouse Chromosome 17 including the hybrid sterility-1 gene. Mamm. Genome 1, 86–94.
Forejt, J., Artzt, K., Barlow, D.P., Hamvas, R.M.J., Lindahl, K.F., Lyon, M.F., Klein, J., Silver, L.M. (1994). Mouse Chromosome 17. Mamm. Genome 5(Suppl.), S238-S258.
Guénet, J.-L., Nagamine, C., Simon-Chazottes, D., Montagutelli, X., Bonhomme, F. (1990). Hst3: an X-linked hybrid sterility gene. Genet. Res. 56, 163–165.
Haidane, J.B.S. (1922). Sex ratio and unisexual sterility in hybrid animals. J. Genet. 12, 101–109.
Hamada, H., Petrino, M.G., Kakunaga, T. (1982). A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes. Proc. Natl. Acad. Sci. USA 79, 6465–6469.
Heine, D., Khambata, S., Wydner, K.S., Passmore, H.C. (1994). Analysis of recombinational hot spots associated with the p haplotype of the mouse MHC. Genomics 23, 168–177.
Herrmann, B., Búcan, M., Mains, P.E., Frischauf, A.-M., Silver, L.M., Lehrach, H. (1986). Genetic analysis of the proximal portion of the mouse t complex: evidence for a second inversion within t haplotypes. Cell 44, 469–476.
Herrmann, B.G., Labeit, S., Poustka, A., King, T., Lehrach, H. (1990). Cloning of the T gene required in mesoderm formation in the mouse. Nature 343, 617–622.
Himmelbauer, H., Silver, L.M. (1993). High resolution comparative mapping of mouse Chromosome 17. Genomics 17, 110–120.
Jakobovits, A., Moore, A.L., Green, L.L., Vergara, G.J., Maynard-Currie, C.E., Austin, H.A., Klapholz, S. (1993). Germ-line transmission and expression of a human-derived yeast artificial chromosome. Nature 362. 255–258.
King, T.R., Dove, W.F., Herrmann, B., Moser, A.R., Shedlovsky, A. (1989). Mapping to molecular resolution in the T to H2 region of the mouse genome with a nested set of meiotic recombinants. Proc. Natl. Acad. Sci. USA 86, 222–226.
Kvist, S., Roberts, L., Dobberstein, B. (1983). Mouse histocompatibility genes: structure and organisation of a Kd gene. EMBO J. 2, 245–254.
Leder, A., Swan, D., Ruddle, F., D’Eustachio, P., Leder, P. (1981). Dispersion of alpha-like globin genes of the mouse to three different chromosomes. Nature 293, 196–200.
Love, J.M., Knight, A.M., McAleer, M.A., Todd, J.A. (1990). Towards construction of a high resolution map of the mouse genome using PCR-analyzed microsatellites. Nucleic Acids Res. 18, 4123–1130.
Mann, E.A., Silver, L.M., Elliot, R.W. (1986). Genetic analysis of a mouse t complex locus that is homologous to a kidney cDNA clone. Genetics 114, 993–1006.
Mosinger, B.J., Kralova, J., Forejt, J. (1988). A cloned H2 class I gene from a tw32-derived recombinant t haplotype identified as functional H2Kq gene. Immunogenetics 28, 283–285.
Nadeau, J.H., Phillips, S.J. (1987). The putative oncogene Pim-1 in the mouse: its linkage and variation among t haplotypes. Genetics 117, 533–541.
Nadeau, J.H., Herrmann, B., Bucan, M., Burkart, D., Crosby, J.L., Erhart, M.A., Kosowsky, M., Kraus, J.P., Michiels, F., Schnattinger, A., Tchetgen, M.-B., Varnum, D., Willison, K., Lehrach, H., Barlow, D. (1991). Genetic maps of mouse Chromosome 17 including 12 new anonymous DNA loci and 25 anchor loci. Genomics 9, 78–89.
Pavljukova, H., Forejt, J. (1981). Private communication. Mouse News Lett. 65, 44.
Perez, D.E., Wu, C.-I. (1995). Further characterization of the Odysseus locus of hybrid sterility in Drosophila: one gene is not enough. Genetics 140, 201–206.
Perez, D.E., Wu, C.-I., Johnson, N.A., Wu, M.-L. (1993). Genetics of reproductive isolation in the Drosophila simulans clade: DNA markerassisted mapping and characterization of a hybrid-male sterility gene, Odysseus (Ods). Genetics 134, 261–275.
Pilder, S.H., Hammer, M.F., Silver, L.M. (1991). A novel mouse Chromosome 17 hybrid sterility locus: implications for the origin of t haplotypes. Genetics 129, 237–246.
Pilder, S.H., Olds-Clarke, P., Phillips, D.M., Silver, L.M. (1993). Hybrid sterility-6: a mouse t complex locus controlling sperm flagellar assembly and movement. Dev. Biol. 159, 631–642.
Rappold, G.A., Stubbs, L., Labeit, S., Crkvenjakov, R.B., Lehrach, H. (1987). Identification of a testis-specific gene from the mouse t-complex next to a CpG-rich island. EMBO J. 6, 1975–1980.
Reeves, R.H., Crowley, M.R., O’Hara, B.F., Gearhart, J.D. (1990). Sex, strain, and species differences affect recombination across an evolutionarily conserved segment of mouse Chromosome 16. Genomics 8, 141–148.
Reeves, R.H., Crowley, M.R., Moseley, W.S., Seidin, M.F. (1991). Comparison of interspecific to intersubspecific backcrosses demonstrates species and sex differences in recombination frequency on mouse Chromosome 16. Mamm. Genome 1, 158–164.
Schedl, A., Montoliu, L., Kelsey, G., Schütz, G. (1993). A yeast artificial chromosome covering the tyrosinase gene confers copy numberdependent expression in transgenic mice. Nature 362, 258–261.
Silver, L.M. (1992). Bouncing off microsatellites. Nature Genet. 2, 8–9.
Strauss, W.M., Dausman, J., Beard, C., Johnson, C., Lawrence, J.B., Jaenisch, R. (1993). Germ line transmission of a yeast artificial chromosome spanning the murine alpha 1 (I) collagen locus. Science 259, 1904–1907.
Sutherland, H.F., Pick, E., Francis, F., Lehrach, H., Frischauf, A.-M. (1995). Mapping around the Fused locus on mouse Chromosome 17. Mamm. Genome 6, 449–153.
Tagle, D.A., Swaroop, M., Lovett, M., Collins, F.S. (1993). Magnetic bead capture of expressed sequences encoded within large genomic segments. Nature 361, 751–753.
Trachtulec, Z., Vincek, V., Hamvas, R.M.J., Forejt, J., Lehrach, H., Klein, J. (1994). Physical map of mouse Chromosome 17 in the region relevant for positional cloning of the hybrid sterility 1 gene. Genomics 23, 132–137.
Willison, K.R., Dudley, K., Potter, J. (1986). Molecular cloning and sequence analysis of a haploid expressed gene encoding t complex polypeptide-1. Cell 44, 727–738.
Wu, C.-I., Palopoli, M.F. (1994). Genetics of postmating reproductive isolation in animals. Annu. Rev. Genet. 27, 283–308.
Xiang, K., Cox, N.J., Hallewell, R.A., Bell, G.I. (1987). Multiple Taq I RFLPs at the human manganese Superoxide dismutase (Sod-2) locus on Chromosome 6. Nucleic Acids Res. 15, 7654.
Yoshiki, A., Moriwaki, K., Sakakura, T., Kusakabe, M. (1993). Histological studies on male sterility of hybrids between laboratory and wild mouse strains. Dev. Growth Differ. 35, 271–281.
Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., Friedman, J.M. (1994). Positional cloning of the mouse obese gene and its human homologue. Nature 372, 425–432.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gregorová, S., Mňuková-Fajdelová, M., Trachtulec, Z. et al. Sub-milliMorgan map of the proximal part of mouse chromosome 17 including the hybrid sterility 1 gene. Mammalian Genome 7, 107–113 (1996). https://doi.org/10.1007/s003359900029
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s003359900029