Abstract
Antarctica is a region with harsh conditions which have become a focus of research for identifying novel extreme microorganisms in recent years. Here, we identified and characterized the KGI_MA19 isolate obtained by enriched culture and freeze–thaw stress treatment from the sediment samples of King George Island in Antarctica. Whole-genome sequencing, MLSA, and phylogenetic analyses revealed that this isolate was a novel strain of Pseudomonas mandelii sharing less similarity to the first Pseudomonas mandelii isolate 6A1 from Antarctica. The phenotypic and biochemical characterizations indicate that this psychrotolerant and halotolerant strain can use disaccharides as a carbon source, has denitrification ability, and has multiple antibiotic resistance. Moreover, comparative proteomic analyses showed that P. mandelii KGI_MA19 adapts to the cold environment by changing its membrane composition, excreting EPS, and producing major cold shock proteins, in addition to turning off its other stress response mechanisms.
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Acknowledgements
This study was carried under the auspices of Presidency of The Republic of Turkey, supported by the Ministry of Industry and Technology, and coordinated by Istanbul Technical University (ITU) Polar Research Center (PolReC). This study was in the frame of bilateral agreement with Polish Institute of Biochemistry and Biophysics (IBB) and PolReC. We would like to thank to 42nd expedition crew for their hospitality and help in Henryk Arctowski Polish Antarctic Station.
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This project was supported by TUBITAK (the Scientific and Technological Research Council of Turkey) (Grant No. 119Y411) and ITU Scientific Research Projects Division (Grant Nos. 41729 and 42256).
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NGK and NB conceived the research. NB and YG collected the samples. HMT, ETSC, MMK, AC, and MOK conducted the laboratory work. HMT, IK, MOK, ETSC analyzed the data. HMT, ETSC, MMK, AC, YG, IK, and MOK wrote the original manuscript. All authors revised and approved the manuscript. NGK received funding from Istanbul Technical University, Scientific Research Projects Coordination Unit.
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Mumcu, H., Sarac Cebeci, E.T., Menekse Kılıc, M. et al. Identification of phenotypic and genotypic properties and cold adaptive mechanisms of novel freeze–thaw stress-resistant strain Pseudomonas mandelii from Antarctica. Polar Biol 46, 169–183 (2023). https://doi.org/10.1007/s00300-023-03114-y
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DOI: https://doi.org/10.1007/s00300-023-03114-y