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Characterization of a novel antarctic plant growth-promoting bacterial strain and its interaction with antarctic hair grass (Deschampsia antarctica Desv)

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Abstract

Deschampsia antarctica is the only hair grass that has been able to successfully colonize the Antarctic continent. However, there is little research on the role of microorganisms associated with the rhizosphere that may participate in its growth and development. The objective of this research was to characterize a psychrotolerant bacterial strain isolated from the rhizosphere of D. antarctica. Biochemical and molecular studies were performed to characterize this bacterium. It was determined that this strain secretes a neutral polysaccharide that presents different compositions at different temperatures (4 and 20 °C). Based on biochemical and phylogenetic analyses, the Antarctic rhizobacterium could be a new species of Pseudomonas. To determine their ability to solubilize different sources of inorganic phosphate, qualitative and quantitative analyses were conducted to determine P released at 4 °C. The Antarctic strain of Pseudomonas sp. was able to solubilize all sources of phosphates, and 34.2 mg P/L was released from rock phosphate. Growth physiological parameters were evaluated for seedlings of D. antarctica inoculated with the rhizobacteria. It was found that the bacterial inoculation promoted plant root development. SEM analysis of the roots showed that the bacterium is mainly located in the root hairs of D. antarctica.

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Acknowledgments

The authors thank Ramon Rossello Mora for his assistance with phylogenetic analyses. The authors thank Charles Guy for his help in the translation and proofreading of the manuscript. Monica Ramirez and Yamile Bernardo are thanked for their assistance with HPLC Analyses. The authors thank INACH 0301 Grants; UXMAL S.A.: Doctorate Fellowship CONICYT Graciela Berríos.

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Authors and Affiliations

  1. Laboratorio de Fisiología y Biología Molecular Vegetal, Departamento de Producción Agropecuaria, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Casilla 54-D, Temuco, Chile

    Graciela Berríos & Ana Gutiérrez-Moraga

  2. Vicerrectoría de Investigación y Postgrado, Universidad de La Frontera, Casilla 54-D, Temuco, Chile

    Manuel Gidekel

  3. Insumos Orgánicos y Naturales para Agricultura y Jardinería (IONA), Carretera General San Martin km 24, Colina, Santiago, Chile

    Gustavo Cabrera

  4. Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Casilla 54-D, Temuco, Chile

    Graciela Berríos & Ana Gutiérrez-Moraga

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  1. Graciela Berríos
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  2. Gustavo Cabrera
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  3. Manuel Gidekel
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  4. Ana Gutiérrez-Moraga
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Correspondence to Ana Gutiérrez-Moraga.

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Berríos, G., Cabrera, G., Gidekel, M. et al. Characterization of a novel antarctic plant growth-promoting bacterial strain and its interaction with antarctic hair grass (Deschampsia antarctica Desv). Polar Biol 36, 349–362 (2013). https://doi.org/10.1007/s00300-012-1264-6

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  • DOI: https://doi.org/10.1007/s00300-012-1264-6

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