Abstract
Transgenic tobacco plants producing chloroperoxidase (CPO-P), encoded by a novel gene from Pseudomonas pyrrocinia, were obtained by Agrobacterium-mediated transformation. Successful transformation was shown by PCR, Southern, northern and western blot analyses, and assays of CPO-P enzyme activity. Extracts from plants transformed with the CPO-P gene significantly reduced Aspergillus flavus colonies by up to 100% compared with extracts from control plants transformed with pBI121. Compared with controls, the transformed plants showed increased disease resistance in planta against a fungal pathogen, Colletotrichum destructivum, the causal agent of tobacco anthracnose.
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Received: 10 March 1999 / Revision received: 22 June 1999 · Accepted: 5 July 1999
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Rajasekaran, K., Cary, J., Jacks, T. et al. Inhibition of fungal growth in planta and in vitro by transgenic tobacco expressing a bacterial nonheme chloroperoxidase gene. Plant Cell Reports 19, 333–338 (2000). https://doi.org/10.1007/s002990050736
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DOI: https://doi.org/10.1007/s002990050736