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Mapping of quantitative trait loci for mycoplasma and tetanus antibodies and interferon-gamma in a porcine F2 Duroc × Pietrain resource population

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Abstract

The aim of the present study was to detect quantitative trait loci (QTL) for innate and adaptive immunity in pigs. For this purpose, a Duroc × Pietrain F2 resource population (DUPI) with 319 offspring was used to map QTL for the immune traits blood antibodies and interferon-gamma using 122 microsatellites covering all autosomes. Antibodies response to Mycoplasma hyopneumoniae and tetanus toxoid vaccine and the interferon-gamma (IFNG) serum concentration were measured at three different time points and were used as phenotypes. The differences of antibodies and interferon concentration between different time points were also used for the linkage mapping. Line-cross and imprinting QTL analysis, including two-QTL, were performed using QTL Express. A total of 30 QTL (12, 6, and 12 for mycoplasma, tetanus antibody, and IFNG, respectively) were identified at the 5% chromosome-wide-level significant, of which 28 were detected by line-cross and 2 by imprinting model. In addition, two QTL were identified on chromosome 5 using the two-QTL approach where both loci were in repulsion phase. Most QTL were detected on pig chromosomes 2, 5, 11, and 18. Antibodies were increased over time and immune traits were found to be affected by sex, litter size, parity, and month of birth. The results demonstrated that antibody and IFNG concentration are influenced by multiple chromosomal areas. The flanking markers of the QTL identified for IFNG on SSC5 did incorporate the position of the porcine IFNG gene. The detected QTL will allow further research in these QTL regions for candidate genes and their utilization in selection to improve the immune response and disease resistance in pig.

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Acknowledgments

This project was supported by the Gene Dialog project, FUGATO Plus, BMBF, grant No. 0315130C, Germany. The authors are indebted to Miss Nadine Leyer, Institute of Animal Science, University of Bonn, Germany, for her help during the experiment.

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Authors and Affiliations

  1. Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany

    Muhammad Jasim Uddin, Christine Grosse-Brinkhaus, Mehmet Ulas Cinar, Elisabeth Jonas, Dawit Tesfaye, Ernst Tholen, Heinz Juengst, Christian Looft, Chirawath Phatsara & Karl Schellander

  2. Reprogen, University of Sydney, 425 Werombi Road, Camden, NSW, 2570, Australia

    Elisabeth Jonas

  3. Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany

    Siriluck Ponsuksili & Klaus Wimmers

  4. Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand

    Chirawath Phatsara

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  1. Muhammad Jasim Uddin
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  2. Christine Grosse-Brinkhaus
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  3. Mehmet Ulas Cinar
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Correspondence to Karl Schellander.

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335_2010_9269_MOESM1_ESM.tif

Supplementary file 1 Schematic display of vaccination program and time point of blood sampling from F2 DUPI population (TIFF 1049 kb)

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Uddin, M.J., Grosse-Brinkhaus, C., Cinar, M.U. et al. Mapping of quantitative trait loci for mycoplasma and tetanus antibodies and interferon-gamma in a porcine F2 Duroc × Pietrain resource population. Mamm Genome 21, 409–418 (2010). https://doi.org/10.1007/s00335-010-9269-3

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