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Identification of a 1.1-Mb region for a carcass weight QTL onbovine Chromosome 14

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Abstract

We previously mapped a quantitative trait locus for carcass weight, designated Carcass Weight-1 (CW-1), to bovine Chromosome 14 using a purebred Wagyu pedigree based on progeny design analysis. To refine the critical region within 8.1 cM flanked by microsatellites BMS1941 and INRA094, we constructed a bacterial artificial chromosome (BAC) contig composed of 60 tiled BAC clones and prepared a high-density physical map including 80 microsatellites, of which 55 were developed in this study. We conducted linkage disequilibrium (LD) mapping in the CW-1 region with 47 microsatellites using paternal half-sib pedigrees whose sires exhibited homozygous CW-1 Q alleles in the region. The LD mapping study significantly narrowed the CW-1 locus to the 1.1-Mb region between microsatellites DIK7012 and DIK7020. Finally, we surveyed the 1.1-Mb-region genotypes of 1700 steers from 11 bulls having −/−, Q/−, or Q/Q alleles in the region, and we examined the effect of the CW-1 Q allele on carcass weight. The presence of the first Q increased carcass weight by 23.6 kg (95% confidence interval [CI], 17.6–29.5 kg), and the second Q increased carcass weight an additional 15.2 kg (95% CI, 10.7–19.7 kg). These results indicate the presence of a gene responsible for carcass weight within the 1.1-Mb region.

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Acknowledgments

The authors thank H. Tsukazawa, T. Yamamoto, M. Nakatsugawa, Y. Arimura, and M. Hashimoto for technical assistance. The work was partly supported by the Ministry of Agriculture, Forestry, and Fishery, Japan, and by the Japan Racing and Livestock Promotion Foundation.

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Authors and Affiliations

  1. Cattle Breeding Development Institute of Kagoshima Prefecture, Tsukino, Osumi, So, Kagoshima, 899-8212, Japan

    Kazunori Mizoshita

  2. Shirakawa Institute of Animal Genetics, Japan Livestock Technology Association, Odakura, Nishigo, Fukushima, 961-8061, Japan

    Atsushi Takano, Toshio Watanabe, Akiko Takasuga & Yoshikazu Sugimoto

Authors
  1. Kazunori Mizoshita
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  2. Atsushi Takano
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  3. Toshio Watanabe
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  4. Akiko Takasuga
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  5. Yoshikazu Sugimoto
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Corresponding author

Correspondence to Yoshikazu Sugimoto.

Additional information

Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number AB204952-AB205006.

Kazunori Mizoshita, and Atsushi Takano contributed equally to this work.

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Mizoshita, K., Takano, A., Watanabe, T. et al. Identification of a 1.1-Mb region for a carcass weight QTL onbovine Chromosome 14. Mamm Genome 16, 532–537 (2005). https://doi.org/10.1007/s00335-005-0024-0

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  • DOI: https://doi.org/10.1007/s00335-005-0024-0

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