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A novel mouse Chr5 locus Diht controls dopamine-induced hypothermia

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Abstract

Mice of the strain C3H.PRI-Flvr, carrying genetically determined resistance to flaviviruses, have been shown to be more sensitive to the hypothermic effect of dopamine than congenic flavivirus-susceptible C3H/HeJARC mice. In the current study, the greater sensitivity to dopamine-induced hypothermia observed in flavivirus-resistant mice was shown to be dose-dependent, with strain differences being the most prominent at a moderate dose of apomorphine (1 mg/kg). In addition, hypothermic responses to apomorphine were shown to be under developmental regulation; aging increased the potency of apomorphine-induced hypothermia and abrogated strain and sex differences observed in young mice. Linkage analysis of mouse strain-dependent co-inheritance between flavivirus resistance and greater sensitivity to the hypothermic effect of dopamine was performed using two genetically unrelated flavivirus-susceptible and two highly congenic flavivirus-resistant mouse strains in parallel with C3H.PRI-Flvr-and C3H/HeJARC reference strains. This study has revealed a clear segregation between flavivirus resistance conferred by the Flv locus and sensitivity to dopamine-controlled hypothermia conferred by a novel locus, Diht. Parallel studies in F1 and F2 heterozygote mice showed that the high sensitivity to hypothermic effect of dopamine (Dihthigh) is inherited as the Chr5-linked dominant trait. The novel locus, Diht, has been mapped proximal to the Flv locus on a distal part of mouse Chr5 between microsatellite markers D5Mit41 and D5Mit158.

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References

  • F Berrendero J Romero L Garcia–Gil I Suarez P La Cruz ParticleDe et al. (1998) ArticleTitleChanges in cannabinoid receptor binding and mRNA levels in several brain regions of aged rats Biochem Biophys Acta 1407 205–214 Occurrence Handle1:CAS:528:DyaK1cXls1Oltb0%3D Occurrence Handle9748581

    CAS  PubMed  Google Scholar 

  • AS Bloom KM Johnson WL Dewey (1978) ArticleTitleThe effects of cannabinoids on body temperature and brain catecholamine synthesis Res Commun Chem Pathol Pharmacol 20 51–57 Occurrence Handle1:CAS:528:DyaE1cXktlKktr0%3D Occurrence Handle663408

    CAS  PubMed  Google Scholar 

  • JS Brown SuffixJr (1994) ArticleTitleGeographic correlation of schizophrenia to ticks and tick-borne encephalitis Schizophr Bull 20 755–775 Occurrence Handle7701281

    PubMed  Google Scholar 

  • A Campbell RJ Baldessarini A Stoll MH Teicher P Maynard (1984) ArticleTitleEffect of age on behavioral responses and tissue levels of apomorphine in the rat Neuropharmacology 23 725–730 Occurrence Handle10.1016/0028-3908(84)90103-5 Occurrence Handle1:CAS:528:DyaL2cXltVCjs70%3D Occurrence Handle6540844

    Article  CAS  PubMed  Google Scholar 

  • E Carboni R Longoni S Deidda G Di Chiara (1986) ArticleTitleSCH23390 antagonises apomorphine- and ergot-induced hypothermia Eur J Pharmacol 125 17–22 Occurrence Handle10.1016/0014-2999(86)90078-6 Occurrence Handle1:CAS:528:DyaL28XksFCmsLY%3D Occurrence Handle3525197

    Article  CAS  PubMed  Google Scholar 

  • O Colboc J Costentin (1980) ArticleTitleEvidence for thermoregulatory dopaminergic receptors located in the preopticus medialis nucleus of the rat hypothalamus J Pharm Pharmacol 32 624–629 Occurrence Handle1:CAS:528:DyaL3MXivVWitQ%3D%3D Occurrence Handle6107363

    CAS  PubMed  Google Scholar 

  • J Costentin D Duterte–Boucher C Panissaud A Michael–Titus (1990) ArticleTitleDopamine D1 and D2 receptors mediate opposite effects of apomorphine on the body temperature of reserpinised mice Neuropharmacology 29 31–35 Occurrence Handle10.1016/0028-3908(90)90080-B Occurrence Handle1:CAS:528:DyaK3cXpt1ehtg%3D%3D Occurrence Handle1968237

    Article  CAS  PubMed  Google Scholar 

  • G Diaz–Veliz R Baeza F Benavente N Dussaubat S Mora (1994) ArticleTitleInfluence of the estrous cycle and estradiol on the behavioural effects of amphetamine and apomorphine in rats Pharmacol Biochem Behav 49 819–825 Occurrence Handle10.1016/0091-3057(94)90229-1 Occurrence Handle1:CAS:528:DyaK2MXitlCit7o%3D Occurrence Handle7886093

    Article  CAS  PubMed  Google Scholar 

  • WF Dietrich Miller JC RG Steen M Merchant D Damron et al. (1994) ArticleTitleA genetic map of the mouse with 4006 simple sequence length polymorphisms Nat Genet 7 220–245 Occurrence Handle1:CAS:528:DyaK2cXksFWhsL4%3D Occurrence Handle7920646

    CAS  PubMed  Google Scholar 

  • AM Ernst (1967) ArticleTitleMode of action of apomorphine and dexamphetamine on gnawing compulsion in rats Psychopharmacologia 10 316–323 Occurrence Handle10.1007/BF00403900 Occurrence Handle1:CAS:528:DyaF2sXnsVGmtg%3D%3D Occurrence Handle5627343

    Article  CAS  PubMed  Google Scholar 

  • SD Glick RG Marsanico (1974) ArticleTitleApomorphine-induced and pilocarpine-induced hypothermia in mice: Drug interactions and changes in drug sensitivity after caudate nucleus lesions Br J Pharmacol 51 353–357 Occurrence Handle1:CAS:528:DyaE2MXhtVKgsLY%3D Occurrence Handle4451749

    CAS  PubMed  Google Scholar 

  • GT Groschel H Koprowski (1965) ArticleTitleDevelopment of a virus-resistant inbred mouse strain for the study of innate resistance to arbo B viruses Arch Gesamte Virusforsch 17 379–391 Occurrence Handle1:STN:280:CCmC3szotFw%3D Occurrence Handle5882818

    CAS  PubMed  Google Scholar 

  • H Hermesh M Huberman H Radvan E Kott (1984) ArticleTitleRecurrent neuroleptic malignant syndrome due to tiapride and haloperidol: the possible role of D2 dopamine receptors J Nerv Ment Dis 172 692–695 Occurrence Handle1:STN:280:BiqD3s3msV0%3D Occurrence Handle6238132

    CAS  PubMed  Google Scholar 

  • H-K Jiang JF McGinty JS Hong (1990) ArticleTitleDifferential modulation of striatonigral dynorphin and enkephalin by dopamine receptor subtypes Brain Res 507 57 Occurrence Handle10.1016/0006-8993(90)90522-D Occurrence Handle1:CAS:528:DyaK3cXhtFantrw%3D Occurrence Handle1967976

    Article  CAS  PubMed  Google Scholar 

  • PE Keck SN Caroff SL McElroy (1995) ArticleTitleNeuroleptic malignant syndrome and malignant hyperthermia: end of a controversy? J Neuropsychiatry Clin Neurosci 7 135–144 Occurrence Handle7626956

    PubMed  Google Scholar 

  • HO Kiess (1989) Statistical Concepts for the Behavioral Sciences Allyn and Bacon Toronto

    Google Scholar 

  • KYH Lagerspetz H Koprowski M Darnell H Tarkkonen (1973) ArticleTitleThermoregulation in group B arbovirus-resistant and group B arbovirus-susceptible mice Am JPhysiol 225 532–537 Occurrence Handle1:STN:280:CSyB2c7ps1M%3D

    CAS  Google Scholar 

  • C-H Lammers U D’Souza Z-H Quin S-H Lee S Yajima et al. (1999) ArticleTitleRegulation of striatal dopamine receptors by estrogen Synapse 34 222–227 Occurrence Handle10.1002/(SICI)1098-2396(19991201)34:3<222::AID-SYN6>3.3.CO;2-A Occurrence Handle1:CAS:528:DyaK1MXntlGhur0%3D Occurrence Handle10523759

    Article  CAS  PubMed  Google Scholar 

  • MT Martin–Iverson (1999) ArticleTitleDoes sensitisation occur to prepulse inhibition of the startle reflex, effects of repeated apomorphine treatment in rats? J Psychopharmacol 13 261–273 Occurrence Handle1:CAS:528:DyaK1MXmslSrtLw%3D Occurrence Handle10512082

    CAS  PubMed  Google Scholar 

  • SA Miller DD Dykes HF Polesky (1988) ArticleTitleA simple salting out procedure for extracting DNA from human nucleated cells Nucleic Acids Res 16 1215 Occurrence Handle1:CAS:528:DyaL1cXhsVKlsrs%3D Occurrence Handle3344216

    CAS  PubMed  Google Scholar 

  • JL Nunes NA Sharif AD Michel RL Whiting (1991) ArticleTitleDopamine D2 receptors mediate hypothermia in mice: ICV and IP effects of agonists and antagonists Neurochem Res 16 1167–1174 Occurrence Handle1:CAS:528:DyaK3MXmslWht78%3D Occurrence Handle1686637

    CAS  PubMed  Google Scholar 

  • KJ Pittman HC Fibiger (1983) ArticleTitleThe effects of estrogen on apomorphine-induced hypothermia and stereotypy in the rat Neuropharmacology 22 587–592 Occurrence Handle10.1016/0028-3908(83)90149-1 Occurrence Handle1:CAS:528:DyaL3sXkvValurk%3D Occurrence Handle6683792

    Article  CAS  PubMed  Google Scholar 

  • R Rott S Herzog B Fleischer A Winokur J Amsterdam et al. (1985) ArticleTitleDetection of serum antibodies to Borna disease virus in patients with psychiatric disorders Science 228 755–756 Occurrence Handle1:STN:280:BiqC1M3ltlQ%3D Occurrence Handle3922055

    CAS  PubMed  Google Scholar 

  • P Salmi (1998) ArticleTitleIndependent roles of dopamine D1 and D2/3 s receptors in rat thermoregulation Brain Res 781 188–193 Occurrence Handle10.1016/S0006-8993(97)01229-8 Occurrence Handle1:CAS:528:DyaK1cXlsFSisA%3D%3D Occurrence Handle9507118

    Article  CAS  PubMed  Google Scholar 

  • C Sanchez (1989) ArticleTitleThe effects of dopamine D-1 and D-2 receptor agonists on body temperature in male mice Eur J Pharmacol 171 201–206 Occurrence Handle10.1016/0014-2999(89)90108-8 Occurrence Handle1:CAS:528:DyaK3cXkvFejtQ%3D%3D Occurrence Handle2575999

    Article  CAS  PubMed  Google Scholar 

  • OJ Silvia N Urosevic (1999) ArticleTitleVariations in LPS responsiveness among different mouse substrains of C3H lineage and their congenic derivative sublines Immunogenetics 50 354–357 Occurrence Handle10.1007/s002510050613 Occurrence Handle1:CAS:528:DyaK1MXnsFCrt7o%3D Occurrence Handle10630301

    Article  CAS  PubMed  Google Scholar 

  • MV Solbrig GF Koob JN Joyce W lan Lipkin (1996) ArticleTitleA neural substrate of hyperreactivity in Borna disease: Changes in brain dopamine receptors Virology 222 332–338 Occurrence Handle10.1006/viro.1996.0430 Occurrence Handle1:CAS:528:DyaK28XltVOgsL8%3D Occurrence Handle8806517

    Article  CAS  PubMed  Google Scholar 

  • AJ Stoessl MT Martin–Iverson TM Barth CT Dourish SD Iversen (1989) ArticleTitleEffects of ageing on the behavioural responses to dopamine agonists: decreased yawning and locomotion, but increased stereotypy Brain Res 495 20–30 Occurrence Handle10.1016/0006-8993(89)91214-6 Occurrence Handle1:CAS:528:DyaL1MXlsVKksbk%3D Occurrence Handle2570627

    Article  CAS  PubMed  Google Scholar 

  • R Strong T Samorajski Z Gottesfeld (1982) ArticleTitleRegional mapping of neostriatal neurotransmitter systems as a function of aging J Neurochem 39 831–836 Occurrence Handle1:CAS:528:DyaL38Xltl2hs7k%3D Occurrence Handle6124581

    CAS  PubMed  Google Scholar 

  • C Suaudeau R Bousselmame J Costentin (1992) ArticleTitleA different balance in the sensitivity of D1 and D2 dopamine receptors accounts for differences in apomorphine-induced hypothermic effects in Swiss and C3H mice Neuropharmacology 31 1115–1119 Occurrence Handle10.1016/0028-3908(92)90007-C Occurrence Handle1:CAS:528:DyaK38XmsVGmtr4%3D Occurrence Handle1475020

    Article  CAS  PubMed  Google Scholar 

  • NR Swerdlow DL Braff MA Geyer GF Koob (1986) ArticleTitleCentral dopamine hyperactivity in rats mimics abnormal acoustic startle response in schizophrenics Biol Psychiatry 21 23–33 Occurrence Handle10.1016/0006-3223(86)90005-3 Occurrence Handle1:CAS:528:DyaL28XlslSjs7g%3D Occurrence Handle3080033

    Article  CAS  PubMed  Google Scholar 

  • N Urosevic JP Mansfield JS Mackenzie GR Shellam (1995) ArticleTitleLow resolution mapping around the flavivirus resistance locus (Flv) on mouse Chromosome 5 Mamm Genome 6 454–458 Occurrence Handle1:CAS:528:DyaK2MXnvVakurk%3D Occurrence Handle7579886

    CAS  PubMed  Google Scholar 

  • N Urosevic OJ Silvia MY Sangster JP Mansfield SI Hodgetts et al. (1999) ArticleTitleDevelopment and characterization of new flavivirus-resistant mouse strains bearing Flv-like and Flvmr alleles from wild or wild-derived mice J Gen Virol 80 897–906 Occurrence Handle1:CAS:528:DyaK1MXitlGntrs%3D Occurrence Handle10211958

    CAS  PubMed  Google Scholar 

  • MW Vasey JF Thayer (1987) ArticleTitleThe continuing problem of false positives in repeated measures ANOVA in psychophysiology: A multivariate solution Psychophysiology 24 479–486 Occurrence Handle1:STN:280:BiiB1M%2FjslY%3D Occurrence Handle3615759

    CAS  PubMed  Google Scholar 

  • A Verma SK Kulkarni (1993) ArticleTitleDifferential role of dopamine receptor subtypes in thermoregulation and stereotypic behavior in naïve and reserpinised rats Arch Int Pharmacodyn 324 17–32 Occurrence Handle1:CAS:528:DyaK2cXht1en Occurrence Handle7905255

    CAS  PubMed  Google Scholar 

  • RW Waltrip SuffixII RW Buchanan A Summerfelt A Breier WT Carpenter SuffixJr et al. (1995) ArticleTitleBorna disease virus and schizophrenia Psychiatry Res 56 33–44 Occurrence Handle10.1016/0165-1781(94)02600-N Occurrence Handle7792340

    Article  PubMed  Google Scholar 

  • SW Young TI Bonner MR Brann (1986) ArticleTitleMesencephalic dopamine neurons regulate the expression of neuropeptide mRNAs in the rat forebrain Proc Natl Acad Sci USA 83 9827–9831 Occurrence Handle1:CAS:528:DyaL2sXmtFOitw%3D%3D Occurrence Handle2432603

    CAS  PubMed  Google Scholar 

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Acknowledgments

Funding for the project was gratefully received from the Western Australian Institute of Medical Research (WAIMR), Australia.

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Authors and Affiliations

  1. Western Australian Institute for Medical Research and UWA Centre for Medical Research, The University of Western Australia, B Block QEII Medical Centre, Monash Avenue, Nedlands, Western Australia, 6009, Australia

    Chris W. Stoddart, Mathew T. Martin–Iverson, Assen Jablensky & Nadezda Urosevic

  2. Pharmacology Unit, School of Medicine & Pharmacology, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia, 6009, Australia

    Chris W. Stoddart & Mathew T. Martin–Iverson

  3. Discipline of Microbiology, School of Biomedical and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia, 6009, Australia

    Nadezda Urosevic

  4. Centre for Clinical Research in Neuropsychiatry, Graylands-Hospital, Mt. Claremont, Western Australia, 6009, Australia

    Mathew T. Martin–Iverson & Assen Jablensky

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  1. Chris W. Stoddart
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  2. Mathew T. Martin–Iverson
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  3. Assen Jablensky
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  4. Nadezda Urosevic
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Correspondence to Nadezda Urosevic.

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Stoddart, C.W., Martin–Iverson, M.T., Jablensky, A. et al. A novel mouse Chr5 locus Diht controls dopamine-induced hypothermia. Mamm Genome 15, 901–913 (2004). https://doi.org/10.1007/s00335-004-2394-0

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  • DOI: https://doi.org/10.1007/s00335-004-2394-0

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