Abstract
The expression of the Arabidopsis heat-shock protein (HSP) 18.2 promoter β-d-glucuronidase (GUS) chimera gene was studied in various organs of the transgenic Nicotiana plumbaginifolia during the recovery phase at normal temperatures (20–22 °C) following heat-shock (HS) treatment. The optimum HS temperature for GUS activity in the anthers, petals and capsules was 42 °C, but in immature seeds and the placentas of capsules it was 36 °C and 39 °C, respectively. No apparent GUS activity was observed in any organs except for dry seeds after HS at 45 °C, although the activity in dry seeds was apparent even after HS at 48 °C. After HS at 42 °C, GUS activity in the flower tissues was the highest before anthesis and declined thereafter.
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Received: 13 January 1998 / Revision received: 25 January 1999 / Accepted: 3 March 1999
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Moriwaki, M., Yamakawa, T., Washino, T. et al. Organ-specific expression of β-glucuronidase activity driven by the Arabidopsis heat-shock promoter in heat-stressed transgenic Nicotiana plumbaginifolia. Plant Cell Reports 19, 92–95 (1999). https://doi.org/10.1007/s002990050716
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DOI: https://doi.org/10.1007/s002990050716