Abstract
Logistics and scientific activities carried out in Antarctic stations entail the risk of contamination by fuels. Among remediation strategies, biostimulation significantly improves the efficiency of hydrocarbons (HCs) removal. A 1-year-long field trial was performed in mesocosms filled with soil chronically contaminated with HCs. Three nutrient sources were evaluated as biostimulation agents: inorganic salts (with and without aeration by mixing), a slow-release granular fertilizer (Nitrofoska®) and a commercial bioremediation product (OSEII®). Their performance was assessed considering the number of culturable bacteria, the changes induced in the structure of bacterial communities, the HCs removal efficiencies and the estimation of the abiotic and biodegradative losses of HCs. The soil indigenous microbiota reduced the concentration of hydrocarbons by up to 50% in 50 days and 87% in 365 days depending on the biostimulation agent used. OSEII® (a mixture of surfactants, nutrients, and enzymes) performed better in the medium term, promoting bacterial growth and rapidly inducing changes in the structure of bacterial community, and Nitrofoska® proved to be more efficient for long-term processes, less affecting the size and structure of the microbiota. A mixed strategy combining the fastest action of commercial products acting during summer with slow-release fertilizers acting throughout the year is proposed as a long-term bioremediation treatment for Antarctic areas where the temperature rises above the freezing point and the ground is free of snow shortly during summer. This study highlights the importance of conducting research to develop remediation processes compatible with the Antarctic Treaty, exploiting the metabolic potential of the indigenous microbiota.
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Acknowledgements
This work was supported by the Agencia Nacional de Promoción Científica y Tecnológica, ANPCyT (PICT 2016-7271) and the Universidad de Buenos Aires, UBA (UBACyT 20020130100569BA). We are grateful to the Instituto Antártico Argentino (IAA), Carlini Station, for their hospitality and logistic support and to Silvia H. Coria for her valuable support in the field and laboratory activities performed at Carlini Station. Dr. Daniel Delille and Dr. David Junk are thanked for critically reading the manuscript and suggesting substantial improvements.
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Villalba Primitz, J., Vázquez, S., Ruberto, L. et al. Bioremediation of hydrocarbon-contaminated soil from Carlini Station, Antarctica: effectiveness of different nutrient sources as biostimulation agents. Polar Biol 44, 289–303 (2021). https://doi.org/10.1007/s00300-020-02787-z
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DOI: https://doi.org/10.1007/s00300-020-02787-z