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Gene expression profiling of jejunal Peyer’s patches in juvenile and adult pigs

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Abstract

Peyer’s patches are organized lymphoid tissues of the small intestine that play a critical role in disease resistance and oral tolerance. Peyer’s patches in the jejunum contain lymphocytes, dendritic cells, macrophages, villous epithelium, and specialized follicle-associated epithelium. Little is known about the mechanisms and processes by which cells of the Peyer’s patches discriminate food nutrients and commensal microflora from pathogenic microbiota. We hypothesize that the jejunal Peyer’s patches express genes that mediate and regulate its essential functions. Expression patterns of approximately 2600 cDNAs from a porcine Peyer’s patch subtracted library were examined by microarray profiling. Individual mRNAs of interest were further examined by quantitative RT-PCR. Innate immunity-associated genes, including complement 3 and lysozyme, and the genes for epithelial chloride channel and trappin 1 were highly expressed by jejunal Peyer’s patch in both juvenile and adult pigs. The growth- and apoptosis-associated genes CIDE-B, GW112, and PSP/Reg I (pancreatic stone protein or regenerating gene) were differentially expressed in juvenile pig Peyer’s patches. Many sequences which were highly expressed in jejunal Peyer’s patches have previously been described with functions in epithelial cells. Animal-to-animal variation in basal jejunal Peyer’s patch gene expression was considerable and reflects the dynamic physiological environment of the gut in addition to genetic, epigenetic, and microbiological variation in the small intestine.

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Notes

  1. The TIGR Gene Index Databases, The Institute for Genomic Research, Rockville, MD 20850 (URL: website: http://www.tigr.org/tdb/tgi) (accessed August 2003).

Abbreviations

AE:

absorptive epithelium

EST:

expressed sequence tag

GALT:

gut-associated lymphoid tissue

JPP:

jejunal Peyer’s patches

IL:

interleukin

j-MLN:

jejunal mesenteric lymph node

LN:

lymph node

qRT-PCR:

quantitative reverse-transcriptase polymerase chain reaction

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Acknowledgments

The authors wish to thank Drs. Zheng Jin Tu and Wayne Xu at the University of Minnesota Supercomputing Institute for bioinformatics support and LaRae Peterson for skilled technical assistance and qRT-PCR expertise. This work was supported by the National Research Inititative of the USDA Cooperative State Research, Education and Extension Service, grant numbers 2002-35204-11705 and 2003-35205-2840 and the National Institutes of Health grant R01 DA-10200. KAH and CMTD were supported by the NIH/NIDA training grant T32-DA-07239.

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  1. Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, Minnesota, 55108, USA

    Juliana G. Machado, Kendra A. Hyland, Cheryl M.T. Dvorak & Michael P. Murtaugh

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  1. Juliana G. Machado
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  2. Kendra A. Hyland
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  3. Cheryl M.T. Dvorak
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  4. Michael P. Murtaugh
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Correspondence to Michael P. Murtaugh.

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Juliana G. Machado, Kendra A. Hyland and Michael P. Murtaugh contributed equally to this work.

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Machado, J.G., Hyland, K.A., Dvorak, C.M. et al. Gene expression profiling of jejunal Peyer’s patches in juvenile and adult pigs. Mamm Genome 16, 599–612 (2005). https://doi.org/10.1007/s00335-005-0008-0

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